The mechanisms of tumor necrosis factor α in regulating Krüpple-like factor 4 expression in SK-BR-3 breast cancer cells.

IF 1.4 4区医学 Q4 ONCOLOGY Asia-Pacific journal of clinical oncology Pub Date : 2024-01-18 DOI:10.1111/ajco.14046

Caiyun Liu, Chengbao Feng, Haifei Li, Erying Zhang, Bo Liu, Ying Wang, Peng Wang

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引用次数: 0

Abstract

Objective: To explore the expression and functional role of Krüpple-like factor 4 (KLF4) protein stimulated by tumor necrosis factor α (TNF-α) in SK-BR-3 breast cancer cells.

Methods: SK-BR-3 cells were stimulated with various concentrations of TNF-α at 0, 1, 5, 10, and 20 ng/mL. Expression levels of KLF4 protein were detected by Western blotting. In the detection of apoptosis, flow cytometry, and DAPI staining were used for detecting the level of apoptosis.

Results: KLF4 expression was markedly elevated following stimulation of SK-BR-3 with TNF-α. At the same time, the expression of KLF4 protein increased gradually with the increase of TNF-α stimulation concentration. TNF-α stimulation of SK-BR-3 cells increased apoptosis as measured by apoptosis levels. By overexpressing KLF4 protein in SK-BR-3 cells, it similarly increased apoptosis and promoted cell death of SK-BR-3 cells.

Conclusion: TNF-α promotes KLF4 expression, while TNF-α promotes apoptosis in SK-BR-3 cells, a process that may be due to elevated KLF4 protein expression.

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肿瘤坏死因子α调节SK-BR-3乳腺癌细胞中Krüpple样因子4表达的机制

目的探讨肿瘤坏死因子α（TNF-α）刺激的Krüpple样因子4（KLF4）蛋白在SK-BR-3乳腺癌细胞中的表达和功能作用：用不同浓度的 TNF-α（0、1、5、10 和 20 ng/mL）刺激 SK-BR-3 细胞。用 Western 印迹法检测 KLF4 蛋白的表达水平。在检测细胞凋亡时，使用流式细胞仪和 DAPI 染色检测细胞凋亡水平：结果：TNF-α刺激SK-BR-3后，KLF4的表达明显升高。同时，随着TNF-α刺激浓度的增加，KLF4蛋白的表达量也逐渐增加。以细胞凋亡水平衡量，TNF-α刺激SK-BR-3细胞会增加细胞凋亡。通过在SK-BR-3细胞中过表达KLF4蛋白，同样增加了细胞凋亡，促进了SK-BR-3细胞的死亡：结论：TNF-α促进KLF4的表达，而TNF-α促进SK-BR-3细胞的凋亡，这一过程可能是由于KLF4蛋白表达的升高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asia-Pacific journal of clinical oncology 医学-肿瘤学

CiteScore

3.40

自引率

0.00%

发文量

175

审稿时长

6-12 weeks

期刊介绍： Asia–Pacific Journal of Clinical Oncology is a multidisciplinary journal of oncology that aims to be a forum for facilitating collaboration and exchanging information on what is happening in different countries of the Asia–Pacific region in relation to cancer treatment and care. The Journal is ideally positioned to receive publications that deal with diversity in cancer behavior, management and outcome related to ethnic, cultural, economic and other differences between populations. In addition to original articles, the Journal publishes reviews, editorials, letters to the Editor and short communications. Case reports are generally not considered for publication, only exceptional papers in which Editors find extraordinary oncological value may be considered for review. The Journal encourages clinical studies, particularly prospectively designed clinical trials.