Promoter engineering of natural product biosynthetic gene clusters in actinomycetes: concepts and applications

IF 10.2 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Natural Product Reports Pub Date : 2024-04-24 DOI:10.1039/d3np00049d

Chang-Hun Ji , Hyun-Woo Je , Hiyoung Kim , Hahk-Soo Kang

引用次数: 0

Abstract

Covering 2011 to 2022

Low titers of natural products in laboratory culture or fermentation conditions have been one of the challenging issues in natural products research. Many natural product biosynthetic gene clusters (BGCs) are also transcriptionally silent in laboratory culture conditions, making it challenging to characterize the structures and activities of their metabolites. Promoter engineering offers a potential solution to this problem by providing tools for transcriptional activation or optimization of biosynthetic genes. In this review, we summarize the 10 years of progress in promoter engineering approaches in natural products research focusing on the most metabolically talented group of bacteria actinomycetes.

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放线菌天然产物生物合成基因簇的启动子工程：概念与应用。

覆盖 2011 年至 2022 年天然产物在实验室培养或发酵条件下的低滴度一直是天然产物研究中的挑战性问题之一。许多天然产物生物合成基因簇（BGCs）在实验室培养条件下也处于转录沉默状态，这给鉴定其代谢产物的结构和活性带来了挑战。启动子工程为生物合成基因的转录激活或优化提供了工具，从而为这一问题提供了潜在的解决方案。在这篇综述中，我们总结了启动子工程方法在天然产品研究中的 10 年进展，重点是代谢天赋最高的放线菌属。

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来源期刊

Natural Product Reports 化学-生化与分子生物学

CiteScore

21.20

自引率

3.40%

发文量

127

审稿时长

1.7 months

期刊介绍： Natural Product Reports (NPR) serves as a pivotal critical review journal propelling advancements in all facets of natural products research, encompassing isolation, structural and stereochemical determination, biosynthesis, biological activity, and synthesis. With a broad scope, NPR extends its influence into the wider bioinorganic, bioorganic, and chemical biology communities. Covering areas such as enzymology, nucleic acids, genetics, chemical ecology, carbohydrates, primary and secondary metabolism, and analytical techniques, the journal provides insightful articles focusing on key developments shaping the field, rather than offering exhaustive overviews of all results. NPR encourages authors to infuse their perspectives on developments, trends, and future directions, fostering a dynamic exchange of ideas within the natural products research community.