Deubiquitylase USP31 Induces Autophagy and Promotes the Progression in Lung Squamous Cell Carcinoma Cells by Stabilizing E2F1 Expression.

IF 3.5 4区医学 Q3 ONCOLOGY Current cancer drug targets Pub Date : 2024-01-01 DOI:10.2174/0115680096264557231124102054

Wenjun Liang, Mingxia Yang, Xiaohua Wang, Yan Qian, Ruichen Gao, Yujia Shi, Xuejun Shi, Lei Shi, Ting Xu, Qian Zhang

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Abstract

Background: Autophagy exerts a vital role in the progression of lung squamous cell carcinoma (LUSC). Ubiquitin-specific peptidase 31 (USP31) has recently been found to be involved in the development of a variety of cancers. However, whether USP31 modulates autophagy in LUSC remains unclear.

Methods: This study revealed that high levels of USP31 were discovered in LUSC tissue samples employing the Gene Expression Profiling Interactive Analysis (GEPIA) database, quantitative real- time PCR (qRT-PCR), and Western blot analysis. Cell proliferation was tested via cell counting kit 8 (CCK-8) as well as colony formation, demonstrating that USP31-stable knockdown reduced cell viability.

Results: Immunofluorescence analysis illustrated that USP31 knockdown blocked the occurrence of LUSC autophagy. Meanwhile, USP31 has been shown to stabilize the expression of E2F transcription factor 1 (E2F1) through the proteasome pathway. Furthermore, overexpressed E2F1 effectively eliminated the effect of USP31 knockdown on LUSC cell proliferation and autophagy.

Conclusion: In summary, this investigation proved that USP31 promoted LUSC cell growth and autophagy, at least in part by stabilizing E2F1 expression, which provided a potential therapeutic gene for the treatment of LUSC.

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去泛素化酶 USP31 通过稳定 E2F1 的表达诱导自噬并促进肺鳞状细胞癌细胞的进展

背景自噬在肺鳞癌（LUSC）的进展过程中发挥着至关重要的作用。最近发现泛素特异性肽酶 31（USP31）与多种癌症的发展有关。然而，USP31 是否会调节 LUSC 中的自噬作用仍不清楚：本研究利用基因表达谱交互式分析（GEPIA）数据库、定量实时 PCR（qRT-PCR）和 Western 印迹分析发现，在 LUSC 组织样本中发现了高水平的 USP31。通过细胞计数试剂盒 8（CCK-8）检测细胞增殖以及集落形成，证明 USP31 稳定敲除会降低细胞活力：结果：免疫荧光分析表明，敲除 USP31 会阻止 LUSC 自噬的发生。同时，USP31 可通过蛋白酶体途径稳定 E2F 转录因子 1（E2F1）的表达。此外，过表达的 E2F1 能有效消除 USP31 敲除对 LUSC 细胞增殖和自噬的影响：总之，本研究证明 USP31 至少部分通过稳定 E2F1 的表达促进了 LUSC 细胞的生长和自噬，为治疗 LUSC 提供了潜在的治疗基因。

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来源期刊

Current cancer drug targets 医学-肿瘤学

CiteScore

5.40

自引率

0.00%

发文量

105

审稿时长

1 months

期刊介绍： Current Cancer Drug Targets aims to cover all the latest and outstanding developments on the medicinal chemistry, pharmacology, molecular biology, genomics and biochemistry of contemporary molecular drug targets involved in cancer, e.g. disease specific proteins, receptors, enzymes and genes. Current Cancer Drug Targets publishes original research articles, letters, reviews / mini-reviews, drug clinical trial studies and guest edited thematic issues written by leaders in the field covering a range of current topics on drug targets involved in cancer. As the discovery, identification, characterization and validation of novel human drug targets for anti-cancer drug discovery continues to grow; this journal has become essential reading for all pharmaceutical scientists involved in drug discovery and development.