Lipid-lowering capacity of GABA-rich supernatant from fermented okara in OA-induced HepG2 cells

IF 4.8 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Food Bioscience Pub Date : 2024-01-28 DOI:10.1016/j.fbio.2024.103659
Yuyi Xiao , Ziqi Liu , Pengcheng Li , Yubo Wang , Xiujuan Wang , Chunhong Piao , Lihua Yuan
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Abstract

γ-Aminobutyric acid (GABA) has been shown to have many beneficial functions and has received widespread attention. To broaden the application of GABA-rich fermentation supernatant, this study investigated the lipid-lowering capacity of GABA-rich supernatant from fermented okara (FOS-G) in vitro. The results showed that FOS-G improved the oxidative stress in OA-induced HepG2 cells by reducing the content of reactive oxygen species (ROS) and malondialdehyde (MDA). Furthermore, FOS-G improved the lipid accumulation in cells by reducing total cholesterol (TC) and triglyceride (TG). The results of Western blot showed that FOS-G exerted an ameliorative effect by activating the Keap1/Nrf2/HO-1 pathway and AMPK/SREBP-1 pathway. In particular, adipogenesis was inhibited by activating AMPK phosphorylation. The results showed that the therapeutic properties of FOS-G were directly related to its GABA content. In summary, FOS-G has a lipid-lowering capacity and can be used as a functional food ingredient for improving hyperlipidemia and non-alcoholic fatty liver disease (NAFLD).

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发酵秋葵富含 GABA 的上清液在 OA 诱导的 HepG2 细胞中的降脂能力
γ-氨基丁酸(GABA)已被证明具有多种有益功能,并受到广泛关注。为了拓宽富含 GABA 的发酵上清液的应用范围,本研究在体外研究了富含 GABA 的发酵秋葵上清液(FOS-G)的降脂能力。结果表明,FOS-G 通过降低活性氧(ROS)和丙二醛(MDA)的含量,改善了 OA 诱导的 HepG2 细胞的氧化应激。此外,FOS-G 还能降低总胆固醇(TC)和甘油三酯(TG),从而改善细胞中的脂质积累。Western 印迹结果显示,FOS-G 通过激活 Keap1/Nrf2/HO-1 通路和 AMPK/SREBP-1 通路发挥了改善作用。尤其是通过激活 AMPK 磷酸化抑制了脂肪的生成。结果表明,FOS-G 的治疗特性与其 GABA 含量直接相关。总之,FOS-G 具有降脂能力,可用作改善高脂血症和非酒精性脂肪肝(NAFLD)的功能性食品配料。
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来源期刊
Food Bioscience
Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍: Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.
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