Determination of Antioxidant Activity of The Leaves of Hawthorn (Crataegus Monogyna)

Abstract

This study was carried out to make decision the antioxidant activity of the leaves of hawthorn (Crataegus monogyna), which is a natural source of antioxidants. For this purpose, the leaves of hawthorn were collected and dried, and an ethanol extract was prepared. Total antioxidant activity (with respect to the ferric thiocyanate method), ion reduction capacity of Cu2+ (copper method), and Fe3+ (potassium ferricyanide reduction method) were determined on the prepared ethanol extracts. Additionally, scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazyl) and determination of the total phenolic-flavonoid compound content were carry out. The extracts obtained from Hawthorn (Crataegus monogyna) leaves were found to inhibit lipid peroxidation (of linoleic acid emulsion) by 40.9%. This inhibition occurred at a concentration of 10 µg/mL of the extracts. At the similar concentration, BHT exhibited 68.3% inhibition, while trolox exhibited 29.9% inhibition. Hawthorn (Crataegus monogyna), which was found to have a higher total phenolic content than trolox, exhibited significant DPPH scavenging and metal reduction activities. During the investigation of the antioxidant activity of the ethanol extract obtained from Crataegus monogyna, α-tocopherol, its water-soluble analogue trolox and BHT (butylated hydroxytoluene) were utilized as reference antioxidant compounds.