N Schoutteten, A Yurkov, O Leroux, D Haelewaters, D Van Der Straeten, O Miettinen, T Boekhout, D Begerow, A Verbeken
{"title":"Diversity of colacosome-interacting mycoparasites expands the understanding of the evolution and ecology of <i>Microbotryomycetes</i>.","authors":"N Schoutteten, A Yurkov, O Leroux, D Haelewaters, D Van Der Straeten, O Miettinen, T Boekhout, D Begerow, A Verbeken","doi":"10.3114/sim.2023.106.02","DOIUrl":null,"url":null,"abstract":"<p><p><b></b> Mycoparasites in <i>Basidiomycota</i> comprise a diverse group of fungi, both morphologically and phylogenetically. They interact with their hosts through either fusion-interaction or colacosome-interaction. Colacosomes are subcellular structures formed by the mycoparasite at the host-parasite interface, which penetrate the parasite and host cell walls. Previously, these structures were detected in 19 fungal species, usually by means of transmission electron microscopy. Most colacosome-forming species have been assigned to <i>Microbotryomycetes</i> (<i>Pucciniomycotina</i>, <i>Basidiomycota</i>), a highly diverse class, comprising saprobic yeasts, mycoparasites, and phytoparasites. In general, these myco- and phytoparasites are dimorphic organisms, with a parasitic filamentous morph and saprobic yeast morph. We investigated colacosome-forming mycoparasites based on fungarium material, freshly collected specimens, and cultures of yeast morphs. We characterised the micromorphology of filamentous morphs, the physiological characteristics of yeast morphs, and inferred phylogenetic relationships based on DNA sequence data from seven loci. We outline and employ an epifluorescence-based microscopic method to assess the presence and organisation of colacosomes. We describe five new species in the genus <i>Colacogloea</i>, the novel dimorphic mycoparasite <i>Mycogloiocolax gerardii</i>, and provide the first report of a sexual, mycoparasitic morph in <i>Colacogloea philyla</i> and in the genus <i>Slooffia</i>. We detected colacosomes in eight fungal species, which brings the total number of known colacosome-forming fungi to 27. Finally, we revealed three distinct types of colacosome organisation in <i>Microbotryomycetes</i>. <b>Taxonomic novelties and typifications:</b> <b>New family:</b> <i>Mycogloiocolacaeae</i> Schoutteten & Yurkov; <b>New genus:</b> <i>Mycogloiocolax</i> Schoutteten & Rödel; <b>New species:</b> <i>Colacogloea bettinae</i> Schoutteten & Begerow, <i>C. biconidiata</i> Schoutteten, <i>C. fennica</i> Schoutteten & Miettinen, <i>C. microspora</i> Schoutteten, <i>C. universitatis-gandavensis</i> Schoutteten & Verbeken, <i>Mycogloiocolax gerardii</i> Schoutteten & Rödel; <b>New combinations:</b> <i>Slooffia micra</i> (Bourdot & Galzin) Schoutteten, <i>Fellozyma cerberi</i> (A.M. Yurkov <i>et al.</i>) Schoutteten & Yurkov, <i>Fellozyma telluris</i> (A.M. Yurkov <i>et al.</i>) Schoutteten & Yurkov; <b>Epitypifications (basionyms):</b> <i>Achroomyces insignis</i> Hauerslev, <i>Platygloea micra</i> Bourdot & Galzin, <i>Platygloea peniophorae</i> Bourdot & Galzin; <b>Lectotypification (basionym):</b> <i>Platygloea peniophorae</i> Bourdot & Galzin <b>Citation:</b> Schoutteten N, Yurkov A, Leroux O, Haelewaters D, Van Der Straeten D, Miettinen O, Boekhout T, Begerow D, Verbeken A (2023). Diversity of colacosome-interacting mycoparasites expands the understanding of the evolution and ecology of <i>Microbotryomycetes</i>. <i>Studies in Mycology</i> <b>106</b>: 41-94. doi: 10.3114/sim.2022.106.02.</p>","PeriodicalId":22036,"journal":{"name":"Studies in Mycology","volume":null,"pages":null},"PeriodicalIF":14.1000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10825749/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Studies in Mycology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3114/sim.2023.106.02","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/7/19 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Mycoparasites in Basidiomycota comprise a diverse group of fungi, both morphologically and phylogenetically. They interact with their hosts through either fusion-interaction or colacosome-interaction. Colacosomes are subcellular structures formed by the mycoparasite at the host-parasite interface, which penetrate the parasite and host cell walls. Previously, these structures were detected in 19 fungal species, usually by means of transmission electron microscopy. Most colacosome-forming species have been assigned to Microbotryomycetes (Pucciniomycotina, Basidiomycota), a highly diverse class, comprising saprobic yeasts, mycoparasites, and phytoparasites. In general, these myco- and phytoparasites are dimorphic organisms, with a parasitic filamentous morph and saprobic yeast morph. We investigated colacosome-forming mycoparasites based on fungarium material, freshly collected specimens, and cultures of yeast morphs. We characterised the micromorphology of filamentous morphs, the physiological characteristics of yeast morphs, and inferred phylogenetic relationships based on DNA sequence data from seven loci. We outline and employ an epifluorescence-based microscopic method to assess the presence and organisation of colacosomes. We describe five new species in the genus Colacogloea, the novel dimorphic mycoparasite Mycogloiocolax gerardii, and provide the first report of a sexual, mycoparasitic morph in Colacogloea philyla and in the genus Slooffia. We detected colacosomes in eight fungal species, which brings the total number of known colacosome-forming fungi to 27. Finally, we revealed three distinct types of colacosome organisation in Microbotryomycetes. Taxonomic novelties and typifications:New family:Mycogloiocolacaeae Schoutteten & Yurkov; New genus:Mycogloiocolax Schoutteten & Rödel; New species:Colacogloea bettinae Schoutteten & Begerow, C. biconidiata Schoutteten, C. fennica Schoutteten & Miettinen, C. microspora Schoutteten, C. universitatis-gandavensis Schoutteten & Verbeken, Mycogloiocolax gerardii Schoutteten & Rödel; New combinations:Slooffia micra (Bourdot & Galzin) Schoutteten, Fellozyma cerberi (A.M. Yurkov et al.) Schoutteten & Yurkov, Fellozyma telluris (A.M. Yurkov et al.) Schoutteten & Yurkov; Epitypifications (basionyms):Achroomyces insignis Hauerslev, Platygloea micra Bourdot & Galzin, Platygloea peniophorae Bourdot & Galzin; Lectotypification (basionym):Platygloea peniophorae Bourdot & Galzin Citation: Schoutteten N, Yurkov A, Leroux O, Haelewaters D, Van Der Straeten D, Miettinen O, Boekhout T, Begerow D, Verbeken A (2023). Diversity of colacosome-interacting mycoparasites expands the understanding of the evolution and ecology of Microbotryomycetes. Studies in Mycology106: 41-94. doi: 10.3114/sim.2022.106.02.
期刊介绍:
The international journal Studies in Mycology focuses on advancing the understanding of filamentous fungi, yeasts, and various aspects of mycology. It publishes comprehensive systematic monographs as well as topical issues covering a wide range of subjects including biotechnology, ecology, molecular biology, pathology, and systematics. This Open-Access journal offers unrestricted access to its content.
Each issue of Studies in Mycology consists of around 5 to 6 papers, either in the form of monographs or special focused topics. Unlike traditional length restrictions, the journal encourages submissions of manuscripts with a minimum of 50 A4 pages in print. This ensures a thorough exploration and presentation of the research findings, maximizing the depth of the published work.