The application of High-Resolution Nuclear Magnetic Resonance (HR NMR) in metabolomic analyses of meconium and stool in newborns. A preliminary pilot study of MABEL project: Metabolomics approach for the assessment of Baby-Mother Enteric Microbiota Legacy

IF 2.4 Q3 NUTRITION & DIETETICS PharmaNutrition Pub Date : 2024-01-19 DOI:10.1016/j.phanu.2024.100378
Alessia Trimigno , Beata Łoniewska , Karolina Skonieczna-Żydecka , Mariusz Kaczmarczyk , Igor Łoniewski , Gianfranco Picone
{"title":"The application of High-Resolution Nuclear Magnetic Resonance (HR NMR) in metabolomic analyses of meconium and stool in newborns. A preliminary pilot study of MABEL project: Metabolomics approach for the assessment of Baby-Mother Enteric Microbiota Legacy","authors":"Alessia Trimigno ,&nbsp;Beata Łoniewska ,&nbsp;Karolina Skonieczna-Żydecka ,&nbsp;Mariusz Kaczmarczyk ,&nbsp;Igor Łoniewski ,&nbsp;Gianfranco Picone","doi":"10.1016/j.phanu.2024.100378","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The microbial population living in our intestines plays a key role in several metabolic, nutritional, physiological, and immunological processes. It is known that infant gut microbiota composition has both horizontal transmission delivery and environmental conditions and a vertical one, from mother to child, related to how the infant is fed (breastfed or infant formula). Detailed information on the composition of meconium and feces from newborns may help predict the most prevalent and hazardous conditions affecting pregnancies, mothers, and babies, including pre-term birth, preeclampsia, and gestational diabetes mellitus for example.</p></div><div><h3>Methods</h3><p>This work aims to demonstrate the feasibility of the whole High-Resolution proton Nuclear Magnetic Resonance (1H HR NMR) procedure in metabolomic analysis in preterm newborns. Thus, multiple samples of meconium and stool were collected from 3 pairs of premature twins and their metabolite profiles were acquired and exploited by combining the NMR technique with univariate and multivariate analysis.</p></div><div><h3>Results</h3><p>The analysis showed that an impact on the metabolite profile was visible concerning both the sex of the newborns and the couplet of origin. Most of the variation between twin couplets was seen with butyric acid concentration in meconium/stool samples.</p></div><div><h3>Conclusions</h3><p>Despite the low number of samples, the described NMR procedure showed to be a suitable approach to evaluate the similarities of the molecular profiles of different samples, offering a non-invasive and informative approach to understanding the metabolic and nutritional status of preterm infants. Future metabolomic analysis should be supported by microbiome analysis, such multiomic approach will provide more complex view on development of preterm newborns.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":null,"pages":null},"PeriodicalIF":2.4000,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213434424000045/pdfft?md5=2e787a7fd6eb933f3dbf7bbe44089a37&pid=1-s2.0-S2213434424000045-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PharmaNutrition","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213434424000045","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
引用次数: 0

Abstract

Background

The microbial population living in our intestines plays a key role in several metabolic, nutritional, physiological, and immunological processes. It is known that infant gut microbiota composition has both horizontal transmission delivery and environmental conditions and a vertical one, from mother to child, related to how the infant is fed (breastfed or infant formula). Detailed information on the composition of meconium and feces from newborns may help predict the most prevalent and hazardous conditions affecting pregnancies, mothers, and babies, including pre-term birth, preeclampsia, and gestational diabetes mellitus for example.

Methods

This work aims to demonstrate the feasibility of the whole High-Resolution proton Nuclear Magnetic Resonance (1H HR NMR) procedure in metabolomic analysis in preterm newborns. Thus, multiple samples of meconium and stool were collected from 3 pairs of premature twins and their metabolite profiles were acquired and exploited by combining the NMR technique with univariate and multivariate analysis.

Results

The analysis showed that an impact on the metabolite profile was visible concerning both the sex of the newborns and the couplet of origin. Most of the variation between twin couplets was seen with butyric acid concentration in meconium/stool samples.

Conclusions

Despite the low number of samples, the described NMR procedure showed to be a suitable approach to evaluate the similarities of the molecular profiles of different samples, offering a non-invasive and informative approach to understanding the metabolic and nutritional status of preterm infants. Future metabolomic analysis should be supported by microbiome analysis, such multiomic approach will provide more complex view on development of preterm newborns.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
高分辨率核磁共振(HR-NMR)在新生儿胎粪和粪便代谢组学分析中的应用。MABEL 项目的初步试点研究:代谢组学方法评估母婴肠道微生物群遗产
背景生活在我们肠道中的微生物群在多个代谢、营养、生理和免疫过程中发挥着关键作用。众所周知,婴儿肠道微生物群的组成既与分娩和环境条件有关,也与母亲对婴儿的喂养方式(母乳喂养或婴儿配方奶粉)有关。有关新生儿粪便和胎粪组成的详细信息可能有助于预测影响妊娠、母亲和婴儿的最普遍和最危险的情况,例如早产、子痫前期和妊娠糖尿病。因此,我们从 3 对早产双胞胎身上采集了多份胎粪和粪便样本,通过核磁共振技术与单变量和多变量分析相结合,获得并利用了它们的代谢物谱。结论尽管样本数量较少,但所描述的核磁共振程序是评估不同样本分子特征相似性的合适方法,为了解早产儿的代谢和营养状况提供了一种非侵入性和信息丰富的方法。未来的代谢组分析应辅以微生物组分析,这种多组学方法将为早产新生儿的发育提供更复杂的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
PharmaNutrition
PharmaNutrition Agricultural and Biological Sciences-Food Science
CiteScore
5.70
自引率
3.10%
发文量
33
审稿时长
12 days
期刊最新文献
Advancements in psoriasis management: Integrating nutrient supplement with gut-brain-skin connection Can L-Methionine and S-Adenosyl-L-Methionine Effectively Mitigate Scopolamine-Induced Cognitive and Motor Deficits in Mice? Melatonin supplementation in preclinical colitis models: A systematic review and dose-response meta-analysis on inflammation, oxidative stress, and colon repair Efficacy and safety of liraglutide on C-reactive protein (CRP) in adults with type 2 diabetes: A GRADE-assessed systematic review and dose-response meta-analysis of controlled trials Editorial Board
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1