Circulating microRNA profiling identifies microRNAs linked to prediabetes associated with alcohol dependence syndrome.

Palaniswamy Ramaswamy, Athira S V, Pratibha Misra, V S Chauhan, Arka Adhvaryu, Anurodh Gupta, Ankita G, Sibin M K
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Abstract

Background: MicroRNAs are abundant in serum and have emerged as important regulators of gene expression, implicating them in a wide range of diseases. The purpose of this study was to discover and validate serum miRNAs in prediabetes associated with alcohol dependence syndrome (ADS).

Method: Serum samples from ADS patients with or without prediabetes and normoglycemic controls were subjected to microarray. Validation of identified candidate miRNAs was performed by RT-qPCR. Additionally, GO and KEGG pathway analyses were carried out to uncover target genes anticipated to be controlled by the candidate miRNAs.

Results: Notably, 198, and 172 miRNAs were differentially expressed in ADS-patients with or without prediabetes compared to healthy controls, and 7 miRNAs in ADS-patients with prediabetes compared to ADS-normoglycemic patients, respectively. Furthermore, hsa-miR-320b and hsa-miR-3135b were differentially expressed exclusively in ADS-patients with prediabetes, and this was further validated. Interestingly, GO and KEGG pathway analysis revealed that genes predicted to be modulated by the candidates were considerably enriched in numerous diabetes-related biological processes and pathways.

Conclusion: Our findings revealed that ADS-patients with or without prediabetes have different sets of miRNAs compared to normoglycemic healthy subjects. We propose serum hsa-miR-320b and hsa-miR-3135b as potential biomarkers for the diagnosis of prediabetes in ADS-patients.

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循环微RNA分析确定了与酒精依赖综合征相关的糖尿病前期有关的微RNA。
背景:微RNA在血清中含量丰富,已成为基因表达的重要调控因子,与多种疾病有关。本研究旨在发现和验证与酒精依赖综合征(ADS)相关的糖尿病前期患者的血清 miRNA:方法:对伴有或不伴有糖尿病前期的 ADS 患者和血糖正常对照组的血清样本进行芯片分析。通过 RT-qPCR 验证已确定的候选 miRNA。此外,还进行了 GO 和 KEGG 通路分析,以发现候选 miRNAs 预期控制的靶基因:值得注意的是,与健康对照组相比,有糖尿病前期或无糖尿病前期的 ADS 患者分别有 198 和 172 个 miRNAs 有差异表达;与 ADS 正常血糖患者相比,有糖尿病前期的 ADS 患者分别有 7 个 miRNAs 有差异表达。此外,hsa-miR-320b和hsa-miR-3135b只在糖尿病前期ADS患者中有差异表达,这一点得到了进一步验证。有趣的是,GO 和 KEGG 通路分析表明,候选基因预测被调节的基因在许多与糖尿病相关的生物过程和通路中都有相当大的富集:我们的研究结果表明,与血糖正常的健康受试者相比,患有或不患有糖尿病前期的 ADS 患者具有不同的 miRNAs 组。我们建议将血清中的 hsa-miR-320b 和 hsa-miR-3135b 作为诊断 ADS 患者糖尿病前期的潜在生物标记物。
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