Recent advances of single-cell RNA sequencing in toxicology research: Insight into hepatotoxicity and nephrotoxicity

Junhui Chen , Jiangpeng Wu , Yunmeng Bai , Chuanbin Yang , Jigang Wang
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Abstract

The advanced single-cell RNA sequencing (scRNA-seq) technology enables the measurement of gene expression levels and identification of cell-specific markers in individual cells, and the revealing of cell types, providing valuable insights into cell behavior. ScRNA-seq has emerged as an effective method for detecting rare cell populations, cellular heterogeneity, and cell-to-cell crosstalk under physiology and pathology conditions, thereby advancing the revolution of toxicology research. In this review, we first briefly introduce the concept of hepatotoxicity and nephrotoxicity, along with their underlying mechanisms. We then summarize the single-cell sequencing technology, with an emphasis on single-cell isolation techniques, particularly for the most used droplet-based methods. Finally, we review the recent advances in the application of single-cell RNA sequencing in studying hepatotoxicity and nephrotoxicity. Overall, this review provides a comprehensive understanding of the recent advances in hepatotoxicity and nephrotoxicity studies at a single-cell resolution.

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单细胞 RNA 测序在毒理学研究中的最新进展:深入了解肝毒性和肾毒性
先进的单细胞 RNA 测序(scRNA-seq)技术可测量单个细胞的基因表达水平,识别细胞特异性标记,并揭示细胞类型,为了解细胞行为提供有价值的信息。ScRNA-seq 已成为检测生理和病理条件下稀有细胞群、细胞异质性和细胞间串扰的有效方法,从而推动了毒理学研究的革命。在这篇综述中,我们首先简要介绍了肝毒性和肾毒性的概念及其内在机制。然后,我们总结了单细胞测序技术,重点是单细胞分离技术,尤其是最常用的基于液滴的方法。最后,我们回顾了单细胞 RNA 测序在肝毒性和肾毒性研究中应用的最新进展。总之,本综述全面介绍了单细胞分辨率肝毒性和肾毒性研究的最新进展。
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来源期刊
Current opinion in toxicology
Current opinion in toxicology Toxicology, Biochemistry
CiteScore
8.50
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审稿时长
64 days
期刊最新文献
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