Effect of Syzygium cumini on Oxidative Stress Induced Cardiac Cellular Anomalies.

Renu Bhadana, Vibha Rani
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Abstract

Introduction: Doxorubicin (Dox), an antineoplastic agent is used as a primary anticancerous drug against various types of cancers. However, its associated toxicity to the cardiovascular system is major. Literature has recorded the cases of mortality due to poor validation and lack of prediagnosis of Dox-induced cardiotoxicity. Therapeutic interventions using natural products having cardioprotective properties with low toxic outcomes hold therapeutic potential for future cardio-oncological therapies. Syzygium cumini (Black berry), a traditional Indian herbal plant, has been researched and found to exert cardioprotective, anti-inflammatory, and antioxidant activities, which have been credited due to the presence of polyphenols, flavonoids, and tannins.

Methods: In the current research, we investigated the cardioprotective potential of Syzygium cumini against Doxorubicin-induced cardiotoxicity (DIC) in H9C2 cardiomyocytes. Methanolic seed extract preparation of Syzygium cumini was performed using the Soxhlet apparatus. Cell viability and cell death assays were performed to determine the cardiotoxic doses of Doxorubicin. Furthermore, the cardioprotective potential of Syzygium cumini extract against DIC was studied. Morphological and nuclear alterations in H9C2 cells were studied by microscopic assays using Giemsa, Haematoxylin-Eosin stain, and PI. The intracellular stress level and ROS production were studied using DCFH-DA followed by mitochondrial integrity analysis using fluorescent microscopic methods.

Results: In the results, we investigated that Dox exerted a dose and time-dependent cardiotoxicity on H9C2 cardiomyocytes. Moreover, we observed that morphological and nuclear alterations caused by doxorubicin in dose-dependent manner were prevented by supplementing with Syzygium cumini polyphenols and it attenuated the oxidative stress in H9C2 cardiomyocytes effectively.

Conclusion: Conclusively, Syzygium cumini possesses cardioprotective potential in H9C2 cardiomyocytes in dox-induced cardiotoxicity.

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茜草对氧化应激诱导的心脏细胞异常的影响
简介多柔比星(Dox)是一种抗肿瘤药物,是治疗各种癌症的主要抗癌药物。然而,它对心血管系统的毒性很大。文献中记录了一些由于对 Dox 引起的心脏毒性验证不足和缺乏预诊而导致死亡的病例。利用具有心脏保护特性且毒性低的天然产品进行治疗干预,对未来的心肿瘤疗法具有治疗潜力。对印度传统草药植物黑浆果(Syzygium cumini)进行了研究,发现它具有保护心脏、抗炎和抗氧化活性,这归功于其含有多酚、类黄酮和单宁酸:在本研究中,我们研究了茜草对 H9C2 心肌细胞中多柔比星诱导的心脏毒性(DIC)的心脏保护潜力。使用索氏提取器制备烟叶紫苏的甲醇种子提取物。通过细胞存活率和细胞死亡检测来确定多柔比星的心脏毒性剂量。此外,还研究了杜仲提取物对 DIC 的心脏保护潜力。通过使用革兰氏染色法、血栓素-伊红染色法和 PI 进行显微检测,研究了 H9C2 细胞的形态和核改变。使用 DCFH-DA 研究了细胞内应力水平和 ROS 的产生,然后使用荧光显微镜方法分析了线粒体的完整性:结果:我们研究发现,Dox 对 H9C2 心肌细胞具有剂量和时间依赖性的心脏毒性。此外,我们还观察到,补充紫叶女贞多酚后,多柔比星引起的形态学和细胞核改变呈剂量依赖性,而紫叶女贞多酚则能有效防止多柔比星引起的形态学和细胞核改变,并能有效减轻 H9C2 心肌细胞的氧化应激:结论:在多西环素诱导的心脏毒性中,紫锥菊对 H9C2 心肌细胞具有心脏保护潜力。
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