Genetic Regulation of Interleukin-6 and Interleukin-10 in COVID-19 Infection.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-07-01 DOI:10.61186/rbmb.12.2.284
Zahra Rostami-Far, Khaled Rahmani, Kamran Mansouri, Mohammad Bagher Khadem Erfan, Farhad Shaveisi-Zadeh, Bahram Nikkhoo
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Abstract

Background: The role and regulation mechanisms of the interleukin-6 and 10 (IL6 and IL-10) serum levels and the interaction between CD4+ and CD8+ lymphocytes with SARS-COV-2 IgM and IgG in the context of COVID-19 infection are not fully understood.

Methods: This study was conducted on 45 COVID-19 patients and 45 healthy individuals. The IL-6 and IL-10 promoter methylation, IL-6 and IL-10 gene expression, SARS-COV-2 IgM, and IgG antibodies and CD4+ and CD8+ lymphocytes were studied by qMSP-PCR, Real-time PCR, ELISA, and flow cytometry techniques, respectively.

Results: The male ratio and mean age of critically ill patients' group were significantly higher in compared to controls (P< 0.05). IL-6 gene expression and serum levels were significantly increased in patients compared to controls (P=0.002, 0.001), but IL-6 promoter methylation was not significantly decreased in patients (P=0.835). The IL-10 promoter methylation and expression were not different between cases and controls (0.326, 0.455), but serum IL-10 levels were higher in patients (P< 0.001). The CD4+ and CD8+ lymphocytes decreased (P< 0.001) and mean SARS-COV-2 IgG increased (P=0.002) in the patients compared to controls.

Conclusions: The COVID-19 disease result in severe complications in men and elderly. The serum levels of interleukin-6 and 10 increases in COVID-19 infection, and the gene expression of these two interleukins underlying in this increase. The serum levels of IL-6, IL-10 and SARS-COV-2 IgG as well as CD4+ and CD8+ lymphocyte counts should be investigated to monitor patients and predict the course of disease.

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COVID-19 感染中白细胞介素-6 和白细胞介素-10 的基因调控
研究背景白细胞介素-6和10(IL6和IL-10)血清水平的作用和调节机制,以及CD4+和CD8+淋巴细胞与SARS-COV-2 IgM和IgG在COVID-19感染中的相互作用尚未完全清楚:本研究以 45 名 COVID-19 患者和 45 名健康人为对象。方法:本研究以 45 例 COVID-19 患者和 45 例健康人为研究对象,分别采用 qMSP-PCR、Real-time PCR、ELISA 和流式细胞术研究了 IL-6 和 IL-10 启动子甲基化、IL-6 和 IL-10 基因表达、SARS-COV-2 IgM 和 IgG 抗体以及 CD4+ 和 CD8+ 淋巴细胞:重症患者组的男性比例和平均年龄明显高于对照组(P< 0.05)。与对照组相比,患者的 IL-6 基因表达和血清水平均明显升高(P=0.002,0.001),但患者的 IL-6 启动子甲基化程度并未明显降低(P=0.835)。IL-10启动子甲基化和表达在病例和对照组之间无差异(0.326,0.455),但患者血清中的IL-10水平更高(P< 0.001)。与对照组相比,患者的CD4+和CD8+淋巴细胞减少(P< 0.001),平均SARS-COV-2 IgG增加(P=0.002):结论:COVID-19导致男性和老年人出现严重并发症。结论:COVID-19 会导致男性和老年人出现严重并发症,COVID-19 感染者血清中白细胞介素-6 和白细胞介素-10 的水平会升高,这两种白细胞介素的基因表达也是升高的基础。应检测血清中 IL-6、IL-10 和 SARS-COV-2 IgG 的水平以及 CD4+ 和 CD8+ 淋巴细胞计数,以监测患者病情并预测病程。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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