Study of bacteriological profile and antibiotic susceptibility pattern of pus isolates in tertiary care hospital

Vijetha Sajjanar, Premalatha De, Siddesh Kc, Prakash N
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Abstract

Surgical wound causes invasion of pathogens causing surgical site infections which are commonly polymicrobial in nature. This pus forming infection causes delayed wound healing, wound dehiscence and wound breakdown contributing to important healthcare associated infections (HAI) Multidrug resistance has emerged among organisms isolated in pus sample due to failure of appropriate use of antibiotics.: 1. To study the bacteriological profile of pus samples 2. To determine the antibiotic susceptibility pattern of isolated pathogens from pus samples.The study was conducted in department of microbiology, Shimoga institute of medical sciences, Shimoga from January 2018 to June 2018.All pus samples were processed on blood agar, MacConkey agar and incubated at 37°c under aerobic conditions for 24 hours. The organisms were identified as per standard conventional methods. The antimicrobial susceptibility tests were done by Kirby–Bauer’s Disk Diffusion method on Mueller–Hinton Agar and interpreted as per clinical laboratory standard institution guidelines (CLSI). Out of 350 samples 250 were culture positive 100 were culture negative. Among culture positive most common organism isolated was followed by 86(34.45%), 72(28.8%), 55(22%), 20(8%), 9(3.6%), 2(0.8%), 3(1.2%) 3(1.2%), 3(1.2%). Gram positive organisms were most sensitive for linezolid, vancomycin and least sensitive to cefoxitin, erythromycin. Gram Negative Organisms Were Most Sensitive for Imipenam, Piperacillin tazobactam and least Sensitive for Ampicillin-sulbactam, Ciprofloxacin is most common etiology of pus forming infection most importantly surgical site infections (SSI). MRSA prevalence in hospital set up indicates the failure of proper infection control practices implementation in the hospitals causing healthcare associated infections (HAI). Emergence of multidrug resistance among the pus isolates is because of non-judicious use of antibiotics.
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三级护理医院脓液分离菌的细菌学特征和抗生素敏感性模式研究
手术伤口会导致病原体入侵,引起手术部位感染,这种感染通常是多菌性的。这种化脓性感染会导致伤口延迟愈合、伤口开裂和伤口破裂,从而引发重要的医疗相关感染(HAI):1.研究脓液样本的细菌学特征 2.该研究于 2018 年 1 月至 2018 年 6 月在希莫加医学科学研究所微生物学系进行。所有脓液样本均在血琼脂和麦康凯琼脂上处理,并在 37°c 有氧条件下培养 24 小时。按照标准常规方法对微生物进行鉴定。抗菌药敏感性测试采用柯比-鲍尔盘扩散法在穆勒-欣顿琼脂上进行,并根据临床实验室标准机构指南(CLSI)进行解释。在 350 份样本中,250 份培养呈阳性,100 份培养呈阴性。在培养呈阳性的样本中,最常见的分离菌依次为:86(34.45%)、72(28.8%)、55(22%)、20(8%)、9(3.6%)、2(0.8%)、3(1.2%)、3(1.2%)。革兰氏阳性菌对利奈唑胺、万古霉素最敏感,对头孢西丁、红霉素最不敏感。革兰氏阴性菌对亚胺培南、哌拉西林-他唑巴坦最敏感,对氨苄西林-舒巴坦最不敏感。MRSA 在医院中的流行表明,医院没有实施适当的感染控制措施,导致了医疗相关感染(HAI)。脓液分离物中出现多种药物耐药性的原因是抗生素使用不当。
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