Application of sandpaper spray ionization mass spectrometry to comprehensively examine maple leaves infected with distinct fungi

IF 1.9 3区 化学 Q3 BIOCHEMICAL RESEARCH METHODS Journal of Mass Spectrometry Pub Date : 2024-01-23 DOI:10.1002/jms.5000
Leonardo Parasecolo, Laurentiu G. Dabija, Rani Shouk, Dani Shouk, Rodinei Augusti, Demian R. Ifa
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Abstract

This study describes a novel application for sandpaper spray ionization mass spectrometry (SPS-MS), to examine the surface of maple tree (Acer sp.) leaves. By comparing mass spectrometry fingerprints, healthy leaves from those infected with powdery mildew and Rhytisma acerinum were distinguished. Leaves were grated with sandpaper, cut into triangles, and placed before the mass spectrometer, with the addition of a methanol-formic acid solution. Multivariate statistical analysis categorized the samples into three groups. Overall, SPS-MS effectively analyzed leaves with infectious microorganisms, potentially aiding in the creation of fungal identification databanks.

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应用砂纸喷雾电离质谱法全面检测感染不同真菌的枫叶。
本研究介绍了砂纸喷雾电离质谱法(SPS-MS)在检测枫树(Acer sp.)叶片表面方面的新应用。通过比较质谱指纹图谱,可将健康叶片与感染白粉病和 Rhytisma acerinum 的叶片区分开来。叶片用砂纸磨碎,切成三角形,放在质谱仪前,加入甲醇-甲酸溶液。多元统计分析将样本分为三组。总之,SPS-MS 能有效分析带有传染性微生物的树叶,可能有助于建立真菌鉴定数据库。
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来源期刊
Journal of Mass Spectrometry
Journal of Mass Spectrometry 化学-光谱学
CiteScore
5.10
自引率
0.00%
发文量
84
审稿时长
1.5 months
期刊介绍: The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions. The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.
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