The trade-off between individual metabolic specialization and versatility determines the metabolic efficiency of microbial communities.

Miaoxiao Wang, Xiaoli Chen, Yuan Fang, Xin Zheng, Ting Huang, Yong Nie, Xiao-Lei Wu
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Abstract

In microbial systems, a metabolic pathway can be either completed by one autonomous population or distributed among a consortium performing metabolic division of labor (MDOL). MDOL facilitates the system's function by reducing the metabolic burden; however, it may hinder the function by reducing the exchange efficiency of metabolic intermediates among individuals. As a result, the function of a community is influenced by the trade-offs between the metabolic specialization and versatility of individuals. To experimentally test this hypothesis, we deconstructed the naphthalene degradation pathway into four steps and introduced them individually or combinatorically into different strains with varying levels of metabolic specialization. Using these strains, we engineered 1,456 synthetic consortia and found that 74 consortia exhibited higher degradation function than both the autonomous population and rigorous MDOL consortium. Quantitative modeling provides general strategies for identifying the most effective MDOL configuration. Our study provides critical insights into the engineering of high-performance microbial systems.

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个体代谢专业化与多功能性之间的权衡决定了微生物群落的代谢效率。
在微生物系统中,一条代谢途径既可以由一个独立的种群完成,也可以分布在进行代谢分工(MDOL)的群体中。新陈代谢分工(MDOL)可以减轻代谢负担,从而促进系统功能的发挥;但也可能会降低代谢中间产物在个体间的交换效率,从而阻碍系统功能的发挥。因此,群落的功能受到个体代谢专业性和多功能性之间权衡的影响。为了通过实验验证这一假设,我们将萘降解途径分解为四个步骤,并将它们单独或组合引入具有不同代谢特化水平的不同菌株中。利用这些菌株,我们设计了 1,456 个合成联合体,发现 74 个联合体的降解功能高于自主群体和严格的 MDOL 联合体。定量建模为确定最有效的 MDOL 配置提供了一般策略。我们的研究为高性能微生物系统工程提供了重要见解。
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