Targeting colon cancer via antimicrobial RT2 peptide: a system biology study.

Q3 Medicine Gastroenterology and Hepatology From Bed to Bench Pub Date : 2023-01-01 DOI:10.22037/ghfbb.v16i4.2695

Zahra Hosseinpour, Mona Zamanian Azodi, Somayeh Jahani Sherafat, Mostafa Rezaei Tavirani

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Abstract

Aim: This study aims to investigate the anticancer molecular mechanism of RT2 through protein-protein interaction (PPI) network analysis. For this aim, a bioinformatics evaluation of the proteome profile of colon cancer is carried out.

Background: Antimicrobial peptides such as RT2 showed anticancer properties against various tumors. The molecular mechanism of the anticancer effect of RT2 is a challenging subject.

Methods: By applying Cytoscape V.3.9.1 and integrated apps, the profile of the interaction network and related centrality is analyzed. An enrichment analysis of hub bottlenecks was also performed, and highlighted biological processes were visualized and determined.

Results: Several 207 differentially expressed proteins were retrieved by PPI network analysis, and 10 hub bottlenecks were introduced. Among these differentially expressed proteins (DEPs), only AKT1 is from the queried DEPs. Key biological processes contributing to RT2 targeting mechanism include "Regulation of fibroblast proliferation", "Positive regulation of cyclin-dependent protein serine/threonine kinase activity", "positive regulation of miRNA transcription", and "fungiform papilla formation".

Conclusion: In conclusion, central proteins Tp53, MYC, EGFR, AKT1, HDAC1, and SRC can be introduced as a targeted biomarker panel of bioactive peptide treatments. However, extensive research is required to establish this claim before clinical application.

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通过抗菌 RT2 肽靶向结肠癌：一项系统生物学研究。

目的：本研究旨在通过蛋白质-蛋白质相互作用（PPI）网络分析研究RT2的抗癌分子机制。为此，研究人员对结肠癌的蛋白质组概况进行了生物信息学评估：背景：RT2 等抗菌肽对多种肿瘤具有抗癌作用。背景：RT2等抗菌肽对多种肿瘤具有抗癌作用，但RT2抗癌作用的分子机制是一个具有挑战性的课题：应用 Cytoscape V.3.9.1 和集成应用程序，分析了相互作用网络的概况和相关中心性。方法：应用 Cytoscape V.3.9.1 和集成的应用程序，分析了相互作用网络的概况和相关的中心性，还对中心瓶颈进行了富集分析，并对突出的生物过程进行了可视化和测定：结果：通过 PPI 网络分析检索到了 207 个差异表达蛋白，并引入了 10 个中心瓶颈。在这些差异表达蛋白（DEPs）中，只有 AKT1 来自查询到的 DEPs。RT2靶向机制的关键生物过程包括 "成纤维细胞增殖调节"、"细胞周期蛋白依赖性丝氨酸/苏氨酸激酶活性的正向调节"、"miRNA转录的正向调节 "和 "真菌乳头形成"：总之，中心蛋白 Tp53、MYC、表皮生长因子受体、AKT1、HDAC1 和 SRC 可作为生物活性肽治疗的靶向生物标记物。不过，在临床应用之前，还需要进行大量的研究来证实这一说法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Gastroenterology and Hepatology From Bed to Bench Medicine-Gastroenterology

CiteScore

2.30

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0.00%

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