INFLUENCE OF ACETONE EXTRACT FROM SOME LICHENIZED FUNGI ON THE GROWTH ACTIVITY OF POPULUS TREMULA L. IN VITRO

D. N. Zontikov, S. Zontikova, K. V. Malakhova, Al'bert Vladimirovich Kanarskiy, Aleksey Aleksandrovich Timakov, Roman Vladimirovich Sergeyev
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Abstract

The article presents the results of studying the effect of extracts of epigeic lichenized fungi in concentrations from 10 to 50 mg/l on the activity of growth processes, gemmogenesis and rhizogenesis of the economically valuable species Populus tremula L. when cultivated in vitro. Epigeic and epiphytic lichenized fungi served as raw materials for the isolation of the acetone extract: Cetraria islandica (L.) Ach. families Parmeliaceae of the order Lecanorales of the department Lecanoromycetes and Evernia mesomorpha Nyl. – families Parmeliaceae of the order Lecanorales of the class Lecanoromycetes. Acetone was used as a solvent in the extraction of lichen acids from crushed raw materials of thalli. Under the influence of the allelopathic effect of the acetone extract of C. islandica and E. mesomorpha on the activity of growth processes and rhizogenesis of P. tremula, we studied the extract concentrations of 10, 30, and 50 mg/l of the nutrient medium. As a result, it was found that the concentration of the extract of 10 mg/l leads to an increase in the length of the microshoot and activation of the processes of gemmogenesis and rhizogenesis compared to the control in the absence of explant death. Significant differences were obtained in the variant using the acetone extract of C. islandica.
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一些地衣化真菌的丙酮提取物对杨树体外生长活性的影响
文章介绍了研究附生地衣真菌萃取物(浓度为 10 至 50 毫克/升)对经济珍贵树种杨树(Populus tremula L.)体外培养的生长过程活性、宝石生成和根茎生成的影响的结果。作为丙酮提取物分离原料的附生地衣和附生地衣真菌有:Cetraria islandica (L.) Ach.和 Evernia mesomorpha Nyl.- 在研究过程中,我们使用了丙酮作为溶剂。丙酮被用作溶剂,从粉碎的苔藓原料中提取地衣酸。在研究 C. islandica 和 E. mesomorpha 的丙酮提取物对震颤果生长过程和根茎生成活动的等位影响时,我们研究了营养培养基中 10、30 和 50 毫克/升的提取物浓度。结果发现,在没有外植体死亡的情况下,与对照组相比,浓度为 10 毫克/升的提取物会导致微芽长度增加,并激活宝石生成和根茎生成过程。在使用 C. islandica 的丙酮提取物的变体中也发现了显著差异。
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