A Study on Yield and Gut Flora Diversity on Vermicomposting of Bio-Waste Using Eisenia fetida

Rahtinabala Kanagaraj, Muthupandi Guru, Dorai Pandian Kannan
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Abstract

Background and Objective: Management of accumulated refuse materials can be made effective through vermicomposting. The study aims to find the yield of compost from different bio-wastes and biochemical characterization of microbes present in the gut region. Materials and Methods: Collected and dried biowastes were spread over concrete tanks for composting. Addition of cow dung slurry for pre-treating the biowastes. Eisenia fetida was inoculated into the tanks in a ratio of 10:1 and left for vermicomposting. About one gram of earthworm tissue was dissected and taken for analysis. Microbiological methods were employed to isolate, identify and characterize the vermin bacteria. Results: Degradation of hard fibrous sugarcane bagasse resulted relatively in a higher vermicompost production (55.79 and 70.34%) and an increased percentage (29%) of earthworm when compared to other wastes used in the study. As 70.34% recovery during the second study period was obtained because of the longer pre-composting period. Eight different bacterial isolates identified from the gut region were found to be present in the soil, which has plant growth-regulating functions. Conclusion: The results of the work can be applicable in multi-faceted fields, including natural resources conservation, organic farming, recycling and reuse of solid and agro-wastes.
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利用蚯蚓堆肥生物垃圾的产量和肠道菌群多样性研究
背景和目的:通过蚯蚓堆肥可以有效管理堆积的垃圾。本研究旨在了解不同生物垃圾的堆肥产量以及肠道微生物的生化特征。材料和方法:将收集和干燥的生物废料铺在混凝土槽中进行堆肥。加入牛粪泥浆对生物废料进行预处理。将蚯蚓按 10:1 的比例接种到池中,然后进行蚯蚓堆肥。大约一克的蚯蚓组织被剖开并进行分析。采用微生物学方法分离、鉴定和描述蚯蚓细菌。结果与研究中使用的其他废物相比,降解硬纤维甘蔗渣产生的蚯蚓堆肥产量相对较高(55.79% 和 70.34%),蚯蚓的比例也有所增加(29%)。在第二个研究阶段,由于堆肥前的时间较长,回收率达到了 70.34%。研究发现,土壤中存在 8 种从肠道中分离出来的细菌,它们具有调节植物生长的功能。结论这项工作的成果可应用于多个领域,包括自然资源保护、有机耕作、固体废物和农业废物的回收和再利用。
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