EXPERIMENTAL STUDY OF ERYTHROCYTE ENERGY METABOLISM UNDER INHALATIONS OF NITRIC OXIDE

Abstract

Abstract Biological effects of nitric oxide are multiply, including vasoactive activity, participation in neurotransmission and intercellular communication etc. These effects are associated with endogenous releasing of nitric oxide, but influence of exogenous administration of this substance does not study in details. In particular, systemic action of nitric oxide inhalations is not so clear. The aim of this work was the investigation of nitric oxide inhalations action on some parameters of energy and oxidative metabolism of healthy rat blood. Wistar rats were randomly divided into two groups: control group (without any manipulations; n=10) and main group (n=10) with inhalations by nitric oxide-containing gas flow (20 ppm). Lactate dehydrogenase and lactate level were estimated in rat blood samples. In addition, we calculated a number of integral coefficients of energy metabolism, such as substrate provision coefficient and coefficient of energy reactions balance. Our experiments demonstrate that 10-days course of inhalations of low nitric oxide doses (20 ppm) increases the adaptive potential of healthy rats’ organism. One of these positive effects is associated with activation of some components of energy metabolism. First of all, it realized through stimulation of catalytic activity of lactate dehydrogenase, including its erythrocyte pool. Observed metabolic effect provides the basis for pathogenic correction of diseases, associated with hypoxia, oxidative stress and energy deficiency.