Study on the mechanism of Panax notoginseng–Salvia miltiorrhiza herb pair on invigorating blood circulation and eliminating blood stasis by blocking the conversion of arachidonic acid to prostaglandin

IF 2.5 4区医学 Q3 CHEMISTRY, MEDICINAL Journal of Natural Medicines Pub Date : 2024-01-23 DOI:10.1007/s11418-023-01773-z

Rui Zeng, Yuefan Zhang, Shengtong Shi, Xianqin Long, Haixia Zhang, Min Wang, Jianfeng Shi, Ye Jiang, Bin Chen

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Abstract

We combined untargeted and targeted metabolomics to explore the mechanism of blood circulation and blood stasis activation in the traditional Chinese herb pair Panax notoginseng–Salvia miltiorrhiza (PS). In this study, the right hind limb of SD rats was struck by a 1 kg weight, causing traumatic blood stasis (TBS) model, then the rats were gavaged with PS (at ratios of 1:0, 0:1, 3:1, 1:1, and 1:3) for 5 consecutive days. At the end of treatment, blood samples were collected for blood rheology and metabolomics analysis, and muscle tissues of injured limbs were used for HE staining and q-PCR analysis. The results showed that different ratios of PS reduced swelling and improved stasis and blood viscosity in the injured limbs of rats, and intervened in metabolism by modulating 11, 11, 17, 15, and 13 differential metabolites, respectively. The PS (3:1) shows the best treatment effect and the most differential metabolites regression. Targeted metabolomics shows that PS (3:1) can increase the content of AA, and reduce the content of PGF₂-α by down-regulating the expression of enzymes Ptgs1 and Cbrl12 and up-regulating the expression of enzyme Hpgd. These results suggested that the PS herb pair exerts its blood stasis activating effects by blocking the conversion of arachidonic acid to prostaglandins.

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研究三七-丹参药对通过阻断花生四烯酸向前列腺素的转化来活血化瘀的机理。

我们将非靶向和靶向代谢组学相结合，探讨了中药三七-丹参（PS）的活血化瘀机制。本研究先用1公斤重物击打SD大鼠的右后肢，造成创伤性血瘀（TBS）模型，然后连续5天灌胃PS（比例分别为1:0、0:1、3:1、1:1和1:3）。治疗结束后，采集血液样本进行血液流变学和代谢组学分析，并对受伤肢体的肌肉组织进行 HE 染色和 q-PCR 分析。结果表明，不同比例的 PS 能减轻大鼠受伤肢体的肿胀，改善瘀血和血液粘度，并通过调节 11、11、17、15 和 13 种不同的代谢物来干预代谢。PS（3:1）的治疗效果最好，调节的差异代谢物也最多。靶向代谢组学研究表明，PS（3:1）通过下调 Ptgs1 和 Cbrl12 酶的表达，上调 Hpgd 酶的表达，可以增加 AA 的含量，降低 PGF2-α的含量。这些结果表明，PS 草药对通过阻断花生四烯酸向前列腺素的转化来发挥其活血化瘀的作用。

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来源期刊

Journal of Natural Medicines 医学-药学

CiteScore

6.90

自引率

3.00%

发文量

审稿时长

1.7 months

期刊介绍： The Journal of Natural Medicines is an international journal publishing original research in naturally occurring medicines and their related foods and cosmetics. It covers: -chemistry of natural products -biochemistry of medicinal plants -pharmacology of natural products and herbs, including Kampo formulas and traditional herbs -botanical anatomy -cultivation of medicinal plants. The journal accepts Original Papers, Notes, Rapid Communications and Natural Resource Letters. Reviews and Mini-Reviews are generally invited.