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Vanillin loaded-physically crosslinked PVA/chitosan/itaconic membranes for topical wound healing applications 用于局部伤口愈合的香兰素负载型物理交联 PVA/壳聚糖/吐温膜
IF 2.5 4区 医学 Q2 BIOPHYSICS Pub Date : 2024-09-19 DOI: 10.1177/22808000241281273
El-Refaie Kenawy, Elbadawy A Kamoun, Samia M Elsigeny, Samira Heikal, Ashraf A El-Shehawy, Yehia A-G Mahmoud
Vanillin loaded-physically crosslinked hydrogel membranes made of PVA/chitosan/itaconic acid (PVA-CS-IA) were prepared using freezing-thawing (F-T) cycle method. To ensure the entanglement of PVA-CS-IA chains, three F-T cycles were repeated. The polymeric chains entanglements were confirmed and characterized by different instrumental characterizations. Physicochemical properties for example, swelling ratio, mechanical characteristics, gel fraction percentage (GF%), hydrolytic degradation, and thermal stability of PVA-CS-IA membrane were discussed in detail. The findings showed that the swelling ratio, mechanical characteristics, and hydrolytic degradation of the crosslinked membranes enhanced with increasing CS-IA contents in membranes composition; however, GF% gradually declined with CS-IA content. Additionally, cell viability test using HFB-4 cell line and antimicrobial activity against Staphylococcus aureus and Escherichia coli were evaluated using MTT assay and the bacterium growth inhibition percentage method; respectively. Notably, with varying incubation durations and membrane concentrations, all examined constructed hydrogels showed significant cell survival percentages. The findings supported the notion that produced hydrogel membranes might be used in a professional setting as antibacterial dressings or biomaterials for quick wound healing rate.
采用冷冻-解冻(F-T)循环法制备了由 PVA/壳聚糖/衣康酸(PVA-CS-IA)制成的香兰素负载物理交联水凝胶膜。为确保 PVA-CS-IA 链的缠结,需要重复三次 F-T 循环。聚合物链的缠结通过不同的仪器表征进行了确认和表征。详细讨论了 PVA-CS-IA 膜的物理化学特性,如溶胀率、机械特性、凝胶分数百分比(GF%)、水解降解和热稳定性。研究结果表明,随着膜成分中 CS-IA 含量的增加,交联膜的溶胀率、机械特性和水解降解率都有所提高;但凝胶分数百分比随 CS-IA 含量的增加而逐渐降低。此外,还使用 MTT 法和细菌生长抑制百分率法分别评估了 HFB-4 细胞系的细胞活力测试以及对金黄色葡萄球菌和大肠杆菌的抗菌活性。值得注意的是,在不同的培养时间和膜浓度下,所有检测的水凝胶都显示出显著的细胞存活率。研究结果支持了这一观点,即生产的水凝胶膜可用作专业抗菌敷料或生物材料,以加快伤口愈合速度。
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引用次数: 0
Physicomechanical, morphological and tribo-deformation characteristics of lightweight WC/AZ31B Mg-matrix biocomposites for hip joint applications 用于髋关节的轻质 WC/AZ31B 镁基质生物复合材料的物理力学、形态学和三重变形特性
IF 2.5 4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-05-04 DOI: 10.1177/22808000231214359
Nandhakumar Dhandapani, A Bovas Herbert Bejaxhin, Gajendran Periyaswamy, Narayan Ramanan, Jayaraman Arunprasad, Sivanraju Rajkumar, Shubham Sharma, Gurminder Singh, Fuad A Awwad, M Ijaz Khan, Emad AA Ismail
Exploring high strength materials with a higher concentration of reinforcements in the alloy proves to be a challenging task. This research has explored magnesium-based composites (AZ31B alloy) with tungsten carbide reinforcements, enhancing strength for medical joint replacements via league championship optimisation. The primary objective is to enhance medical joint replacement biomaterials employing magnesium-based composites, emphasising the AZ31B alloy with tungsten carbide reinforcements. The stir casting method is utilised in the manufacture of magnesium matrix composites (MMCs), including varied percentages of tungsten carbide (WC). The mechanical characteristics, such as micro-hardness, tensile strength, and yield strength, have been assessed and compared with computational simulations. The wear studies have been carried out to analyse the tribological behaviour of the composites. Additionally, this study investigates the prediction of stress and the distribution of forces inside bone and joint structures, therefore offering significant contributions to the field of biomedical research. This research contemplates the use of magnesium-based MMCs for the discovery of biomaterials suitable for medical joint replacement. The study focuses on the magnesium alloy AZ31B, with particles ranging in size from 40 to 60 microns used as the matrix material. Moreover, the outcomes have revealed that when combined with MMCs based on AZ31B-magnesium matrix, the WC particle emerges as highly effective reinforcements for the fabrication of lightweight, high-strength biomedical composites. This study uses the league championship optimisation (LCO) approach to identify critical variables impacting the synthesis of Mg MMCs from an AZ31B-based magnesium alloy. The scanning electron microscopy (SEM) images are meticulously analysed to depict the dispersion of WC particulates and the interface among the magnesium (Mg) matrix and WC reinforcement. The SEM analysis has explored the mechanisms underlying particle pull-out, the characteristics of inter-particle zones, and the influence of the AZ31B matrix on the enhancement of the mechanical characteristics of the composites. The application of finite element analysis (FEA) is being used in order to make predictions regarding the distribution of stress and the interactions of forces within the model of the hip joint. This study has compared the physico-mechanical and tribological characteristics of WC to distinct combinations of 0%, 5%, 10% and 15%, and its impact on the performance improvements. SEM analysis has confirmed the findings’ improved strength and hardness, particularly when 10%–15% of WC was incorporated. Following the incorporation of 10% of WC particles within Mg-alloy matrix, the outcomes of the study has exhibited enhanced strength and hardness, which furthermore has been evident by utilising SEM analysis. Using ANSYS, structural deformation and stress levels are predicted, along with strength charac
事实证明,探索合金中强化剂浓度更高的高强度材料是一项具有挑战性的任务。本研究探索了含有碳化钨增强材料的镁基复合材料(AZ31B 合金),通过联盟冠军优化提高医用关节置换材料的强度。研究的主要目的是利用镁基复合材料增强医用关节置换生物材料的强度,重点是带有碳化钨增强材料的 AZ31B 合金。镁基复合材料(MMC)的制造采用搅拌铸造法,其中包括不同比例的碳化钨(WC)。对微观硬度、拉伸强度和屈服强度等机械特性进行了评估,并与计算模拟进行了比较。此外,还进行了磨损研究,以分析复合材料的摩擦学行为。此外,这项研究还对骨骼和关节结构内部的应力和力分布进行了预测,从而为生物医学研究领域做出了重大贡献。本研究考虑使用镁基 MMC 来发现适用于医用关节置换的生物材料。研究的重点是镁合金 AZ31B,基体材料的颗粒大小从 40 微米到 60 微米不等。此外,研究结果表明,当与基于 AZ31B 镁基质的 MMC 相结合时,WC 颗粒成为制造轻质、高强度生物医学复合材料的高效增强材料。本研究采用联盟冠军优化(LCO)方法来确定影响从基于 AZ31B 的镁合金合成镁 MMC 的关键变量。对扫描电子显微镜(SEM)图像进行了细致分析,以描述碳化钨颗粒的分散情况以及镁(Mg)基体和碳化钨增强体之间的界面。扫描电子显微镜分析探讨了颗粒拉出的内在机制、颗粒间区域的特征以及 AZ31B 基体对增强复合材料机械特性的影响。有限元分析(FEA)的应用是为了预测髋关节模型中的应力分布和力的相互作用。这项研究比较了 0%、5%、10% 和 15% WC 不同组合的物理机械和摩擦学特性及其对性能改善的影响。扫描电子显微镜分析证实了研究结果对强度和硬度的改善作用,尤其是在添加 10%-15%的 WC 时。在镁合金基体中加入 10% 的碳化钨颗粒后,研究结果表明强度和硬度都得到了提高,这一点在 SEM 分析中也得到了证实。使用 ANSYS 预测了结构变形和应力水平,以及强度特性,如硬度增加到 71 HRC,抗拉强度达到 140-150 兆帕,屈服强度接近 100-110 兆帕。模拟结果有助于深入了解接头在各种加载条件下的行为,从而增强了这项研究在生物医学环境中的意义。
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引用次数: 0
Effects of different antiviral mouthwashes on the surface roughness, hardness, and color stability of composite CAD/CAM materials 不同抗病毒漱口水对 CAD/CAM 复合材料表面粗糙度、硬度和颜色稳定性的影响
IF 2.5 4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-04-28 DOI: 10.1177/22808000241248886
Ahmet Hazar, Ecehan Hazar
Objective:To evaluate the effect of COVID-19 preventive mouthwashes on the surface hardness, surface roughness (Ra), and color change (ΔE) of three different polymer-based composite CAD/CAM materials (Vita Enamic (ENA), Grandio Block (GB), Lava Ultimate (LU)).Methods:A total of 100 rectangular-shaped specimens with dimensions of 2 mm × 7 mm × 12 mm were obtained by sectioning three different CAD/CAM blocks and randomly divided into five subgroups according to the 30 days of mouthwash immersion protocol as follows: Control: artificial saliva, PVP-I: 1% povidone-iodine, HP: 1.5% hydrogen peroxide, CPC: mouthwash containing 0.075% cetylpyridinium chloride, EO: mouthwash containing essential oils. Microhardness, Ra, and ΔE values were measured at baseline and after 30 days of immersion protocols. Data were analyzed using the Wald Chi-square, two-way ANOVA, and post hoc Tukey tests.Results:The independent factors (materials and solutions) significantly influenced the microhardness and color ( p < 0.001). Ra of the materials was not affected by any of the mouthwashes ( p > 0.05). The microhardness and color of each material varied significantly after immersion in PvP-I and HP ( p < 0.05). The highest percentage change in microhardness, Ra, and ΔE was found in LU immersed in PvP-I and HP mouthwashes, while the lowest change was found in ENA groups ( p < 0.05).Conclusion:Within the limitations of this study, it was found that the surface hardness and color of tested polymer-based composite CAD/CAM materials are susceptible to degradation and change after 30 days of immersion in 1% PvP-I and 1.5% HP mouthwashes.
目的:评估 COVID-19 预防性漱口水对三种不同聚合物基复合 CAD/CAM 材料(Vita Enamic (ENA)、Grandio Block (GB)、Lava Ultimate (LU))的表面硬度、表面粗糙度 (Ra) 和颜色变化 (ΔE)的影响。方法:通过对三种不同的 CAD/CAM 块进行切片,共获得 100 个尺寸为 2 mm × 7 mm × 12 mm 的矩形试样,并按照漱口水浸泡 30 天的方案随机分为以下 5 个亚组:对照组:人工唾液;PVP-I:1% 聚维酮碘;HP:1.5% 过氧化氢;CPC:含 0.075% 氯化十六烷基吡啶的漱口水;EO:含精油的漱口水。微硬度、Ra 和 ΔE 值分别在基线和浸泡 30 天后测量。结果:独立因素(材料和溶液)对显微硬度和颜色有显著影响(p < 0.001)。材料的 Ra 值不受任何漱口水的影响(p > 0.05)。在 PvP-I 和 HP 中浸泡后,每种材料的显微硬度和颜色都有显著变化(p < 0.05)。结论:在本研究的局限性范围内,研究发现在 1%的 PvP-I 和 1.5% 的 HP 漱口水中浸泡 30 天后,测试的聚合物基复合 CAD/CAM 材料的表面硬度和颜色容易发生降解和变化。
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引用次数: 0
Retraction Notice: "Comparative evaluation of Sapindus Mukorossi extract with 17% EDTA on smear layer removal in various parts of the tooth root". 撤稿通知:"无患子提取物与 17%乙二胺四乙酸对牙根不同部位涂抹层去除效果的比较评估"。
IF 2.5 4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-01-01 DOI: 10.1177/22808000241227499
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引用次数: 0
The injectable hydrogel loading cannabidiol to regulate macrophage polarization in vitro for the treatment of chronic enteritis. 装载大麻二酚的可注射水凝胶可在体外调节巨噬细胞极化,用于治疗慢性肠炎。
IF 3.1 4区 医学 Q2 BIOPHYSICS Pub Date : 2024-01-01 DOI: 10.1177/22808000241289022
Ye Wang, Xingming Ji, Xinyi Wang, Mengyu Sun, Cheng Li, Dongmei Wu

Objective: Chronic bowel disease has the characteristics of high recurrence rate, prolonged and non-healing, and the incidence has increased year by year in recent years. Cannabidiol (CBD) has significant anti-inflammatory and antioxidant activities, but it is limited by its characteristics of fat solubility and low bioavailability. This study aims to treat chronic inflammatory bowel disease by preparing a CBD-loaded hydrogel system (GelMA + CBD) that can deliver CBD in situ and improve its bioavailability through slow release.

Method: The study designed and constructed GelMA + CBD, and its surface morphology was observed by scanning electron microscopy, and its pore size, swelling rate and release rate were evaluated to evaluate its bioactivity and biosafety. The expression of various inflammatory factors was detected by ELISA, and the expression of protein and reactive oxygen species were observed by laser confocal microscopy to evaluate their anti-inflammatory and antioxidant properties.

Results: Our study found that GelMA + CBD with biosafety, could make CBD be slowly released, and effectively inhibit the M1-type polarization of macrophages in vitro, and promote the M2-type polarization. In addition, GelMA + CBD can also reduce the expression of pro-inflammatory factors (such as iNOS) in macrophages, and increase the expression of anti-inflammatory factors (such as Arg-1), clear intracellular reactive oxygen species (ROS), and relieve oxidative stress.

Conclusion: The vitro experiments have confirmed that the CBD-loaded hydrogel system has good biosafety, and can alleviate inflammation by regulating the polarization direction of macrophages, and then inhibiting the secretion of pro-inflammatory factors, laying a strong foundation for the treatment of chronic enteritis.

目的:慢性肠病具有复发率高、病程长、久治不愈等特点,近年来发病率逐年上升。大麻二酚(CBD)具有显著的抗炎和抗氧化活性,但受限于其脂溶性和低生物利用度的特点。本研究旨在通过制备一种负载CBD的水凝胶系统(GelMA + CBD)来治疗慢性炎症性肠病:本研究设计并构建了GelMA + CBD,通过扫描电镜观察了其表面形态,并对其孔径、膨胀率和释放率进行了评估,以评价其生物活性和生物安全性。用酶联免疫吸附法检测各种炎症因子的表达,用激光共聚焦显微镜观察蛋白质和活性氧的表达,以评价其抗炎和抗氧化特性:研究发现,GelMA+CBD具有生物安全性,能使CBD缓慢释放,有效抑制体外巨噬细胞的M1型极化,促进M2型极化。此外,GelMA + CBD 还能减少巨噬细胞中促炎因子(如 iNOS)的表达,增加抗炎因子(如 Arg-1)的表达,清除细胞内活性氧(ROS),缓解氧化应激:体外实验证实,CBD负载水凝胶系统具有良好的生物安全性,可通过调节巨噬细胞的极化方向,进而抑制促炎因子的分泌,缓解炎症反应,为慢性肠炎的治疗奠定了坚实的基础。
{"title":"The injectable hydrogel loading cannabidiol to regulate macrophage polarization in vitro for the treatment of chronic enteritis.","authors":"Ye Wang, Xingming Ji, Xinyi Wang, Mengyu Sun, Cheng Li, Dongmei Wu","doi":"10.1177/22808000241289022","DOIUrl":"https://doi.org/10.1177/22808000241289022","url":null,"abstract":"<p><strong>Objective: </strong>Chronic bowel disease has the characteristics of high recurrence rate, prolonged and non-healing, and the incidence has increased year by year in recent years. Cannabidiol (CBD) has significant anti-inflammatory and antioxidant activities, but it is limited by its characteristics of fat solubility and low bioavailability. This study aims to treat chronic inflammatory bowel disease by preparing a CBD-loaded hydrogel system (GelMA + CBD) that can deliver CBD in situ and improve its bioavailability through slow release.</p><p><strong>Method: </strong>The study designed and constructed GelMA + CBD, and its surface morphology was observed by scanning electron microscopy, and its pore size, swelling rate and release rate were evaluated to evaluate its bioactivity and biosafety. The expression of various inflammatory factors was detected by ELISA, and the expression of protein and reactive oxygen species were observed by laser confocal microscopy to evaluate their anti-inflammatory and antioxidant properties.</p><p><strong>Results: </strong>Our study found that GelMA + CBD with biosafety, could make CBD be slowly released, and effectively inhibit the M1-type polarization of macrophages in vitro, and promote the M2-type polarization. In addition, GelMA + CBD can also reduce the expression of pro-inflammatory factors (such as iNOS) in macrophages, and increase the expression of anti-inflammatory factors (such as Arg-1), clear intracellular reactive oxygen species (ROS), and relieve oxidative stress.</p><p><strong>Conclusion: </strong>The vitro experiments have confirmed that the CBD-loaded hydrogel system has good biosafety, and can alleviate inflammation by regulating the polarization direction of macrophages, and then inhibiting the secretion of pro-inflammatory factors, laying a strong foundation for the treatment of chronic enteritis.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial and antitumor activities of an alginate-based membrane loaded with bismuth nanoparticles and cetylpyridinium chloride. 负载纳米铋颗粒和氯化十六烷基吡啶的藻酸盐膜的抗菌和抗肿瘤活性。
IF 2.5 4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-01-01 DOI: 10.1177/22808000241236590
Claudio Cabral-Romero, Rene Hernández-Delgadillo, Sergio Eduardo Nakagoshi-Cepeda, Rosa Isela Sánchez-Najéra, Erandi Escamilla-García, Juan Manuel Solís-Soto, Claudia María García-Cuellar, Yesennia Sánchez-Pérez, Samantha Maribel Flores-Treviño, Nayely Pineda-Aguilar, Juan Valerio Cauich-Rodríguez, Irene Meester, Shankararaman Chellam

Objective: To evaluate the antitumor and antimicrobial properties of an alginate-based membrane (ABM) loaded with bismuth lipophilic nanoparticles (BisBAL NPs) and cetylpyridinium chloride (CPC) on clinically isolated bacteria and a pancreatic cancer cell line.

Material and methods: The BisBAL NP-CPC ABM was characterized using optical and scanning electron microscopy (SEM). The antimicrobial potential was measured using the disk-diffusion assay, and antibiofilm activity was determined through the live/dead assay and fluorescence microscopy. The antitumor activity was analyzed on the pancreatic cell line (Panc 03.27) using the MTT assay and live/dead assay with fluorescence microscopy.

Results: After a 24-h exposure (37°C, aerobic conditions), 5 µM BisBAL NP reduced the growth of K. pneumoniae by 77.9%, while 2.5 µM BisBAL NP inhibited the growth of Salmonella, E. faecalis and E. faecium by 82.9%, 82.6%, and 78%, respectively (p < 0.0001). The BisBAL NPs-CPC ABM (at a ratio of 10:1; 500 and 50 µM, respectively) inhibited the growth of all isolated bacteria, producing inhibition halos of 9.5, 11.2, 7, and 10.3 mm for K. pneumoniae, Salmonella, E. faecalis, and E. faecium, respectively, in contrast to the 6.5, 9.5, 8.5, and 9.8 mm obtained with 100 µM ceftriaxone (p < 0.0001). The BisBAL NPs-CPC ABM also reduced bacterial biofilms, with 81.4%, 74.5%, 97.1%, and 79.5% inhibition for K. pneumoniae, E. faecium, E. faecalis, and Salmonella, respectively. Furthermore, the BisBAL NPs-CPC ABM decreased Panc 03.27 cell growth by 76%, compared to 18% for drug-free ABM. GEM-ABM reduced tumoral growth by 73%. The live/dead assay confirmed that BisBAL NPs-CPC-ABM and GEM-ABM were cytotoxic for the turmoral Panc 03.27 cells.

Conclusion: An alginate-based membrane loaded with BisBAL NP and CPC exhibits dual antimicrobial and antitumoral efficacy. Therefore, it could be applied in cancer treatment and to diminish the occurrence of surgical site infections.

目的评估负载有亲脂性纳米粒子铋(BisBAL NPs)和氯化十六烷基吡啶(CPC)的藻酸盐基膜(ABM)对临床分离细菌和胰腺癌细胞系的抗肿瘤和抗菌特性:使用光学和扫描电子显微镜(SEM)对 BisBAL NP-CPC ABM 进行了表征。使用盘扩散试验测量了抗菌潜力,并通过活/死试验和荧光显微镜确定了抗生物膜活性。在胰腺细胞系(Panc 03.27)上使用 MTT 试验和荧光显微镜进行活/死试验,分析其抗肿瘤活性:暴露 24 小时后(37°C,有氧条件下),5 µM BisBAL NP 可使肺炎双球菌的生长速度降低 77.9%,而 2.5 µM BisBAL NP 可使沙门氏菌、粪大肠杆菌和粪肠球菌的生长速度分别降低 82.9%、82.6% 和 78% (p)。BisBAL NPs-CPC ABM(比例为 10:1;分别为 500 µM 和 50 µM)可抑制所有分离细菌的生长,对肺炎双球菌、沙门氏菌、粪大肠杆菌和粪肠球菌分别产生 9.5、11.2、7 和 10.3 毫米的抑制光晕,而 100 µM 头孢曲松的抑制光晕分别为 6.5、9.5、8.5 和 9.8 毫米(p )。BisBAL NPs-CPC ABM 还能减少细菌生物膜,对肺炎双球菌、粪大肠杆菌、屎大肠杆菌和沙门氏菌的抑制率分别为 81.4%、74.5%、97.1% 和 79.5%。此外,BisBAL NPs-CPC ABM 可使 Panc 03.27 细胞的生长速度降低 76%,而不含药物的 ABM 只降低了 18%。GEM-ABM 可使肿瘤生长减少 73%。活/死试验证实,BisBAL NPs-CPC-ABM 和 GEM-ABM 对胰腺 03.27 细胞具有细胞毒性:结论:负载有 BisBAL NP 和 CPC 的藻酸盐膜具有抗菌和抗肿瘤双重功效。因此,它可用于癌症治疗和减少手术部位感染的发生。
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引用次数: 0
Retraction Influence of carbon and graphene oxide nanoparticle on the adhesive properties of dentin bonding polymer: A SEM, EDX, FTIR study. 回放 碳和氧化石墨烯纳米粒子对牙本质粘接聚合物粘接性能的影响:扫描电子显微镜(SEM)、电子衍射X射线(EDX)和傅立叶变换红外光谱(FTIR)研究。
IF 3.1 4区 医学 Q2 BIOPHYSICS Pub Date : 2024-01-01 DOI: 10.1177/22808000241273958
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引用次数: 0
In vitro chemical treatment of silk increases the expression of pro-inflammatory factors and facilitates degradation in rats. 对蚕丝进行体外化学处理会增加促炎因子的表达,并促进大鼠体内蚕丝的降解。
IF 2.5 4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-01-01 DOI: 10.1177/22808000231222704
Jinfeng Lv, Jieping Wang, Yao Zeng, Simeng Tian, Fei Wang, Yixue Zhai, Qian Zhou, Xiyue Luo, Xuanjie Zhang, Bin Liu, Chan Zhou

Objectives: Silk fiber is difficult to degrade in vivo, which limits its application in tissue engineering materials such as artificial nerves. Therefore, in this study aim to promote its degradation in vivo by chemical treating silk fibers in vitro.

Materials and methods: Sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), scanning electron microscopy (SEM) observations, mechanical test, Fourier transform infrared spectroscopy (FT-IR) measurements were used to investigate the degradation effect of chemicals (hydrochloric acid, phosphoric acid, acetic acid, sodium hydroxide, calcium hydroxide, sodium bicarbonate, and calcium chloride) on silk fiber in vitro. Immunofluorescence staining and transcriptome analysis were used to investigate the effect of inflammatory factors on the degradation of chemically treated silk fiber in rats.

Results: (1) Silks were separated into finer fibers in each group. (2) FT-IR absorption peaks of amides I, II, and III overlap in each group. (3) Silk degradation degree in each group was higher than that in an untreated group. The calcium chloride-treated group was completely degraded. (4) Fibronectin, collagen I, collagen III, integrin α and CD68 were immunofluorescence positive in all vegetation section. (5) There were no significant differences in the expressions of collagen I, collagen III, and fibronectin in the vegetations formed on the 14th day of subcutaneous implantation, while integrin α, CD68, TNF-α, IL-1b, and IL-23 express at higher levels with IL-10 at lower levels.

Conclusions: All chemicals could completely degrade silk; however, their degradation products were not the same. The chemicals change the mechanical properties of silk by separating it into finer fibers, which increase the contact surface area between the silk and tissue fluid, accelerating the degradation of monofilaments in vivo by promoting inflammation and macrophage activity through the increased and decreased expressions of pro- and anti-inflammatory factors, respectively.

目的:蚕丝纤维很难在体内降解,这限制了其在人工神经等组织工程材料中的应用。因此,本研究旨在通过在体外对蚕丝纤维进行化学处理,促进其在体内降解:采用硫酸盐-聚丙烯酰胺凝胶电泳(SDS-PAGE)、扫描电子显微镜(SEM)观察、力学测试、傅立叶变换红外光谱(FT-IR)测量等方法研究化学试剂(盐酸、磷酸、醋酸、氢氧化钠、氢氧化钙、碳酸氢钠和氯化钙)对体外蚕丝纤维的降解作用。免疫荧光染色和转录组分析用于研究炎症因子对大鼠化学处理丝纤维降解的影响。(2)各组酰胺 I、II 和 III 的傅立叶变换红外吸收峰重叠。(3)各组的蚕丝降解程度均高于未处理组。氯化钙处理组完全降解。(4)所有植被切片中的纤连蛋白、胶原 I、胶原 III、整合素 α 和 CD68 均呈免疫荧光阳性。(5)在皮下植入第 14 天形成的植被中,胶原 I、胶原 III 和纤连蛋白的表达无显著差异,而整合素 α、CD68、TNF-α、IL-1b 和 IL-23 的表达水平较高,IL-10 的表达水平较低:结论:所有化学物质都能完全降解蚕丝,但它们的降解产物却不尽相同。化学物质通过将蚕丝分离成更细的纤维改变了蚕丝的机械性能,从而增加了蚕丝与组织液的接触表面积,通过分别增加和减少促炎因子和抗炎因子的表达来促进炎症和巨噬细胞的活性,加速了单丝在体内的降解。
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引用次数: 0
Impact of combining dental composite brushes with modeling resins on the color stability and topographic features of composites. 牙科复合材料刷与造型树脂的结合对复合材料颜色稳定性和形貌特征的影响。
IF 3.1 4区 医学 Q2 BIOPHYSICS Pub Date : 2024-01-01 DOI: 10.1177/22808000241272487
Abdulrahman A Balhaddad, Faisal Alharamlah, Alhanoof Aldossary, Wejdan Almutairi, Turki Alshehri, Mary Anne S Melo, Afnan O Al-Zain, Eman H Ismail

This study explores the effect of using dental brushes with or without metacrylate-based modeling resins on long-term color stability and surface topographies of resin-based composites. This study examined the effects of two variables: (1) the type of brush used (Art brush, Micro-brush, or Mylar strip) and (2) the application of a modeling resin (applied or not applied). The specimens were artificially aged through 10,000 cycles of thermocycling and subsequently immersed in coffee for 30 days. Measurements of color and surface roughness were taken at baseline and after the aging, using a non-contact profilometer for surface roughness and a spectrophotometer for color. Data were analyzed using paired t-tests and one-way ANOVA. Resin-based composites smoothed with dental brushes or micro brushes without modeling resins exhibited lower color change (ΔE) than other groups. Paired t-tests revealed significant differences in average surface roughness (Ra) and valley depth (Rv) for each surfacing technique before and after aging (p ⩽ 0.01). The root means square average of the profile heights (Rq) significantly increased in the control and micro-brush groups (p ⩽ 0.01). In conclusion, the use of brushes in resin-based composites placement does not increase the susceptibility to staining. Instead, the inclusion of resin modeling contributes to discoloration over time.

本研究探讨了牙科用刷子涂抹或不涂抹偏丙烯酸酯类造型树脂对树脂基复合材料的长期颜色稳定性和表面形貌的影响。本研究考察了两个变量的影响:(1) 所用牙刷的类型(艺术牙刷、微型牙刷或聚酯条);(2) 所用造型树脂(涂抹或不涂抹)。试样通过 10,000 次热循环进行人工老化,然后在咖啡中浸泡 30 天。使用非接触式轮廓仪测量表面粗糙度,使用分光光度计测量颜色,分别在基线和老化后测量颜色和表面粗糙度。数据分析采用配对 t 检验和单因素方差分析。与其他组相比,用牙刷或不含造型树脂的微型刷磨平的树脂基复合材料的颜色变化(ΔE)较低。配对 t 检验显示,每种表面处理技术在老化前后的平均表面粗糙度 (Ra) 和谷深 (Rv) 都存在显著差异(p ⩽0.01)。对照组和微刷组的轮廓高度均方根平均值(Rq)明显增加(p ⩽0.01)。总之,在树脂基复合材料铺放过程中使用刷子不会增加染色的敏感性。相反,树脂模型的加入会随着时间的推移而导致褪色。
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引用次数: 0
Cnicus benedictus extract-loaded electrospun gelatin wound dressing for treating diabetic wounds: An in vitro and in vivo study. 用于治疗糖尿病伤口的含桔梗提取物的电纺明胶伤口敷料:体外和体内研究。
IF 2.5 4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2024-01-01 DOI: 10.1177/22808000241245298
Jing Zhang, Shen Shen, Shijie Zhu, Fan Jia, Jin Li, Yu Sun

In the current study, Cnicus benedictus extract was loaded into electrospun gelatin scaffolds for diabetic wound healing applications. Scaffolds were characterized in vitro by mechanical testing, cell culture assays, electron microscopy, cell migration assay, and antibacterial assay. In vivo wound healing study was performed in a rat model of diabetic wound. In vitro studies revealed fibrous architecture of our developed dressings and their anti-inflammatory properties. In addition, Cnicus benedictus extract-loaded wound dressings prevented bacterial penetration. In vivo study showed that wound size reduction, collagen deposition, and epithelial thickness were significantly greater in Cnicus benedictus extract-loaded scaffolds than other groups. Gene expression studies showed that the produced wound dressings significantly upregulated VEGF and IGF genes expression in diabetic wounds.

在目前的研究中,本草纲目提取物被载入电纺明胶支架,用于糖尿病伤口愈合。通过机械测试、细胞培养试验、电子显微镜、细胞迁移试验和抗菌试验对支架进行了体外表征。在大鼠糖尿病伤口模型中进行了体内伤口愈合研究。体外研究显示,我们开发的敷料具有纤维结构和抗炎特性。此外,敷料还能防止细菌渗透。体内研究表明,与其他组别相比,负载了蛇床子提取物的支架在伤口缩小、胶原沉积和上皮厚度方面都有显著提高。基因表达研究表明,所生产的伤口敷料能明显提高糖尿病伤口中血管内皮生长因子和 IGF 基因的表达。
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引用次数: 0
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Journal of Applied Biomaterials & Functional Materials
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