Building a degradable scaffold with 3D printing using Masquelet technique to promote osteoblast differentiation and angiogenesis in chronic tibial osteomyelitis with bone defects

IF 6.8 3区 医学 Q1 ENGINEERING, BIOMEDICAL International Journal of Bioprinting Pub Date : 2024-01-09 DOI:10.36922/ijb.1461
Fan Liu, Chaohan Wu, Xinhui Wang, Rong-Zuo Guo, Tianhua Dong, Tao Zhang
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Abstract

The aim of this study was to investigate the use of three-dimensional (3D) printing technology to create a biodegradable scaffold loaded with WNT5A protein and assess its impact on chronic tibial osteomyelitis with bone defects (CTO&BD), focusing on osteoblast differentiation and angiogenesis. We extracted RNA from peripheral blood of healthy individuals and CTO&BD patients for sequencing, followed by differential expression and functional enrichment analysis. Network analysis was performed to identify core genes associated with CTO&BD and construct a protein–protein interaction network. Using Masquelet technique, we fabricated a 3D-printed biodegradable scaffold (G40T60@WNT5A) and conducted various experiments, including rheological testing, printability evaluation, Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy analysis, as well as mechanical and degradation performance assessments. In in vivo experiments, we observed the formation of induced membranes in a CTO&BD rat model implanted with the scaffold. In vitro experiments involved the assessment of scaffold toxicity on rat bone marrow mesenchymal stem cells and umbilical vein endothelial cells, as well as the influence on osteoblast differentiation and angiogenesis. Molecular biology techniques were used to analyze gene and protein expression levels. We discovered for the first time that WNT5A may play a crucial role in CTO&BD. The biodegradable scaffold prepared by 3D printing (G40T60@WNT5A) exhibited excellent biocompatibility in vitro. This scaffold significantly promoted the formation of induced membranes in CTO&BD rats and further enhanced osteoblast differentiation and angiogenesis. In conclusion, this study utilized innovative 3D printing technology to fabricate the G40T60@WNT5A scaffold, confirming its potential application in the treatment of CTO&BD, particularly in promoting osteoblast differentiation and angiogenesis. This research provides new methods and theoretical support for the treatment of bone defects.
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利用 Masquelet 技术通过 3D 打印技术构建可降解支架,促进慢性胫骨骨髓炎伴骨缺损患者的成骨细胞分化和血管生成
本研究的目的是研究如何利用三维(3D)打印技术制造出负载有 WNT5A 蛋白的可生物降解支架,并评估其对慢性胫骨骨髓炎伴骨缺损(CTO&BD)的影响,重点关注成骨细胞分化和血管生成。我们从健康人和 CTO&BD 患者的外周血中提取 RNA 进行测序,然后进行差异表达和功能富集分析。通过网络分析,我们确定了与 CTO&BD 相关的核心基因,并构建了蛋白-蛋白相互作用网络。我们利用 Masquelet 技术制作了 3D 打印生物可降解支架(G40T60@WNT5A),并进行了各种实验,包括流变学测试、可印刷性评估、傅立叶变换红外光谱、X 射线衍射、扫描电子显微镜分析以及机械和降解性能评估。在体内实验中,我们在植入该支架的 CTO&BD 大鼠模型中观察到了诱导膜的形成。体外实验包括评估支架对大鼠骨髓间充质干细胞和脐静脉内皮细胞的毒性,以及对成骨细胞分化和血管生成的影响。我们利用分子生物学技术分析了基因和蛋白质的表达水平。我们首次发现 WNT5A 可能在 CTO&BD 中发挥关键作用。通过三维打印制备的生物可降解支架(G40T60@WNT5A)在体外表现出良好的生物相容性。这种支架能明显促进 CTO&BD 大鼠诱导膜的形成,并进一步促进成骨细胞分化和血管生成。总之,本研究利用创新的 3D 打印技术制造了 G40T60@WNT5A 支架,证实了其在治疗 CTO&BD 方面的潜在应用,尤其是在促进成骨细胞分化和血管生成方面。这项研究为骨缺损的治疗提供了新的方法和理论支持。
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来源期刊
CiteScore
6.90
自引率
4.80%
发文量
81
期刊介绍: The International Journal of Bioprinting is a globally recognized publication that focuses on the advancements, scientific discoveries, and practical implementations of Bioprinting. Bioprinting, in simple terms, involves the utilization of 3D printing technology and materials that contain living cells or biological components to fabricate tissues or other biotechnological products. Our journal encompasses interdisciplinary research that spans across technology, science, and clinical applications within the expansive realm of Bioprinting.
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