Validation of a molecular sex marker in three sturgeons from eastern North America

IF 0.9 4区环境科学与生态学 Q4 BIODIVERSITY CONSERVATION Conservation Genetics Resources Pub Date : 2024-01-27 DOI:10.1007/s12686-024-01346-6

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Abstract

Despite the importance of sex-specific information for sturgeon conservation and management, sex identification has been a major challenge outside of mature adults on spawning grounds. Recent work identified a sex-specific locus (AllWSex2) that appears to be broadly conserved across many Acipenserids, but the assay was not validated for all species within the family. We tested the AllWSex2 marker in three sturgeon taxa (shortnose sturgeon Acipenser brevirostrum, Gulf sturgeon A. oxyrhinchus desotoi, and Atlantic sturgeon A. oxyrhinchus oxyrhinchus) from the Atlantic and Gulf of Mexico Coasts of North America to validate its use for sex identification. Our results indicate AllWSex2 is conserved in all three taxa, presenting a new opportunity to derive sex-specific information from tissue samples, which are routinely collected from these taxa. We found high concordance (range: 97–100%) between genotypic and phenotypic/histological methods, suggesting the assay is broadly effective. However, the small amount of discordance between the methods (< 3%) suggests further refinement may be possible.

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北美东部三种鲟鱼分子性别标记的验证

摘要尽管性别特异性信息对于鲟鱼的保护和管理非常重要，但在产卵场成熟成鱼之外的性别鉴定一直是一个重大挑战。最近的研究发现了一个性别特异性位点（AllWSex2），该位点似乎在许多鲟科鱼类中具有广泛的保守性，但该检测方法并未在鲟科的所有物种中得到验证。我们在北美大西洋和墨西哥湾沿岸的三个鲟鱼类群（短吻鲟Acipenser brevirostrum、海湾鲟A. oxyrhinchus desotoi和大西洋鲟A. oxyrhinchus oxyrhinchus）中测试了AllWSex2标记，以验证其在性别鉴定中的应用。我们的研究结果表明，AllWSex2 在所有这三个类群中都是保守的，这为从这些类群常规采集的组织样本中获取性别特异性信息提供了一个新的机会。我们发现基因型和表型/组织学方法之间的一致性很高（范围：97-100%），这表明该检测方法具有广泛的有效性。然而，两种方法之间存在的少量不一致（3%）表明有可能进一步改进。

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来源期刊

Conservation Genetics Resources BIODIVERSITY CONSERVATION-GENETICS & HEREDITY

CiteScore

2.90

自引率

9.10%

发文量

审稿时长

3-6 weeks

期刊介绍： Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.