A novel donor-acceptor fluorescent probe for the fluorogenic/ chromogenic detection and bioimaging of nitric oxide

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL Analytica Chimica Acta Pub Date : 2024-02-05 DOI:10.1016/j.aca.2024.342333

Jingxin Hu , Ruiya Wang , Wantao Liao , Jinhui Hu , Lanqing Li , Zhen Cheng , Wen-Hua Chen

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Abstract

Nitric oxide (NO) plays an essential role in regulating various physiological and pathological processes. This has spurred various efforts to develop feasible methods for the detection of NO. Herein we designed and synthesized a novel donor-acceptor fluorescent probe Car-NO for the selective and specific detection of NO. Reaction of Car-NO with NO generated a new donor-acceptor structure with strong intramolecular charge transfer (ICT) effect, and led to remarkable chromogenic change from yellow to blue and dramatic fluorescence quenching. Car-NO exhibited high selectivity, excellent sensitivity, and rapid response for the detection of NO. In addition, the nanoparticles prepared from Car-NO (i.e., Car-NO NPs) showed strong NIR emission and high selectivity/sensitivity. Car-NO NPs was successfully employed to image both endogenous and exogenous NO in HeLa and RAW 264.7 cells. The present findings reveal that Car-NO is a promising probe for the detection and bioimaging of NO.

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用于一氧化氮的荧光/显色检测和生物成像的新型供体-受体荧光探针

一氧化氮（NO）在调节各种生理和病理过程中发挥着至关重要的作用。这促使人们努力开发检测一氧化氮的可行方法。在此，我们设计并合成了一种新型的供体-受体荧光探针 Car-NO，用于选择性和特异性地检测一氧化氮。Car-NO 与 NO 反应生成了一种新的供体-受体结构，具有很强的分子内电荷转移（ICT）效应，并导致显著的发色性变化，由黄色变为蓝色，荧光急剧淬灭。Car-NO 在检测 NO 方面具有高选择性、优异的灵敏度和快速反应能力。此外，由 Car-NO 制备的纳米颗粒（即 Car-NO NPs）显示出强烈的近红外发射和高选择性/灵敏度。Car-NO NPs 被成功用于对 HeLa 和 RAW 264.7 细胞中的内源性和外源性 NO 进行成像。目前的研究结果表明，Car-NO 是一种很有前景的检测和生物成像 NO 的探针。

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.