In vitro assessment of Momordica charantia/Hypericum perforatum oils loaded PCL/Collagen fibers: Novel scaffold for tissue engineering.

IF 3.1 4区 医学 Q2 BIOPHYSICS Journal of Applied Biomaterials & Functional Materials Pub Date : 2024-01-01 DOI:10.1177/22808000231221067
Emre Fatih Ediz, Cansu Güneş, Meltem Demirel Kars, Ahmet Avcı
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Abstract

The research on tissue engineering applications has been progressing to manufacture ideal tissue scaffold biomaterials. In this study, a double-layered electrospun biofiber scaffold biomaterial including Polycaprolactone (PCL)/Collagen (COL) fibrous inner layer and PCL/ Momordica charantia (MC) and Hypericum perforatum (HP) oils fibrous outer layer was developed to manufacture a functional, novel tissue scaffold with the advantageous mechanical and biological properties. The main approach was to combine the natural perspective using medicinal oils with an engineering point of view to fabricate a potential functional scaffold for tissue engineering. Medicinal plants MC and HP are rich in functional oils and incorporation of them in a tissue scaffold will unveil their potential to augment both new tissue formation and wound healing. In this study, a novel double-layered scaffold prototype was fabricated using electrospinning technique with two PCL fiber layers, first is composed of collagen, and second is composed of oils extracted from medicinal plants. Initially, the composition of plant oils was analyzed. Thereafter the biofiber scaffold layers were fabricated and were evaluated in terms of morphology, physicochemistry, thermal and mechanical features, wettability, in vitro bio-degradability. Double-layered scaffold prototype was further analyzed in terms of in vitro biocompatibility and antibacterial effect. The medicinal oils blend provided antioxidant and antibacterial properties to the novel PCL/Oils layer. The results signify that inner PCL/COL layer exhibited advanced biodegradability of 8.5% compared to PCL and enhanced wettability with 11.7° contact angle. Strength of scaffold prototype was 5.98 N/mm2 thanks to the elastic PCL fibrous matrix. The double-layered functional biofiber scaffold enabled 92% viability after 72 h contact with fibroblast cells and furthermore provided feasible attachment sites for the cells. The functional scaffold prototype's noteworthy mechanical, chemical, and biological features enable it to be suggested as a different novel biomaterial with the potential to be utilized in tissue engineering applications.

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对负载 PCL/胶原纤维的 Momordica charantia/Hypericum perforatum 油进行体外评估:用于组织工程的新型支架
为制造理想的组织支架生物材料,有关组织工程应用的研究一直在取得进展。本研究开发了一种双层电纺生物纤维支架生物材料,包括聚己内酯(PCL)/胶原蛋白(COL)纤维内层和 PCL/ Momordica charantia(MC)和金丝桃油(HP)纤维外层,以制造一种具有良好机械和生物特性的功能性新型组织支架。主要方法是将药用油脂的自然观点与工程观点相结合,为组织工程制造一种潜在的功能性支架。药用植物 MC 和 HP 含有丰富的功能性油脂,将它们融入组织支架中将会发现它们在促进新组织形成和伤口愈合方面的潜力。本研究利用电纺丝技术制造了一种新型双层支架原型,其中有两层 PCL 纤维,第一层由胶原蛋白组成,第二层由从药用植物中提取的油组成。首先分析了植物油的成分。之后,制作了生物纤维支架层,并从形态、物理化学、热和机械特性、润湿性、体外生物降解性等方面进行了评估。还进一步分析了双层支架原型的体外生物相容性和抗菌效果。药用油混合物为新型 PCL/油层提供了抗氧化和抗菌特性。结果表明,与 PCL 相比,PCL/COL 内层的生物降解性提高了 8.5%,润湿性增强,接触角为 11.7°。由于 PCL 纤维基质具有弹性,支架原型的强度为 5.98 N/mm2。在与成纤维细胞接触 72 小时后,双层功能生物纤维支架的存活率达到 92%,而且还为细胞提供了可行的附着点。该功能性支架原型具有显著的机械、化学和生物特性,因此被认为是一种新型生物材料,有望应用于组织工程领域。
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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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