Behrang Sarshari, Raziyeh Zareh-Khoshchehreh, Mehdi Tavallaei, Mohsen Rajabnia, Mohammad Tayefeh Norooz, S. Mohebbi, M. Ravanshad
{"title":"Comparative and phylogenetic analysis of Epstein–Barr virus isolates in gastric cancer and chronic gastritis patients in Iran","authors":"Behrang Sarshari, Raziyeh Zareh-Khoshchehreh, Mehdi Tavallaei, Mohsen Rajabnia, Mohammad Tayefeh Norooz, S. Mohebbi, M. Ravanshad","doi":"10.2217/fvl-2023-0144","DOIUrl":null,"url":null,"abstract":"Aim: To investigate the correlation between Epstein–Barr virus (EBV) variants and EBV-associated gastric cancer (EBVaGC) in Iran. Materials & methods: Biopsies from 21 gastric cancer (4 positive EBER-ISH (EBVaGC) and 17 negative EBER-ISH) and 11 gastritis patients were analyzed using nested PCR and sequencing. Results: Genotype 1 (75%) was the predominant EBV genotype. The P-thr variant was the most common in all groups (positive EBER-ISH 75%, negative EBER-ISH 76.4%, and gastritis 81.8%), followed by P-ala. Additionally, the predominant LMP1 strain in all groups was Med. Sequencing analysis revealed a strong correlation between the P-thr and type 1. Conclusion: Since no preferential correlations were observed between distinct EBV variants and the study groups, these results might indicate geographic polymorphisms.","PeriodicalId":503758,"journal":{"name":"Future Virology","volume":"88 22","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future Virology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2217/fvl-2023-0144","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Aim: To investigate the correlation between Epstein–Barr virus (EBV) variants and EBV-associated gastric cancer (EBVaGC) in Iran. Materials & methods: Biopsies from 21 gastric cancer (4 positive EBER-ISH (EBVaGC) and 17 negative EBER-ISH) and 11 gastritis patients were analyzed using nested PCR and sequencing. Results: Genotype 1 (75%) was the predominant EBV genotype. The P-thr variant was the most common in all groups (positive EBER-ISH 75%, negative EBER-ISH 76.4%, and gastritis 81.8%), followed by P-ala. Additionally, the predominant LMP1 strain in all groups was Med. Sequencing analysis revealed a strong correlation between the P-thr and type 1. Conclusion: Since no preferential correlations were observed between distinct EBV variants and the study groups, these results might indicate geographic polymorphisms.
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