Molecular detection and MST genotyping of Coxiella burnetii in ruminants and stray dogs and cats in Northern Algeria

IF 2 3区 农林科学 Q4 IMMUNOLOGY Comparative Immunology Microbiology and Infectious Diseases Pub Date : 2024-01-24 DOI:10.1016/j.cimid.2024.102126
H. Ghaoui , I. Bitam , S. Zaidi , N. Achour , S. Zenia , T. Idres , P.E. Fournier
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Abstract

Aiming at identifying the reservoir and contamination sources of Coxiella burnetii in Northern Algeria, we investigated the molecular presence of the bacterium in 599 samples (blood, placenta, liver, spleen, and uterus) collected from cattle, sheep, dogs and cats. Our qPCR results showed that 15/344 (4.36%) blood samples and six/255 (2.35%) organ specimens were positive for C. burnetii. In cattle, three (4%) blood and liver samples were positive. In sheep, one blood (1.19%) and 3 (8.57%) placenta samples were positive. At the Algiers dog pound, 8 (10%) and 3 (5%) blood samples were qPCR positivein dogs and cats, respectively. In addition, MST genotyping showed that MST 33 was present in cattle and sheep, MST 20 in cattle,andMST 21 in dogs and cats.

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阿尔及利亚北部反刍动物和流浪猫狗中烧伤柯西氏杆菌的分子检测和 MST 基因分型
为了确定阿尔及利亚北部烧伤柯西氏菌的贮藏地和污染源,我们调查了从牛、羊、狗和猫身上采集的 599 份样本(血液、胎盘、肝脏、脾脏和子宫)中该细菌的分子存在情况。我们的 qPCR 结果显示,15/344(4.36%)份血液样本和 6/255(2.35%)份器官样本对烧伤桿菌呈阳性。在牛中,3 份(4%)血液和肝脏样本呈阳性。在绵羊中,1 份血液样本(1.19%)和 3 份胎盘样本(8.57%)呈阳性。在阿尔及尔狗场,狗和猫的血液样本中分别有 8 份(10%)和 3 份(5%)呈 qPCR 阳性。此外,MST 基因分型显示,牛和羊体内存在 MST 33,牛体内存在 MST 20,狗和猫体内存在 MST 21。
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来源期刊
CiteScore
4.60
自引率
0.00%
发文量
102
审稿时长
40 days
期刊介绍: Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine . The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.
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