The Effect of Different Sources of Unsaturated Fatty Acids on the Expression of IL-1β and TNFα Genes and Blood Factors in Sangesari Lambs Vaccinated against Foot and Mouth Disease

IF 0.6 4区 生物学 Q4 GENETICS & HEREDITY Russian Journal of Genetics Pub Date : 2024-01-15 DOI:10.1134/s1022795423140041
A. Ghorbani, A. A. Sadeghi, P. Shawrang, M. Chamani, F. Foroudi
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Abstract

We studied the effect of palmitic acid sources or n-3 and n-6 polyunsaturated fatty acids (PUFA) in the diet on the pro-inflammatory cytokine expression and the risk of ketosis in lambs received vaccination for foot and mouth disease (FMD) virus. We used 15 Sangsari male lambs with an aged 6 ± 1 month and an average weight of 42 ± 1 kg allocated randomly to three treatments: (1) PalOil group receiving a diet of calcium soap of palm oil fatty acid (FA) to receive palmitic acid (16:0), (2) SunOil group receiving a diet containing calcium soap of sunflower oil FAs to receive linoleic acid (n-6 18:2) and (3) LinOil group receiving a diet containing calcium soap of linseed oil FA to receive α-linolenic acid (n-3 18:3). The lambs received the iso-nitrogenous and iso-caloric diet for 28 days: an adaptation period of 21 days and a sampling period of 7 days. The lambs were separately housed and could drink water. The interleukin-1β (IL-1β) mRNA expression was lower in the LinOil groups than in the PalOil and SunOil groups (P < 0.05). Also, the tumor necrosis factor-α (TNFα) mRNA expression showed an increase in the PalOil group than in the SunOil and LinOil groups, and the lowest expression of TNFα mRNA was found in the LinOil group (P < 0.05). Higher glucose blood level (P < 0.05) was measured in the PalOil group than in lambs on SunOil and LinOil diets. No significant differences were observed between treatments in BHBA and cortisol blood levels (P > 0.05). Feeding an α-linolenic acid diet to lambs after vaccination against FMD improved glucose uptake, decreased the risk of ketosis, and inhibited the expression of pro-inflammatory cytokines (TNFα and IL-1).

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不同来源的不饱和脂肪酸对接种口蹄疫疫苗的桑格萨里羔羊 IL-1β 和 TNFα 基因及血液因子表达的影响
摘要 我们研究了日粮中棕榈酸来源或 n-3 和 n-6 多不饱和脂肪酸 (PUFA) 对接种口蹄疫 (FMD) 病毒疫苗的羔羊促炎细胞因子表达和酮病风险的影响。我们使用了 15 只桑萨里雄性羔羊,它们的年龄为 6 ± 1 个月,平均体重为 42 ± 1 千克,随机分配到三种处理中:(1) PalOil 组接受棕榈油脂肪酸(FA)钙皂日粮,以获得棕榈酸(16:0);(2) SunOil 组接受葵花籽油脂肪酸钙皂日粮,以获得亚油酸(n-6 18:2);(3) LinOil 组接受亚麻籽油脂肪酸钙皂日粮,以获得α-亚麻酸(n-3 18:3)。羔羊接受等氮等热量日粮 28 天:适应期 21 天,采样期 7 天。羔羊分开饲养,可以饮水。LinOil 组的白细胞介素-1β(IL-1β)mRNA 表达量低于 PalOil 组和 SunOil 组(P < 0.05)。此外,PalOil 组的肿瘤坏死因子-α(TNFα)mRNA 表达量比 SunOil 组和 LinOil 组有所增加,而 LinOil 组的 TNFα mRNA 表达量最低(P < 0.05)。与食用 SunOil 和 LinOil 日粮的羔羊相比,食用 PalOil 日粮的羔羊血糖水平更高(P < 0.05)。不同处理之间的 BHBA 和皮质醇血液水平无明显差异(P > 0.05)。给接种 FMD 疫苗后的羔羊饲喂 α-亚麻酸日粮可改善葡萄糖吸收,降低酮症风险,并抑制促炎细胞因子(TNFα 和 IL-1)的表达。
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来源期刊
Russian Journal of Genetics
Russian Journal of Genetics 生物-遗传学
CiteScore
1.00
自引率
33.30%
发文量
126
审稿时长
1 months
期刊介绍: Russian Journal of Genetics is a journal intended to make significant contribution to the development of genetics. The journal publishes reviews and experimental papers in the areas of theoretical and applied genetics. It presents fundamental research on genetic processes at molecular, cell, organism, and population levels, including problems of the conservation and rational management of genetic resources and the functional genomics, evolutionary genomics and medical genetics.
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