{"title":"Chitosan-capped CuO nanoparticles: a comprehensive, comparative study of their broad-spectrum antibacterial efficacy","authors":"Kanchan Parate, Damayanti Meher, Pallavi Gupta, Pratibha Pandey","doi":"10.1007/s13204-023-02987-z","DOIUrl":null,"url":null,"abstract":"<div><p>Antibacterial efficacy of chitosan-capped copper oxide nanoparticles (CuO–CS NPs) was evaluated against the Gram-negative bacteria (<i>E. coli</i>) and Gram-positive bacteria (<i>B. subtilis).</i> Comparative antibacterial activity evaluation was performed for CuO–CS NPs, nano Cu(OH)<sub>2,</sub> nano silver, CuO, and CuSO<sub>4</sub>.5H<sub>2</sub>O salt. Miles and Misra method and the spread plate CFU count method were used to enumerate cell counts for antibacterial activity evaluation. The spread plate colony forming unit (CFU) count method is preferable because of its reliability and accuracy. The minimum bactericidal concentration (MBC) of CuO–CS NPs were determined to be 2 µg/ml for <i>E. coli</i> and 12.5 µg/ml for<i> B. subtilis.</i> The values were comparable to those obtained for AgNPs. Moreover, in kinetic study these NPs showed the ability to decontaminate 2 × 10<sup>5</sup> CFU/ml of <i>E. coli</i> within 2 h at a concentration of 10 μg/ml and 3 × 10<sup>5</sup> CFU/ml of <i>B. subtilis</i> within 4 h at a concentration of 20 μg/ml. The potential application of synthesized CuO-CS NPS as an effective antibacterial nanocoating on fabrics was also demonstrated by using both qualitative and quantitative test methods.</p></div>","PeriodicalId":471,"journal":{"name":"Applied Nanoscience","volume":"14 2","pages":"441 - 455"},"PeriodicalIF":3.6740,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Nanoscience","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s13204-023-02987-z","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Engineering","Score":null,"Total":0}
引用次数: 0
Abstract
Antibacterial efficacy of chitosan-capped copper oxide nanoparticles (CuO–CS NPs) was evaluated against the Gram-negative bacteria (E. coli) and Gram-positive bacteria (B. subtilis). Comparative antibacterial activity evaluation was performed for CuO–CS NPs, nano Cu(OH)2, nano silver, CuO, and CuSO4.5H2O salt. Miles and Misra method and the spread plate CFU count method were used to enumerate cell counts for antibacterial activity evaluation. The spread plate colony forming unit (CFU) count method is preferable because of its reliability and accuracy. The minimum bactericidal concentration (MBC) of CuO–CS NPs were determined to be 2 µg/ml for E. coli and 12.5 µg/ml for B. subtilis. The values were comparable to those obtained for AgNPs. Moreover, in kinetic study these NPs showed the ability to decontaminate 2 × 105 CFU/ml of E. coli within 2 h at a concentration of 10 μg/ml and 3 × 105 CFU/ml of B. subtilis within 4 h at a concentration of 20 μg/ml. The potential application of synthesized CuO-CS NPS as an effective antibacterial nanocoating on fabrics was also demonstrated by using both qualitative and quantitative test methods.
期刊介绍:
Applied Nanoscience is a hybrid journal that publishes original articles about state of the art nanoscience and the application of emerging nanotechnologies to areas fundamental to building technologically advanced and sustainable civilization, including areas as diverse as water science, advanced materials, energy, electronics, environmental science and medicine. The journal accepts original and review articles as well as book reviews for publication. All the manuscripts are single-blind peer-reviewed for scientific quality and acceptance.