{"title":"Moringa oleifera-mediated iron oxide nanoparticles, characterization and their anti-proliferative potential on MDA-MB 231 human breast cancer cells.","authors":"Huzaifa Umar, Maryam Rabiu Aliyu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Iron oxide nanoparticles (Fe<sub>2</sub>O<sub>3</sub> NPs) stabilized with Moringa oleifera (M.O.) were successfully synthesized. The study aimed to explore the cytotoxic, anti-proliferative and anti-microbial potential of Fe<sub>2</sub>O<sub>3</sub> NPs through various assays, including trypan blue, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), wound healing and disc diffusion assay. The Moringa oleifera-mediated Fe<sub>2</sub>O<sub>3</sub> NPs (M O Fe<sub>2</sub>O<sub>3</sub> NPs) underwent analysis using various techniques, including Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), UV-vis spectroscopy (UV-vis), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). FTIR, XRD and SEM-EDX results confirmed the successful synthesis of Fe<sub>2</sub>O<sub>3</sub> NPs. The UV-Vis spectra analysis indicated an absorption peak at 314 nm, ensuring both the successful synthesis and remarkable stability of the nanoparticles. The nanoparticles displayed a uniform spherical morphology and contained Fe, O, confirming the formation of M O Fe<sub>2</sub>O<sub>3</sub> NPs. Cytotoxic and anti-proliferative potential on MDA-MB 231 human breast cancer cells were observed with various concentrations of M O Fe<sub>2</sub>O<sub>3</sub> NPs and the cytotoxicity result revealed an IC<sub>50</sub> of 69.7μg/mL. In conclusion, stable Fe<sub>2</sub>O<sub>3</sub> NPs were synthesized using a methanolic extract of M.O. and they demonstrated antimicrobial activity against both gram-negative and gram-positive bacteria. The nanoparticles exhibited cytotoxicity and anti-proliferative activity on MDA-MB 231 human breast cancer cell lines.</p>","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pakistan journal of pharmaceutical sciences","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
Abstract
Iron oxide nanoparticles (Fe2O3 NPs) stabilized with Moringa oleifera (M.O.) were successfully synthesized. The study aimed to explore the cytotoxic, anti-proliferative and anti-microbial potential of Fe2O3 NPs through various assays, including trypan blue, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), wound healing and disc diffusion assay. The Moringa oleifera-mediated Fe2O3 NPs (M O Fe2O3 NPs) underwent analysis using various techniques, including Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), UV-vis spectroscopy (UV-vis), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). FTIR, XRD and SEM-EDX results confirmed the successful synthesis of Fe2O3 NPs. The UV-Vis spectra analysis indicated an absorption peak at 314 nm, ensuring both the successful synthesis and remarkable stability of the nanoparticles. The nanoparticles displayed a uniform spherical morphology and contained Fe, O, confirming the formation of M O Fe2O3 NPs. Cytotoxic and anti-proliferative potential on MDA-MB 231 human breast cancer cells were observed with various concentrations of M O Fe2O3 NPs and the cytotoxicity result revealed an IC50 of 69.7μg/mL. In conclusion, stable Fe2O3 NPs were synthesized using a methanolic extract of M.O. and they demonstrated antimicrobial activity against both gram-negative and gram-positive bacteria. The nanoparticles exhibited cytotoxicity and anti-proliferative activity on MDA-MB 231 human breast cancer cell lines.
期刊介绍:
Pakistan Journal of Pharmaceutical Sciences (PJPS) is a peer reviewed multi-disciplinary pharmaceutical sciences journal. The PJPS had its origin in 1988 from the Faculty of Pharmacy, University of Karachi as a biannual journal, frequency converted as quarterly in 2005, and now PJPS is being published as bi-monthly from January 2013.
PJPS covers Biological, Pharmaceutical and Medicinal Research (Drug Delivery, Pharmacy Management, Molecular Biology, Biochemical, Pharmacology, Pharmacokinetics, Phytochemical, Bio-analytical, Therapeutics, Biotechnology and research on nano particles.