Yinan Chen, Xiaolong Qiao, Changqing Liu, Jin Zhang, Tao Sun, Ling Kong, Xinru Zhang, Wencheng Song, Chuandong Cheng, Guohua Ni
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引用次数: 0
Abstract
In this work, the effects of cold atmospheric-pressure plasma (CAP) on glioblastoma are evaluated comprehensively. After CAP treatment, U251 cell viability, migration, and invasion functions were inhibited, while an appropriate dose of CAP had no inhibitory effect on human brain glial cell line cells. Western blots indicated that expression of caspase-3 was upregulated with ki-67 expression downregulated. Moreover, mitochondrial membrane potential decreased, and energy metabolisms of U251 cells were influenced afterward. TUNEL assays and comet assays suggested the DNA damage of U251 cells after CAP treatment. Furthermore, as one of the DNA damage responses associated pathways, the AKT (AKT8 virus oncogene cellular homolog) signaling pathway was also indicated in the work. The findings raise great promise for clinical applications of CAP in glioblastoma treatments.
本研究全面评估了冷大气压等离子体(CAP)对胶质母细胞瘤的影响。经 CAP 处理后,U251 细胞的活力、迁移和侵袭功能均受到抑制,而适当剂量的 CAP 对人脑神经胶质细胞系细胞无抑制作用。Western 印迹显示,Caspase-3 的表达上调,ki-67 的表达下调。此外,线粒体膜电位降低,U251 细胞的能量代谢受到影响。TUNEL 试验和彗星试验表明,CAP 处理后 U251 细胞的 DNA 受到损伤。此外,作为DNA损伤反应的相关途径之一,AKT(AKT8病毒癌基因细胞同源物)信号通路也在研究中得到了证实。这些发现为 CAP 在胶质母细胞瘤治疗中的临床应用带来了巨大希望。
期刊介绍:
Plasma Processes & Polymers focuses on the interdisciplinary field of low temperature plasma science, covering both experimental and theoretical aspects of fundamental and applied research in materials science, physics, chemistry and engineering in the area of plasma sources and plasma-based treatments.