Mast cells in oral lichen planus and oral lichenoid lesions related to dental amalgam contact.

IF 1.5 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Brazilian oral research Pub Date : 2024-01-05 eCollection Date: 2024-01-01 DOI:10.1590/1807-3107bor-2024.vol38.0005
Mariana Saturnino de Noronha, Giovanna Ribeiro Souto, Fernanda Aragão Felix, Lucas Guimarães Abreu, Maria Cássia Ferreira Aguiar, Elismauro Francisco Mendonça, Ricardo Alves Mesquita
{"title":"Mast cells in oral lichen planus and oral lichenoid lesions related to dental amalgam contact.","authors":"Mariana Saturnino de Noronha, Giovanna Ribeiro Souto, Fernanda Aragão Felix, Lucas Guimarães Abreu, Maria Cássia Ferreira Aguiar, Elismauro Francisco Mendonça, Ricardo Alves Mesquita","doi":"10.1590/1807-3107bor-2024.vol38.0005","DOIUrl":null,"url":null,"abstract":"<p><p>The aim of this study was to analyze the expression of mast cell markers toluidine blue, c-kit, and tryptase and presence of mononuclear inflammatory cells in oral lichen planus (OLP) and oral lichenoid lesions related to dental amalgam. Nineteen specimens of OLP, OLLC, and healthy oral mucosa were selected. Mononuclear inflammatory cells were analyzed. Histochemical and immunohistochemical analyses were performed using toluidine blue, anti-c-kit and anti-tryptase reagents, and the results were quantified in areas A and B of connective tissue. Mast cells of all OLP and OLLC samples were positive for toluidine blue, c-kit, and tryptase. The density of toluidine blue+, c-kit+ and tryptase+ mast cells was higher in tissue with OLP and OLLC compared with healthy controls (p < 0.05). No difference was noted in mast cells density between OLP and OLLC (p > 0.05). The density of tryptase+ mast cells was higher in the subepithelial region (area A) than the region below it (Area B) in OLLC (p = 0.047). The mononuclear inflammatory cell density was higher in OLLC compared to OLP, but without statistical significance (p > 0.05). A positive statistical correlation was found between mononuclear immune cells and density of c-kit+ and tryptase+ mast cells in OLP (r = 0.943 and r = 0.886, respectively). Our data demonstrate that the etiopathogenesis process of OLP and OLLC modulates the expansion and degranulation of mast cells; mast cells density, however, was similar between OLP and OLLC. The distribution of mast cells appears to vary along the lamina propria.</p>","PeriodicalId":9240,"journal":{"name":"Brazilian oral research","volume":"38 ","pages":"e005"},"PeriodicalIF":1.5000,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11376600/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian oral research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1590/1807-3107bor-2024.vol38.0005","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0

Abstract

The aim of this study was to analyze the expression of mast cell markers toluidine blue, c-kit, and tryptase and presence of mononuclear inflammatory cells in oral lichen planus (OLP) and oral lichenoid lesions related to dental amalgam. Nineteen specimens of OLP, OLLC, and healthy oral mucosa were selected. Mononuclear inflammatory cells were analyzed. Histochemical and immunohistochemical analyses were performed using toluidine blue, anti-c-kit and anti-tryptase reagents, and the results were quantified in areas A and B of connective tissue. Mast cells of all OLP and OLLC samples were positive for toluidine blue, c-kit, and tryptase. The density of toluidine blue+, c-kit+ and tryptase+ mast cells was higher in tissue with OLP and OLLC compared with healthy controls (p < 0.05). No difference was noted in mast cells density between OLP and OLLC (p > 0.05). The density of tryptase+ mast cells was higher in the subepithelial region (area A) than the region below it (Area B) in OLLC (p = 0.047). The mononuclear inflammatory cell density was higher in OLLC compared to OLP, but without statistical significance (p > 0.05). A positive statistical correlation was found between mononuclear immune cells and density of c-kit+ and tryptase+ mast cells in OLP (r = 0.943 and r = 0.886, respectively). Our data demonstrate that the etiopathogenesis process of OLP and OLLC modulates the expansion and degranulation of mast cells; mast cells density, however, was similar between OLP and OLLC. The distribution of mast cells appears to vary along the lamina propria.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
与牙科汞合金接触有关的口腔扁平苔藓和口腔苔癣病变中的肥大细胞。
本研究旨在分析与牙科汞合金相关的口腔扁平苔藓(OLP)和口腔类苔藓病变中肥大细胞标志物甲苯胺蓝、c-kit和胰蛋白酶的表达以及单核炎症细胞的存在情况。研究人员选取了 19 份 OLP、OLLC 和健康口腔黏膜标本。对单核炎症细胞进行了分析。使用甲苯胺蓝、抗 kit 和抗色氨酸酶试剂进行组织化学和免疫组化分析,并对结缔组织 A 区和 B 区的结果进行量化。所有OLP和OLLC样本的肥大细胞均呈甲苯胺蓝、c-kit和胰蛋白酶阳性。与健康对照组相比,OLP和OLLC组织中甲苯胺蓝+、c-kit+和胰蛋白酶+肥大细胞的密度更高(p < 0.05)。OLP和OLLC的肥大细胞密度没有差异(p > 0.05)。OLLC患者上皮下区域(A区)的胰酶+肥大细胞密度高于其下方区域(B区)(p = 0.047)。OLLC的单核炎症细胞密度高于OLP,但无统计学意义(p > 0.05)。在OLP中,单核免疫细胞与c-kit+和胰蛋白酶+肥大细胞密度之间存在统计学上的正相关(r = 0.943和r = 0.886)。我们的数据表明,OLP 和 OLLC 的发病过程会调节肥大细胞的扩张和脱颗粒;但 OLP 和 OLLC 的肥大细胞密度相似。肥大细胞沿固有层的分布似乎有所不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
3.70
自引率
4.00%
发文量
107
审稿时长
12 weeks
期刊最新文献
Assessment of Brazilian hospital and healthcare service infrastructure for cleft lip and palate patients. Comparison of the analgesic efficacy of spray and tablet flurbiprofen for pain after soft tissue surgery. Efficacy of xenogeneic collagen matrix in the treatment of gingival recessions: a controlled clinical trial. Facial morphology analysis of Caucasian Brazilian adult women using stereophotogrammetry. Impact of an educational intervention regarding tobacco counseling on dentists and dental students.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1