Electrochemical detection of glutathione based on accelerated CRISPR/Cas12a trans-cleavage with MnO2 nanosheets†

IF 4.3 2区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Chemical Communications Pub Date : 2024-02-15 DOI:10.1039/d3cc06186h
Renpeng Xia , Nan Ouyang , Tingting Wang , Yuan Zhuang , Peng Miao
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Abstract

The CRISPR/Cas12a system is accelerated by glutathione-mediated reduction of MnO2 nanosheets. By monitoring the trans-cleavage of the DNA probe, an electrochemical method for glutathione assay is fabricated, with the detection limit of 3.5 pM. It provides a promising tool for plasma analysis with satisfactory performance, indicating the broad application prospects of this glutathione assay.

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基于加速 CRISPR/Cas12a 与 MnO2 纳米片反式裂解的谷胱甘肽电化学检测技术
谷胱甘肽介导的 MnO2 纳米片还原加速了 CRISPR/Cas12a 系统。通过监测 DNA 探针的反式裂解事件,建立了谷胱甘肽检测的电化学方法,其检测限为 3.5 pM。它为血浆分析提供了一种性能令人满意的工具,表明谷胱甘肽检测法具有广阔的应用前景。
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来源期刊
Chemical Communications
Chemical Communications 化学-化学综合
CiteScore
8.60
自引率
4.10%
发文量
2705
审稿时长
1.4 months
期刊介绍: ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.
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