Chitosan-capped CuO nanoparticles: a comprehensive, comparative study of their broad-spectrum antibacterial efficacy

IF 3.674 4区 工程技术 Q1 Engineering Applied Nanoscience Pub Date : 2024-01-08 DOI:10.1007/s13204-023-02987-z
Kanchan Parate, Damayanti Meher, Pallavi Gupta, Pratibha Pandey
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Abstract

Antibacterial efficacy of chitosan-capped copper oxide nanoparticles (CuO–CS NPs) was evaluated against the Gram-negative bacteria (E. coli) and Gram-positive bacteria (B. subtilis). Comparative antibacterial activity evaluation was performed for CuO–CS NPs, nano Cu(OH)2, nano silver, CuO, and CuSO4.5H2O salt. Miles and Misra method and the spread plate CFU count method were used to enumerate cell counts for antibacterial activity evaluation. The spread plate colony forming unit (CFU) count method is preferable because of its reliability and accuracy. The minimum bactericidal concentration (MBC) of CuO–CS NPs were determined to be 2 µg/ml for E. coli and 12.5 µg/ml for B. subtilis. The values were comparable to those obtained for AgNPs. Moreover, in kinetic study these NPs showed the ability to decontaminate 2 × 105 CFU/ml of E. coli within 2 h at a concentration of 10 μg/ml and 3 × 105 CFU/ml of B. subtilis within 4 h at a concentration of 20 μg/ml. The potential application of synthesized CuO-CS NPS as an effective antibacterial nanocoating on fabrics was also demonstrated by using both qualitative and quantitative test methods.

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壳聚糖包裹的氧化铜纳米粒子:对其广谱抗菌功效的综合比较研究
评估了壳聚糖封端氧化铜纳米粒子(CuO-CS NPs)对革兰氏阴性菌(大肠杆菌)和革兰氏阳性菌(枯草杆菌)的抗菌效果。对 CuO-CS NPs、纳米 Cu(OH)2、纳米银、CuO 和 CuSO4.5H2O 盐进行了抗菌活性比较评估。评估抗菌活性时使用了 Miles 和 Misra 法以及展板菌落形成单位计数法来统计细胞数。涂布平板菌落形成单位(CFU)计数法因其可靠性和准确性而更受欢迎。经测定,CuO-CS NPs 对大肠杆菌的最小杀菌浓度(MBC)为 2 µg/ml,对枯草杆菌的最小杀菌浓度(MBC)为 12.5 µg/ml。这些数值与 AgNPs 的数值相当。此外,在动力学研究中,当浓度为 10 μg/ml 时,这些 NPs 能够在 2 小时内净化 2 × 105 CFU/ml 的大肠杆菌;当浓度为 20 μg/ml 时,这些 NPs 能够在 4 小时内净化 3 × 105 CFU/ml 的枯草杆菌。此外,还通过定性和定量测试方法证明了合成的 CuO-CS NPS 作为一种有效的织物抗菌纳米涂层的应用潜力。
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来源期刊
Applied Nanoscience
Applied Nanoscience Materials Science-Materials Science (miscellaneous)
CiteScore
7.10
自引率
0.00%
发文量
430
期刊介绍: Applied Nanoscience is a hybrid journal that publishes original articles about state of the art nanoscience and the application of emerging nanotechnologies to areas fundamental to building technologically advanced and sustainable civilization, including areas as diverse as water science, advanced materials, energy, electronics, environmental science and medicine. The journal accepts original and review articles as well as book reviews for publication. All the manuscripts are single-blind peer-reviewed for scientific quality and acceptance.
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