Pub Date : 2024-04-15DOI: 10.18231/j.ijmmtd.2024.007
Partha Pratim Das, Chandra Jyoti Bora, Rashmi Ahmed, Hema Bahety
: Health care associated infections commonly encountered in paediatric intensive care units (PICU) are respiratory tract infections, and bloodstream infections. Monitoring of prevalence rates and antimicrobial susceptibility of different pathogens is necessary for proper management of PICU infections. : This study aimed to determine the microbial profile causing infections in patients admitted to PICU and their antimicrobial susceptibility pattern.: A cross sectional study was conducted involving all paediatric patients admitted in PICU during a 19 months period. Specimen viz. blood culture, urine, and tracheal aspirate were sent for culture and sensitivity and results were observed and their antibiotic susceptibility pattern was noted. : Out of total 372 patients, 72 (19.35%) were found culture positive. The isolation rate was very high in tracheal aspirates (82.14%) as compared to blood (14.29%) and urine (14.21%). Gram negative bacilli (76.3%) were the most common pathogen group isolated, (30.5%) commonest followed by species (19.4%). species (13.8%) was the most common gram positive isolate followed by Methicillin resistant (MRSA) (6.94%). Higher susceptibility was observed to Tigecycline & Meropenem among gram negative isolates while Linezolid and Vancomycin were most susceptible to gram positive cocci.: Gram negative bacteria were the predominant pathogens mostly species while species were the most common among Gram positive bacteria. Isolates showed multiple drug resistance to commonly used antimicrobials- Cephalosporins and Fluoroquinolones etc.
{"title":"Microbial profile and antimicrobial susceptibility pattern in paediatric intensive care unit of a tertiary care hospital, North East India","authors":"Partha Pratim Das, Chandra Jyoti Bora, Rashmi Ahmed, Hema Bahety","doi":"10.18231/j.ijmmtd.2024.007","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2024.007","url":null,"abstract":": Health care associated infections commonly encountered in paediatric intensive care units (PICU) are respiratory tract infections, and bloodstream infections. Monitoring of prevalence rates and antimicrobial susceptibility of different pathogens is necessary for proper management of PICU infections. : This study aimed to determine the microbial profile causing infections in patients admitted to PICU and their antimicrobial susceptibility pattern.: A cross sectional study was conducted involving all paediatric patients admitted in PICU during a 19 months period. Specimen viz. blood culture, urine, and tracheal aspirate were sent for culture and sensitivity and results were observed and their antibiotic susceptibility pattern was noted. : Out of total 372 patients, 72 (19.35%) were found culture positive. The isolation rate was very high in tracheal aspirates (82.14%) as compared to blood (14.29%) and urine (14.21%). Gram negative bacilli (76.3%) were the most common pathogen group isolated, (30.5%) commonest followed by species (19.4%). species (13.8%) was the most common gram positive isolate followed by Methicillin resistant (MRSA) (6.94%). Higher susceptibility was observed to Tigecycline & Meropenem among gram negative isolates while Linezolid and Vancomycin were most susceptible to gram positive cocci.: Gram negative bacteria were the predominant pathogens mostly species while species were the most common among Gram positive bacteria. Isolates showed multiple drug resistance to commonly used antimicrobials- Cephalosporins and Fluoroquinolones etc.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"321 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140703502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-15DOI: 10.18231/j.ijmmtd.2024.008
Kalpesh Khutade, Samiksha Patil, Harshada Shah, Hiren Patel
Tuberculosis (TB) is an infectious disease that can affect various parts of the body, with lung infections being the most common cause. In this study, drug susceptibility testing (DST) using LJ media was compared to Truenat testing to detect rifampicin resistance in sputum smear-positive cases with related clinical characteristics. The Vedantaa Institute of Medical Sciences conducted a laboratory-based study from July 2023 to December 2023, enrolling 102 clinical isolates. Sputum smears with acid-fast bacilli were cultured in LJ medium, isolated, and grown with rifampicin for resistance observation and a correlation with Truenat.The study found that individuals aged 21–30 had the highest prevalence of TB. The highest detection ratio was shown in the Below Poverty Line (BPL) at 84.2%, and Rifampicin (RIF) resistance was detected at 75%. A TB positive ratio of 68.4% were found to be both alcoholic and smoker population and 42.1% were found to have asthma and liver disease, no Rifampicin (RIF) resistance was detected in both the population. In HIV patients 50% RIF resistance was detected. MTB coinfections were observed in 68.4% of pneumonia patients. The LJ culture test had 95% sensitivity and 100% specificity for MTB detection, while the Truenat test had 100% sensitivity and 100% specificity for both MTB testing and MTB/RIF resistance detection.Demographic, clinical, and social variables, including alcoholics and smokers, asthma and liver disease, hypertension, diabetes, and co-infection with pneumonia, were the main factors for pulmonary tuberculosis patients. Micro-real-time PCR has higher clinical sensitivity for MTB detection, while conventional tests predict rifampicin resistance.
{"title":"The diagnostic concordance between micro real-time PCR and Lowenstein Jensen (LJ) media assays for pulmonary tuberculosis detection with associated clinical characteristics","authors":"Kalpesh Khutade, Samiksha Patil, Harshada Shah, Hiren Patel","doi":"10.18231/j.ijmmtd.2024.008","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2024.008","url":null,"abstract":"Tuberculosis (TB) is an infectious disease that can affect various parts of the body, with lung infections being the most common cause. In this study, drug susceptibility testing (DST) using LJ media was compared to Truenat testing to detect rifampicin resistance in sputum smear-positive cases with related clinical characteristics. The Vedantaa Institute of Medical Sciences conducted a laboratory-based study from July 2023 to December 2023, enrolling 102 clinical isolates. Sputum smears with acid-fast bacilli were cultured in LJ medium, isolated, and grown with rifampicin for resistance observation and a correlation with Truenat.The study found that individuals aged 21–30 had the highest prevalence of TB. The highest detection ratio was shown in the Below Poverty Line (BPL) at 84.2%, and Rifampicin (RIF) resistance was detected at 75%. A TB positive ratio of 68.4% were found to be both alcoholic and smoker population and 42.1% were found to have asthma and liver disease, no Rifampicin (RIF) resistance was detected in both the population. In HIV patients 50% RIF resistance was detected. MTB coinfections were observed in 68.4% of pneumonia patients. The LJ culture test had 95% sensitivity and 100% specificity for MTB detection, while the Truenat test had 100% sensitivity and 100% specificity for both MTB testing and MTB/RIF resistance detection.Demographic, clinical, and social variables, including alcoholics and smokers, asthma and liver disease, hypertension, diabetes, and co-infection with pneumonia, were the main factors for pulmonary tuberculosis patients. Micro-real-time PCR has higher clinical sensitivity for MTB detection, while conventional tests predict rifampicin resistance.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"64 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140699053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-15DOI: 10.18231/j.ijmmtd.2024.014
Heena Tak, Bhumika Chandra Lali, Tabassum Amber
This case report describes the presentation, investigation, and diagnosis of algaemia ( algaemia) in an immunocompromised patient with hematological malignancy. A patient of acute lymphoblastic leukemia was admitted with febrile neutropenia after a few weeks of induction chemotherapy. During hospital stay, the patient developed severe body pain, loss of appetite and persistent fever after a week of recovery. Upon thorough investigation, the patient was found to have central line related bloodstream infection with an emerging opportunistic environmental pathogen of genus species , sensitive to Amphotericin B, Fluconazole, Voriconazole, Micafungin and Caspofungin. Liposomal Amphotericin B, an antifungal treatment was prescribed, post-treatment blood cultures were negative.
本病例报告描述了一名免疫功能低下的血液恶性肿瘤患者的藻血症（algaemia）表现、调查和诊断。一名急性淋巴细胞白血病患者在接受了几周的诱导化疗后，因发热性中性粒细胞减少症入院。住院期间，患者全身剧烈疼痛，食欲不振，一周后康复，但仍持续发热。经过彻底检查，发现患者患有与中心管路相关的血流感染，感染的病原体是一种新出现的机会性环境病原体，属种，对两性霉素 B、氟康唑、伏立康唑、米卡芬净和卡泊芬净敏感。患者接受了两性霉素 B 脂质体抗真菌治疗，治疗后血液培养呈阴性。
{"title":"A case report of bloodstream infection by Prototheca zopfii: An emerging opportunistic pathogen","authors":"Heena Tak, Bhumika Chandra Lali, Tabassum Amber","doi":"10.18231/j.ijmmtd.2024.014","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2024.014","url":null,"abstract":"This case report describes the presentation, investigation, and diagnosis of algaemia ( algaemia) in an immunocompromised patient with hematological malignancy. A patient of acute lymphoblastic leukemia was admitted with febrile neutropenia after a few weeks of induction chemotherapy. During hospital stay, the patient developed severe body pain, loss of appetite and persistent fever after a week of recovery. Upon thorough investigation, the patient was found to have central line related bloodstream infection with an emerging opportunistic environmental pathogen of genus species , sensitive to Amphotericin B, Fluconazole, Voriconazole, Micafungin and Caspofungin. Liposomal Amphotericin B, an antifungal treatment was prescribed, post-treatment blood cultures were negative.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"51 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140701174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-15DOI: 10.18231/j.ijmmtd.2024.013
Ajay Lucas, Komali Jonnalagadda, Afwaan Faizal
This case report highlights the diagnostic intricacies and clinical implications of pelvic hydatidosis, particularly in the context of the Indian healthcare system. Despite being a rare occurrence, pelvic hydatidosis presents significant challenges in diagnosis and management, necessitating a comprehensive approach for optimal patient care. The case involved a 54-year-old male presenting with right thigh pain and a gradually enlarging groin and thigh swelling. Imaging studies revealed a large cystic lesion with multiple internal cysts extending into the pelvic cavity, suggestive of pelvic hydatidosis. Following confirmation of pelvic hydatidosis, the patient received a 7-day course of albendazole prior to undergoing surgical excision via laparotomy. The cystic mass located in the right thigh's medial aspect was successfully removed, with subsequent peritoneal irrigation and uneventful postoperative recovery. Oral albendazole was prescribed for 6 months, and at the 6-month follow-up, no signs of disease recurrence were observed. Histopathological examination confirmed the diagnosis, showcasing characteristic features of hydatid cysts. The rarity of pelvic hydatidosis compared to other forms of echinococcosis underscores the importance of maintaining a high index of suspicion, especially in regions where the disease is endemic. The complex socioeconomic landscape in India, characterized by poor sanitation and limited healthcare access, contributes to delayed diagnosis and increased morbidity rates.
{"title":"A rare case of pelvic hydatidosis","authors":"Ajay Lucas, Komali Jonnalagadda, Afwaan Faizal","doi":"10.18231/j.ijmmtd.2024.013","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2024.013","url":null,"abstract":"This case report highlights the diagnostic intricacies and clinical implications of pelvic hydatidosis, particularly in the context of the Indian healthcare system. Despite being a rare occurrence, pelvic hydatidosis presents significant challenges in diagnosis and management, necessitating a comprehensive approach for optimal patient care. The case involved a 54-year-old male presenting with right thigh pain and a gradually enlarging groin and thigh swelling. Imaging studies revealed a large cystic lesion with multiple internal cysts extending into the pelvic cavity, suggestive of pelvic hydatidosis. Following confirmation of pelvic hydatidosis, the patient received a 7-day course of albendazole prior to undergoing surgical excision via laparotomy. The cystic mass located in the right thigh's medial aspect was successfully removed, with subsequent peritoneal irrigation and uneventful postoperative recovery. Oral albendazole was prescribed for 6 months, and at the 6-month follow-up, no signs of disease recurrence were observed. Histopathological examination confirmed the diagnosis, showcasing characteristic features of hydatid cysts. The rarity of pelvic hydatidosis compared to other forms of echinococcosis underscores the importance of maintaining a high index of suspicion, especially in regions where the disease is endemic. The complex socioeconomic landscape in India, characterized by poor sanitation and limited healthcare access, contributes to delayed diagnosis and increased morbidity rates.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"53 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140699243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-15DOI: 10.18231/j.ijmmtd.2024.001
Santosh Kotgire
{"title":"Molecular epidemology","authors":"Santosh Kotgire","doi":"10.18231/j.ijmmtd.2024.001","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2024.001","url":null,"abstract":"","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"45 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140701561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-15DOI: 10.18231/j.ijmmtd.2024.002
A. Al-Haddad, Y. M. Matran, Abdullah O. Ba Omer
() infections among children have become an alarming challenge for developed and developing countries. This narrative review highlights the concerning situation of and its antimicrobial resistance among Middle Eastern children. The review targeted studies on infections in the Middle East, focusing on those publications that were conducted within the past 14 years. The selected publications were sourced from the Google Scholar and PubMed databases. There was scanty published data addressing compylobacteriosis and its antimicrobial profile specifically among Middle Eastern children. Although there has been international concern about the role of and in causing childhood diarrhea, many regions in the Middle East do not appear to share the same level of concern. Furthermore, the complex interplay of socio-demographic and environmental elements that may influence infections, coupled with the globally rising concern over antibiotic resistance in spp (particularly multidrug-resistant strains), raises significant concerns in this region. Moreover, the presence of co-infections with , known to worsen disease severity, could exacerbate the issue and place a greater burden on healthcare systems. Additionally, the low awareness and insufficient prioritization of this public health issue, compounded by political conflicts in parts of the Middle East, present multiple challenges for children in several regions of the Middle East. These challenges, along with the consequences of late sequelae from infection, put many children in this region at significant risk. To effectively tackle these challenges, critical targeted interventions must be implemented. These interventions include conducting comprehensive epidemiological studies to understand the burden of in Middle Eastern children, characterizing the antibiotic susceptibility patterns of prevalent strains, updating healthcare practices to align with prevailing resistance profiles, adopting appropriate antibiotic prescription policies, and implementing robust infection control measures in this specific population.
(儿童（）感染已成为发达国家和发展中国家面临的一个令人担忧的挑战。这篇叙事性综述着重介绍了中东地区儿童的感染情况及其抗菌药耐药性。综述以中东地区的感染研究为目标，重点关注过去 14 年内发表的出版物。所选出版物来自 Google Scholar 和 PubMed 数据库。针对中东儿童的双球菌病及其抗菌谱的发表数据很少。尽管国际社会一直在关注和引起儿童腹泻的原因，但中东的许多地区似乎并没有同样的关注。此外，可能影响感染的社会人口和环境因素之间存在复杂的相互作用，再加上全球范围内对抗生素耐药性（尤其是耐多药菌株）的日益关注，这些都引起了该地区的高度关注。此外，众所周知，合并感染会加重疾病的严重程度，这可能会加剧这一问题，并给医疗保健系统带来更大的负担。此外，对这一公共卫生问题的认识不足和重视不够，再加上中东部分地区的政治冲突，给中东一些地区的儿童带来了多重挑战。这些挑战以及感染后遗症的后果使该地区的许多儿童面临巨大风险。为了有效应对这些挑战，必须实施关键的针对性干预措施。这些干预措施包括开展全面的流行病学研究以了解中东儿童的感染负担、确定流行菌株的抗生素敏感性模式、更新医疗保健实践以适应当前的耐药性概况、采取适当的抗生素处方政策以及在这一特定人群中实施强有力的感染控制措施。
{"title":"Campylobacter infections in Middle Eastern children: Review article","authors":"A. Al-Haddad, Y. M. Matran, Abdullah O. Ba Omer","doi":"10.18231/j.ijmmtd.2024.002","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2024.002","url":null,"abstract":"() infections among children have become an alarming challenge for developed and developing countries. This narrative review highlights the concerning situation of and its antimicrobial resistance among Middle Eastern children. The review targeted studies on infections in the Middle East, focusing on those publications that were conducted within the past 14 years. The selected publications were sourced from the Google Scholar and PubMed databases. There was scanty published data addressing compylobacteriosis and its antimicrobial profile specifically among Middle Eastern children. Although there has been international concern about the role of and in causing childhood diarrhea, many regions in the Middle East do not appear to share the same level of concern. Furthermore, the complex interplay of socio-demographic and environmental elements that may influence infections, coupled with the globally rising concern over antibiotic resistance in spp (particularly multidrug-resistant strains), raises significant concerns in this region. Moreover, the presence of co-infections with , known to worsen disease severity, could exacerbate the issue and place a greater burden on healthcare systems. Additionally, the low awareness and insufficient prioritization of this public health issue, compounded by political conflicts in parts of the Middle East, present multiple challenges for children in several regions of the Middle East. These challenges, along with the consequences of late sequelae from infection, put many children in this region at significant risk. To effectively tackle these challenges, critical targeted interventions must be implemented. These interventions include conducting comprehensive epidemiological studies to understand the burden of in Middle Eastern children, characterizing the antibiotic susceptibility patterns of prevalent strains, updating healthcare practices to align with prevailing resistance profiles, adopting appropriate antibiotic prescription policies, and implementing robust infection control measures in this specific population.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"9 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140698871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-15DOI: 10.18231/j.ijmmtd.2024.004
Md. Mudassar Iqbal Lodhi, Lakshminarayana S A, Aaftab G.P
The continuous rising of hospital acquired Multi Drug resistant creates an alarming condition in public health worldwide. Objective of this study is to identify quantitative analysis of hospital acquired Multi-Drug resistant in India. Data were collected from Pub Med and Google scholar specific study of India to identify prevalence of Multi-drug resistant nosocomial starting from 2011 to 2023. Literature review were collected and analysed through Preferred reporting Items for Systematic Reviews and meta-analysis (PRISMA) guideline and SPSS- 25 software used for Data analysis. Meta-analysis report reveals that prevalence of multi-drug resistant among hospital acquired is 34.37 % and pooled prevalence rate of hospital acquired multi drug resistant infection found at 2 % (97.5 % CI, 2.5) with p-value is 0.01. Genotypic analysis reveals bla gene identified most among the studied samples. Prevalence of ESBL, MDR associated with nosocomial infections estimated and correlated with mortality and death ratio as well as antibiotic susceptibility tests pattern was analysed in India which shows continuous rising number of MDR and ESBL especially hospital acquired infection. A finding of this study is indicating alarming situation of public health and it should control through interdisciplinary one health approach.
{"title":"Occurrence of nosocomial multi-drug resistant Klebsiella pneumoniae in India: A systemic review and meta-analysis","authors":"Md. Mudassar Iqbal Lodhi, Lakshminarayana S A, Aaftab G.P","doi":"10.18231/j.ijmmtd.2024.004","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2024.004","url":null,"abstract":"The continuous rising of hospital acquired Multi Drug resistant creates an alarming condition in public health worldwide. Objective of this study is to identify quantitative analysis of hospital acquired Multi-Drug resistant in India. Data were collected from Pub Med and Google scholar specific study of India to identify prevalence of Multi-drug resistant nosocomial starting from 2011 to 2023. Literature review were collected and analysed through Preferred reporting Items for Systematic Reviews and meta-analysis (PRISMA) guideline and SPSS- 25 software used for Data analysis. Meta-analysis report reveals that prevalence of multi-drug resistant among hospital acquired is 34.37 % and pooled prevalence rate of hospital acquired multi drug resistant infection found at 2 % (97.5 % CI, 2.5) with p-value is 0.01. Genotypic analysis reveals bla gene identified most among the studied samples. Prevalence of ESBL, MDR associated with nosocomial infections estimated and correlated with mortality and death ratio as well as antibiotic susceptibility tests pattern was analysed in India which shows continuous rising number of MDR and ESBL especially hospital acquired infection. A finding of this study is indicating alarming situation of public health and it should control through interdisciplinary one health approach.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"60 S10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140702514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-15DOI: 10.18231/j.ijmmtd.2024.005
R. Procópio, D. B. Pereira, J. G. Martins, Yohonatan Alain Duque Aurazo
Scorpion venoms contain different classes of molecules with possible pharmacological activities, making them sources of bioactive molecules for the development of new drugs against infections caused by pathogens, such as malaria, a disease caused by protozoa of the genus Plasmodium. Malaria faces challenges in its control due to pathogen resistance to available antimalarials. In this study, we evaluated the venom activity of the Amazonian scorpions and against FRC3, the analysis was performed by flow cytometry. At the analyzed concentrations, we found that the crude venom of had an average inhibition of 87% at the concentration of 100 µg/mL, above that obtained with the drug (quinine), which had mean inhibition of 84% against FCR3. Regarding the venom of , lower activity was observed in comparison with the inhibition potential of the venom and the standard drug, venom showed low toxicity against the human fibroblast MRC5. Because peptides and toxins from scorpion venom are related to biological functions, they can be used in the design of new therapeutic agents, with venom being a possible source of molecules for the development of antimalarial drugs.
{"title":"In vitro activity of Tityus metuendus and Brotheas amazonicus scorpion venoms against Plasmodium falciparum FRC3","authors":"R. Procópio, D. B. Pereira, J. G. Martins, Yohonatan Alain Duque Aurazo","doi":"10.18231/j.ijmmtd.2024.005","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2024.005","url":null,"abstract":"Scorpion venoms contain different classes of molecules with possible pharmacological activities, making them sources of bioactive molecules for the development of new drugs against infections caused by pathogens, such as malaria, a disease caused by protozoa of the genus Plasmodium. Malaria faces challenges in its control due to pathogen resistance to available antimalarials. In this study, we evaluated the venom activity of the Amazonian scorpions and against FRC3, the analysis was performed by flow cytometry. At the analyzed concentrations, we found that the crude venom of had an average inhibition of 87% at the concentration of 100 µg/mL, above that obtained with the drug (quinine), which had mean inhibition of 84% against FCR3. Regarding the venom of , lower activity was observed in comparison with the inhibition potential of the venom and the standard drug, venom showed low toxicity against the human fibroblast MRC5. Because peptides and toxins from scorpion venom are related to biological functions, they can be used in the design of new therapeutic agents, with venom being a possible source of molecules for the development of antimalarial drugs.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"39 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140699129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-15DOI: 10.18231/j.ijmmtd.2024.010
Swapan Banerjee, Urvashi Sharma, Virender Pal Kaur
Purpose: Dengue is one of the common public health issues globally where laboratory diagnostic test values of patients are primarily focused on hematological parameters. Micronutrients equally take a role in quick recovery, and active cum passive immunity is not mainly considered during and post-recovery of patients. Materials and Methods: A cross-sectional study with a purposive sampling method was applied using pre-tested close-ended questionnaires and laboratory data (soft copies/hard copies) of participants admitted to hospitals or nursing homes within the North -24 Paraganas district from November 2021 to October 2022. 110 male and female patients of 40-60 years reported dengue fever (non-hospitalized cases), and dengue hemorrhagic fever (hospitalized cases) was considered based on 15 variables. STATA version 18.0 software analyzed data using multivariate regression and range plots. The Cronbach’s alpha value was 0.72, and the consolidated probability value shows 0.01 (p<0.05), which denotes that the multivariate model was statistically significant and was rightly decided to include the relevant independent variables. Calcium, vitamin D, and total protein were statistically significant as P values were 0.01, the same for each. Similarly, p=0.05 was the same for Total Leukocyte count (TLC) and Alkaline phosphatase (ALP).The study concludes that micronutrients like calcium, protein, vitamin D, etc., are often considered secondary recommendations in dengue treatment. A balanced, nutrient-rich diet for dengue patients is needed to maintain sustained immunity by extensively monitoring calcium, vitamin D, and total protein intake during and after recovery.
目的：登革热是全球常见的公共卫生问题之一，患者的实验室诊断测试值主要集中在血液学参数上。微量营养素在快速康复中同样发挥着作用，而主动免疫和被动免疫在患者康复期间和康复后并未得到主要考虑。材料和方法：使用预先测试的封闭式调查问卷和实验室数据（软拷贝/硬拷贝），对 2021 年 11 月至 2022 年 10 月期间在北帕拉加纳斯区（North -24 Paraganas）的医院或疗养院住院的参与者进行有目的抽样的横断面研究。110 名 40-60 岁的男女患者报告了登革热（非住院病例），并根据 15 个变量考虑了登革出血热（住院病例）。STATA 18.0 版软件使用多元回归和范围图分析数据。Cronbach'sα值为0.72，综合概率值显示为0.01（P<0.05），这表明多变量模型具有统计学意义，并正确地决定将相关自变量纳入其中。钙、维生素 D 和总蛋白质的 P 值均为 0.01，具有统计学意义。同样，总白细胞计数（TLC）和碱性磷酸酶（ALP）的 P 值均为 0.05。研究得出结论，钙、蛋白质、维生素 D 等微量营养素通常被视为登革热治疗的次要建议。登革热患者需要均衡、营养丰富的饮食，以维持持续的免疫力，在康复期间和康复后广泛监测钙、维生素 D 和总蛋白的摄入量。
{"title":"Analysis of essential hematological parameters and nutrient levels of dengue patients aiming for quick recovery and sustainable immunity","authors":"Swapan Banerjee, Urvashi Sharma, Virender Pal Kaur","doi":"10.18231/j.ijmmtd.2024.010","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2024.010","url":null,"abstract":"Purpose: Dengue is one of the common public health issues globally where laboratory diagnostic test values of patients are primarily focused on hematological parameters. Micronutrients equally take a role in quick recovery, and active cum passive immunity is not mainly considered during and post-recovery of patients. Materials and Methods: A cross-sectional study with a purposive sampling method was applied using pre-tested close-ended questionnaires and laboratory data (soft copies/hard copies) of participants admitted to hospitals or nursing homes within the North -24 Paraganas district from November 2021 to October 2022. 110 male and female patients of 40-60 years reported dengue fever (non-hospitalized cases), and dengue hemorrhagic fever (hospitalized cases) was considered based on 15 variables. STATA version 18.0 software analyzed data using multivariate regression and range plots. The Cronbach’s alpha value was 0.72, and the consolidated probability value shows 0.01 (p<0.05), which denotes that the multivariate model was statistically significant and was rightly decided to include the relevant independent variables. Calcium, vitamin D, and total protein were statistically significant as P values were 0.01, the same for each. Similarly, p=0.05 was the same for Total Leukocyte count (TLC) and Alkaline phosphatase (ALP).The study concludes that micronutrients like calcium, protein, vitamin D, etc., are often considered secondary recommendations in dengue treatment. A balanced, nutrient-rich diet for dengue patients is needed to maintain sustained immunity by extensively monitoring calcium, vitamin D, and total protein intake during and after recovery.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"44 26","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140701775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-15DOI: 10.18231/j.ijmmtd.2024.009
Sweta Singh, C. Sahu, Akanksha Dubey, U. Ghoshal
: The rise of cases of the infectious COVID -19 pandemic has led to significant burden on healthcare settings. : The current study tried to evaluate the knowledge, perceptions and experiences of Health care workers (HCW) towards a well-executed PPE and Hand Hygiene (HH) training before and after attending the sessions : Study was planned for a period of twelve months for various HCWs of different departments of the Institute. Every participant was evaluated for pre-test as well as post-test knowledge (cognitive domain) assessment using a pre-structured format along with demonstration (psychomotor domain). : A total of 535 HCWs were included for the study. Statistically significant (P < 0.001) increase in post test score was noted in different domains of learning of Faculty, residents and Nurses group. The mean pre-test and post test score was significantly higher for Critical care units (CCU) as compared to other units. The difference in overall scores for various parameters in pre and post training was as follows: 5.2 for MCQs,2.5 for PPE OSPE,5.1 for HH OSPE and13.1 for total scores. : HH and PPE training sessions will impart knowledge and confidence to HCWs working in hospitals for better patient care as well as infection control practices.
{"title":"Personal protective equipment (PPE) and Hand hygiene training sessions: Knowledge and perceptions in the current COVID pandemic","authors":"Sweta Singh, C. Sahu, Akanksha Dubey, U. Ghoshal","doi":"10.18231/j.ijmmtd.2024.009","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2024.009","url":null,"abstract":": The rise of cases of the infectious COVID -19 pandemic has led to significant burden on healthcare settings. : The current study tried to evaluate the knowledge, perceptions and experiences of Health care workers (HCW) towards a well-executed PPE and Hand Hygiene (HH) training before and after attending the sessions : Study was planned for a period of twelve months for various HCWs of different departments of the Institute. Every participant was evaluated for pre-test as well as post-test knowledge (cognitive domain) assessment using a pre-structured format along with demonstration (psychomotor domain). : A total of 535 HCWs were included for the study. Statistically significant (P < 0.001) increase in post test score was noted in different domains of learning of Faculty, residents and Nurses group. The mean pre-test and post test score was significantly higher for Critical care units (CCU) as compared to other units. The difference in overall scores for various parameters in pre and post training was as follows: 5.2 for MCQs,2.5 for PPE OSPE,5.1 for HH OSPE and13.1 for total scores. : HH and PPE training sessions will impart knowledge and confidence to HCWs working in hospitals for better patient care as well as infection control practices.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"80 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140702686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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