Mast cells in oral lichen planus and oral lichenoid lesions related to dental amalgam contact.

IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Brazilian oral research Pub Date : 2024-01-05 eCollection Date: 2024-01-01 DOI:10.1590/1807-3107bor-2024.vol38.0005
Mariana Saturnino de Noronha, Giovanna Ribeiro Souto, Fernanda Aragão Felix, Lucas Guimarães Abreu, Maria Cássia Ferreira Aguiar, Elismauro Francisco Mendonça, Ricardo Alves Mesquita
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Abstract

The aim of this study was to analyze the expression of mast cell markers toluidine blue, c-kit, and tryptase and presence of mononuclear inflammatory cells in oral lichen planus (OLP) and oral lichenoid lesions related to dental amalgam. Nineteen specimens of OLP, OLLC, and healthy oral mucosa were selected. Mononuclear inflammatory cells were analyzed. Histochemical and immunohistochemical analyses were performed using toluidine blue, anti-c-kit and anti-tryptase reagents, and the results were quantified in areas A and B of connective tissue. Mast cells of all OLP and OLLC samples were positive for toluidine blue, c-kit, and tryptase. The density of toluidine blue+, c-kit+ and tryptase+ mast cells was higher in tissue with OLP and OLLC compared with healthy controls (p < 0.05). No difference was noted in mast cells density between OLP and OLLC (p > 0.05). The density of tryptase+ mast cells was higher in the subepithelial region (area A) than the region below it (Area B) in OLLC (p = 0.047). The mononuclear inflammatory cell density was higher in OLLC compared to OLP, but without statistical significance (p > 0.05). A positive statistical correlation was found between mononuclear immune cells and density of c-kit+ and tryptase+ mast cells in OLP (r = 0.943 and r = 0.886, respectively). Our data demonstrate that the etiopathogenesis process of OLP and OLLC modulates the expansion and degranulation of mast cells; mast cells density, however, was similar between OLP and OLLC. The distribution of mast cells appears to vary along the lamina propria.

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与牙科汞合金接触有关的口腔扁平苔藓和口腔苔癣病变中的肥大细胞。
本研究旨在分析与牙科汞合金相关的口腔扁平苔藓(OLP)和口腔类苔藓病变中肥大细胞标志物甲苯胺蓝、c-kit和胰蛋白酶的表达以及单核炎症细胞的存在情况。研究人员选取了 19 份 OLP、OLLC 和健康口腔黏膜标本。对单核炎症细胞进行了分析。使用甲苯胺蓝、抗 kit 和抗色氨酸酶试剂进行组织化学和免疫组化分析,并对结缔组织 A 区和 B 区的结果进行量化。所有OLP和OLLC样本的肥大细胞均呈甲苯胺蓝、c-kit和胰蛋白酶阳性。与健康对照组相比,OLP和OLLC组织中甲苯胺蓝+、c-kit+和胰蛋白酶+肥大细胞的密度更高(p < 0.05)。OLP和OLLC的肥大细胞密度没有差异(p > 0.05)。OLLC患者上皮下区域(A区)的胰酶+肥大细胞密度高于其下方区域(B区)(p = 0.047)。OLLC的单核炎症细胞密度高于OLP,但无统计学意义(p > 0.05)。在OLP中,单核免疫细胞与c-kit+和胰蛋白酶+肥大细胞密度之间存在统计学上的正相关(r = 0.943和r = 0.886)。我们的数据表明,OLP 和 OLLC 的发病过程会调节肥大细胞的扩张和脱颗粒;但 OLP 和 OLLC 的肥大细胞密度相似。肥大细胞沿固有层的分布似乎有所不同。
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来源期刊
CiteScore
3.70
自引率
4.00%
发文量
107
审稿时长
12 weeks
期刊最新文献
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