Molecular monitoring of treatment efficacy in human visceral leishmaniasis.

IF 1.5 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2024-05-01 DOI:10.1093/trstmh/trad103

Sutopa Roy, Srija Moulik, Surya Jyati Chaudhuri, Manab K Ghosh, R P Goswami, Bibhuti Saha, Mitali Chatterjee

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Abstract

Background: Focused efforts of the visceral leishmaniasis elimination program have led to a drastic decline in cases, and the present challenge is disease monitoring, which this study aimed to assess.

Methods: A Leishmania kinetoplastid-targeted qPCR quantified parasite load at disease presentation, and following treatment completion (n=49); an additional 80 cases were monitored after completion of treatment.

Results: The parasite load at disease presentation was 13 461.00 (2560.00-37764.00)/µg gDNA, which upon completion of treatment reduced in 47 of 49 cases to 1(1-1)/µg gDNA, p<0.0001. In 80 cases that presented >2 months post-treatment, their parasite burden similarly decreased to 1(1-1)/µg gDNA except in 6 of 80 cases, which were qPCR positive.

Conclusion: In 129 cases of visceral leishmaniasis, qPCR by quantification of parasite burden proved effective for monitoring treatment.

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人类内脏利什曼病疗效的分子监测。

背景：消除内脏利什曼病计划的集中努力已导致病例急剧下降，目前的挑战是疾病监测，本研究旨在对此进行评估：消除内脏利什曼病计划的集中努力已导致病例急剧下降，目前的挑战是疾病监测，本研究旨在对此进行评估：方法：利用利什曼原虫靶向 qPCR 对发病时和治疗结束后（49 例）的寄生虫量进行量化；治疗结束后对另外 80 例病例进行监测：结果：发病时的寄生虫量为 13 461.00 (2560.00-37764.00)/µg gDNA，完成治疗后，49 例中有 47 例的寄生虫量减少到 1(1-1)/µg gDNA，治疗后 2 个月，除 80 例中有 6 例 qPCR 阳性外，其他病例的寄生虫量同样减少到 1(1-1)/µg gDNA：结论：在 129 例内脏利什曼病病例中，通过量化寄生虫负荷进行 qPCR 检测可有效监测治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transactions of The Royal Society of Tropical Medicine and Hygiene 医学-公共卫生、环境卫生与职业卫生

CiteScore

4.00

自引率

9.10%

发文量

115

审稿时长

4-8 weeks

期刊介绍： Transactions of the Royal Society of Tropical Medicine and Hygiene publishes authoritative and impactful original, peer-reviewed articles and reviews on all aspects of tropical medicine.