Pub Date : 2024-11-17DOI: 10.1016/j.aca.2024.343428
Shiwu Liu, Fangguo Lu, Shanquan Chen, Yi Ning
Background
Pathogenic bacteria are widespread in nature and can cause infections and various complications, thereby posing a severe risk to public health. Therefore, simple, rapid, sensitive, and cost-effective methods must be developed to detect pathogenic bacteria. Biosensors are prominent platforms for detecting pathogenic bacteria owing to their high sensitivity, specificity, repeatability, and stability. With the development of nanotechnology, graphene oxide (GO) has been increasingly introduced into the construction of fluorescent biosensors to enhance their performance owing to its unique physicochemical properties.
Results
This review systematically summarizes the development of GO-based fluorescent biosensors for the detection of pathogenic bacteria. First, we introduce the functionalization and modification of GO. The design and signal amplification strategies for GO-based fluorescent biosensors are also discussed. Finally, we explore the challenges and new perspectives associated with this field, with the aim of facilitating the development of GO-based fluorescent sensing technologies to prevent the spread of multidrug-resistant bacteria.
Significance
This review will aid in the development of high-performance biosensors for pathogenic bacterial assays.
背景致病菌在自然界中广泛存在，可引起感染和各种并发症，从而对公众健康构成严重威胁。因此，必须开发简单、快速、灵敏和经济有效的方法来检测致病细菌。生物传感器因其高灵敏度、特异性、可重复性和稳定性而成为检测致病细菌的重要平台。随着纳米技术的发展，氧化石墨烯（GO）因其独特的物理化学特性被越来越多地引入到荧光生物传感器的构建中，以提高其性能。首先，我们介绍了 GO 的功能化和修饰。还讨论了基于 GO 的荧光生物传感器的设计和信号放大策略。最后，我们探讨了与这一领域相关的挑战和新视角，旨在促进基于 GO 的荧光传感技术的发展，以防止多重耐药细菌的传播。
{"title":"Graphene oxide-based fluorescent biosensors for pathogenic bacteria detection: a review","authors":"Shiwu Liu, Fangguo Lu, Shanquan Chen, Yi Ning","doi":"10.1016/j.aca.2024.343428","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343428","url":null,"abstract":"<h3>Background</h3>Pathogenic bacteria are widespread in nature and can cause infections and various complications, thereby posing a severe risk to public health. Therefore, simple, rapid, sensitive, and cost-effective methods must be developed to detect pathogenic bacteria. Biosensors are prominent platforms for detecting pathogenic bacteria owing to their high sensitivity, specificity, repeatability, and stability. With the development of nanotechnology, graphene oxide (GO) has been increasingly introduced into the construction of fluorescent biosensors to enhance their performance owing to its unique physicochemical properties.<h3>Results</h3>This review systematically summarizes the development of GO-based fluorescent biosensors for the detection of pathogenic bacteria. First, we introduce the functionalization and modification of GO. The design and signal amplification strategies for GO-based fluorescent biosensors are also discussed. Finally, we explore the challenges and new perspectives associated with this field, with the aim of facilitating the development of GO-based fluorescent sensing technologies to prevent the spread of multidrug-resistant bacteria.<h3>Significance</h3>This review will aid in the development of high-performance biosensors for pathogenic bacterial assays.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"10 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-17DOI: 10.1016/j.aca.2024.343430
Hong Li, Yida Chen, Ze Fang, Yulan Lin, Lucio Frydman, Yu Yang, Zhong Chen
Background
Nuclear Magnetic Resonance (NMR) is extensively utilized in research as a non-invasive technique for investigating molecular structures and composite components. The spatiotemporal encoding (SPEN) technique effectively accelerates multi-dimensional NMR experiments. In ultrafast SPEN NMR, the acquired data are divided into odd and even segments corresponding to the positive and negative gradients during the decoding stage, respectively. However, the interlaced Fourier transform (FT) method used to reconstruct a full-width spectrum from these segments often suffers from severe noise contamination, necessitating the development of a more effective spectrum reconstruction method.
Results
In this work, we analyze the noise amplification effect of the interlaced FT and find that the noise is most significant in two edge regions of the spectrum along the indirect dimension due to the relatively small time offset differences between odd and even segments in those regions. Consequently, we develop an iterative optimization method to obtain the full-width spectrum while mitigating the noise. The proposed method incorporates the odd and even data segments into an objective function with sparsity regularization to simplify the spectrum, which is then refined iteratively during the optimization. As a result, the reconstructed spectrum is significantly cleaner and maintains the full spectral width. Experimental results demonstrate a remarkable improvement in the readability and interpretability of SPEN data, evidenced by clearer signal peaks and reduced background noise.
Significance
The proposed reconstruction method provides a reliable approach for processing SPEN 2D NMR data, effectively addressing the low sensitivity issue in the joint reconstruction on odd and even segments. Combining SPEN's ultrafast data acquisition with the proposed high-sensitivity spectrum reconstruction method enhances the utility of NMR for more accurate molecular structure analysis and component identification in composite samples, particularly promoting NMR research in rapid reaction systems.
{"title":"Enhanced spectral reconstruction of ultrafast spatiotemporal encoded 2D NMR spectroscopy","authors":"Hong Li, Yida Chen, Ze Fang, Yulan Lin, Lucio Frydman, Yu Yang, Zhong Chen","doi":"10.1016/j.aca.2024.343430","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343430","url":null,"abstract":"<h3>Background</h3>Nuclear Magnetic Resonance (NMR) is extensively utilized in research as a non-invasive technique for investigating molecular structures and composite components. The spatiotemporal encoding (SPEN) technique effectively accelerates multi-dimensional NMR experiments. In ultrafast SPEN NMR, the acquired data are divided into odd and even segments corresponding to the positive and negative gradients during the decoding stage, respectively. However, the interlaced Fourier transform (FT) method used to reconstruct a full-width spectrum from these segments often suffers from severe noise contamination, necessitating the development of a more effective spectrum reconstruction method.<h3>Results</h3>In this work, we analyze the noise amplification effect of the interlaced FT and find that the noise is most significant in two edge regions of the spectrum along the indirect dimension due to the relatively small time offset differences between odd and even segments in those regions. Consequently, we develop an iterative optimization method to obtain the full-width spectrum while mitigating the noise. The proposed method incorporates the odd and even data segments into an objective function with sparsity regularization to simplify the spectrum, which is then refined iteratively during the optimization. As a result, the reconstructed spectrum is significantly cleaner and maintains the full spectral width. Experimental results demonstrate a remarkable improvement in the readability and interpretability of SPEN data, evidenced by clearer signal peaks and reduced background noise.<h3>Significance</h3>The proposed reconstruction method provides a reliable approach for processing SPEN 2D NMR data, effectively addressing the low sensitivity issue in the joint reconstruction on odd and even segments. Combining SPEN's ultrafast data acquisition with the proposed high-sensitivity spectrum reconstruction method enhances the utility of NMR for more accurate molecular structure analysis and component identification in composite samples, particularly promoting NMR research in rapid reaction systems.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"9 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The overexpression of microRNA-222 (miRNA-222) is closely related to many human diseases, so the development of biosensors to detect this biomarker will contribute to the diagnosis of related diseases. Here, a simple, sensitive and specific fluorescence assay for the detection of miRNA-222 was developed using red-emitting upconversion nanoparticle (UCNP) as the donor and a DNA hairpin with black hole quencher-2 (BHQ-2) as the acceptor. Li+ and Tm3+-doped UCNP with a strong emission peak at 654 nm was obtained by changing the doped ion ratio and constructing core-shell structures. Under optimal conditions, the linear range for detecting miRNA-222 is 0.5∼2.5 nM and the limit of detection is as low as 0.077 nM without any complicated amplification strategy. Finally, the proposed assay was applied for the detection of miRNA-222 in serum samples. The results obtained were similar to those of the standard method, and the spiked recoveries were in the range of 97.62%-102.14%, suggesting that the proposed method has practical value in a complex biological sample matrix.
{"title":"Enhanced red emission of upconversion nanoparticles via Li+ and Tm3+ codoping and active core-shell construction for sensitive detection of miRNAs","authors":"Yingchao Li, Canzhao Tu, Qianshun Chen, Yingying Lin, Baoming Li, Haixia Lyu","doi":"10.1016/j.aca.2024.343429","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343429","url":null,"abstract":"The overexpression of microRNA-222 (miRNA-222) is closely related to many human diseases, so the development of biosensors to detect this biomarker will contribute to the diagnosis of related diseases. Here, a simple, sensitive and specific fluorescence assay for the detection of miRNA-222 was developed using red-emitting upconversion nanoparticle (UCNP) as the donor and a DNA hairpin with black hole quencher-2 (BHQ-2) as the acceptor. Li<sup>+</sup> and Tm<sup>3+</sup>-doped UCNP with a strong emission peak at 654 nm was obtained by changing the doped ion ratio and constructing core-shell structures. Under optimal conditions, the linear range for detecting miRNA-222 is 0.5∼2.5 nM and the limit of detection is as low as 0.077 nM without any complicated amplification strategy. Finally, the proposed assay was applied for the detection of miRNA-222 in serum samples. The results obtained were similar to those of the standard method, and the spiked recoveries were in the range of 97.62%-102.14%, suggesting that the proposed method has practical value in a complex biological sample matrix.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"41 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-14DOI: 10.1016/j.aca.2024.343425
Liguo Ji , Aoxiang Fu , Yuying Zhang, Ying Xu, Yanbei Xi, Shaoli Cui, Na Gao, Linlin Yang, Wanbing Shang, Zhijun Yang, Guangjie He
Background
Ischemia-reperfusion injury is a common cause of cardiovascular and cerebrovascular diseases. The reoxygenation during reperfusion leads to an overproduction of reactive oxygen species (ROS). As an antioxidant, H2S can scavenge ROS to inhibit oxidative stress and inflammatory reaction, thus attenuating ischemia-reperfusion injury. In this process, the changes of cellular microenvironment (polarity or viscosity) have not been fully discussed. In order to real-time track the changes of cellular microenvironment during the treatment of ischemia-reperfusion injury with H2S. It is necessary to develop highly selective and sensitive probes that can cascade response to hydrogen sulfide and cellular microenvironment.
Results
We designed and synthesized a fluorescent probe TPEC-DNBS which can produce cascade response to H2S and microenvironment. An intermediate TPEC-OH is produced after highly selective and sensitive response to H2S, which can further respond to polarity and viscosity. In addition, due to the aggregation-induced emission (AIE) and twisted intramolecular charge transfer (TICT) effects, polarity can promote the fluorescence emission wavelength and intensity of TPEC-OH to produce double response characteristics, and its change trend (from weak green fluorescence at low polarity to strong red fluorescence at high polarity) is opposite to that of traditional polar probes (from strong green fluorescence at low polarity to weak red fluorescence at high polarity). Viscosity can only induce the change of fluorescence intensity. By constructing the cardiomyocyte model and hepatocyte model of ischemia-reperfusion, we further prove that after ischemia-reperfusion injury, the cells are in an environment of low polarity, and the microenvironment can be recovered after H2S treatment.
Significance
An AIE-TICT fluorescence probe capable of cascading responses to H2S, polarity and viscosity was constructed by using tetraphenylethylene and coumarin moieties. This probe provides a more intuitive and convenient condition for real-time tracking the changes of cellular microenvironment (polarity or viscosity) before and after H2S treatment of ischemia-reperfusion injury.
{"title":"An AIE-TICT fluorescence probe cascade responsive to H2S, polarity and viscosity to track microenvironment changes in cellular model of ischemia-reperfusion injury","authors":"Liguo Ji , Aoxiang Fu , Yuying Zhang, Ying Xu, Yanbei Xi, Shaoli Cui, Na Gao, Linlin Yang, Wanbing Shang, Zhijun Yang, Guangjie He","doi":"10.1016/j.aca.2024.343425","DOIUrl":"10.1016/j.aca.2024.343425","url":null,"abstract":"<div><h3>Background</h3><div>Ischemia-reperfusion injury is a common cause of cardiovascular and cerebrovascular diseases. The reoxygenation during reperfusion leads to an overproduction of reactive oxygen species (ROS). As an antioxidant, H<sub>2</sub>S can scavenge ROS to inhibit oxidative stress and inflammatory reaction, thus attenuating ischemia-reperfusion injury. In this process, the changes of cellular microenvironment (polarity or viscosity) have not been fully discussed. In order to real-time track the changes of cellular microenvironment during the treatment of ischemia-reperfusion injury with H<sub>2</sub>S. It is necessary to develop highly selective and sensitive probes that can cascade response to hydrogen sulfide and cellular microenvironment.</div></div><div><h3>Results</h3><div>We designed and synthesized a fluorescent probe TPEC-DNBS which can produce cascade response to H<sub>2</sub>S and microenvironment. An intermediate TPEC-OH is produced after highly selective and sensitive response to H<sub>2</sub>S, which can further respond to polarity and viscosity. In addition, due to the aggregation-induced emission (AIE) and twisted intramolecular charge transfer (TICT) effects, polarity can promote the fluorescence emission wavelength and intensity of TPEC-OH to produce double response characteristics, and its change trend (from weak green fluorescence at low polarity to strong red fluorescence at high polarity) is opposite to that of traditional polar probes (from strong green fluorescence at low polarity to weak red fluorescence at high polarity). Viscosity can only induce the change of fluorescence intensity. By constructing the cardiomyocyte model and hepatocyte model of ischemia-reperfusion, we further prove that after ischemia-reperfusion injury, the cells are in an environment of low polarity, and the microenvironment can be recovered after H<sub>2</sub>S treatment.</div></div><div><h3>Significance</h3><div>An AIE-TICT fluorescence probe capable of cascading responses to H<sub>2</sub>S, polarity and viscosity was constructed by using tetraphenylethylene and coumarin moieties. This probe provides a more intuitive and convenient condition for real-time tracking the changes of cellular microenvironment (polarity or viscosity) before and after H<sub>2</sub>S treatment of ischemia-reperfusion injury.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343425"},"PeriodicalIF":5.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-14DOI: 10.1016/j.aca.2024.343427
Ziwei Zhang , Longyu Chen , Hongliang Wang , Bo Tang , Yongqiang Cheng , Meijia Zhu , Xiaotong Li , Xiaoxiao Qi , Yifan Shao , Xi Zhang
Background
Hydrogel microspheres with monodisperse and homogeneous dimensions have potential application in the field of three-dimensional (3D) cell culture due to its ability to provide a similar microenvironment. Currently, alginate hydrogel microspheres (AHMs) have received much attention due to the favorable properties of alginate such as biocompatibility, inexpensiveness, nontoxicity, and biodegradability. The fabrication methods of AHMs mainly include extrusion, electrostatic dripping and microfluidic chip techniques. These current methods suffer trade-offs between operational complexity, fabrication cost and practical application.
Results
We proposed a novel and versatile multi-well plate-based platform for online fabricating AHMs and in-situ 3D cell culture. The AHMs could be easily fabricated based on gravity-driven gelation combined with our recently developed bent-capillary-centrifugal-driven (BCCD) system. Ca-EDTA complex was used as Ca2+ source for crosslinking reaction of the alginate chains. The whole preparation process of AHMs included four steps: emulsification, pre-gelation, spontaneous demulsification and further solidification. The gravity-driven hydrogel microsphere gelation could produce the AHMs with good sphericity (Φ = 0.96) and monodispersity (PDI% = 0.94 %). The rapid drug susceptibility testing and single-cell encapsulation in the AHMs were well demonstrated. It also provided a novel in-situ 3D cell culture strategy, which demonstrated more than 85 % cell viability in practice.
Significance
The proposed platform avoided the complex and laborious microfabrication. Moreover, cell-encapsulated AHMs could be directly produced in the multi-well plate, which could facilitate the subsequent cultivation and observation. It is expected to be a versatile in-situ 3D cell culture tool in the fields of biomedicine and tissue engineering.
{"title":"Multi-well plate-based versatile platform for online fabricating alginate hydrogel microspheres and in-situ 3D cell culture","authors":"Ziwei Zhang , Longyu Chen , Hongliang Wang , Bo Tang , Yongqiang Cheng , Meijia Zhu , Xiaotong Li , Xiaoxiao Qi , Yifan Shao , Xi Zhang","doi":"10.1016/j.aca.2024.343427","DOIUrl":"10.1016/j.aca.2024.343427","url":null,"abstract":"<div><h3>Background</h3><div>Hydrogel microspheres with monodisperse and homogeneous dimensions have potential application in the field of three-dimensional (3D) cell culture due to its ability to provide a similar microenvironment. Currently, alginate hydrogel microspheres (AHMs) have received much attention due to the favorable properties of alginate such as biocompatibility, inexpensiveness, nontoxicity, and biodegradability. The fabrication methods of AHMs mainly include extrusion, electrostatic dripping and microfluidic chip techniques. These current methods suffer trade-offs between operational complexity, fabrication cost and practical application.</div></div><div><h3>Results</h3><div>We proposed a novel and versatile multi-well plate-based platform for online fabricating AHMs and in-situ 3D cell culture. The AHMs could be easily fabricated based on gravity-driven gelation combined with our recently developed bent-capillary-centrifugal-driven (BCCD) system. Ca-EDTA complex was used as Ca<sup>2+</sup> source for crosslinking reaction of the alginate chains. The whole preparation process of AHMs included four steps: emulsification, pre-gelation, spontaneous demulsification and further solidification. The gravity-driven hydrogel microsphere gelation could produce the AHMs with good sphericity (Φ = 0.96) and monodispersity (PDI% = 0.94 %). The rapid drug susceptibility testing and single-cell encapsulation in the AHMs were well demonstrated. It also provided a novel in-situ 3D cell culture strategy, which demonstrated more than 85 % cell viability in practice.</div></div><div><h3>Significance</h3><div>The proposed platform avoided the complex and laborious microfabrication. Moreover, cell-encapsulated AHMs could be directly produced in the multi-well plate, which could facilitate the subsequent cultivation and observation. It is expected to be a versatile in-situ 3D cell culture tool in the fields of biomedicine and tissue engineering.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343427"},"PeriodicalIF":5.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-14DOI: 10.1016/j.aca.2024.343426
Ya Zhou , Li Yang , Xuemei Zhang , Li Zhu , Xiaoli Xiong , Ting Xiao , Liping Zhu
Background
Pesticide residues can cause chronic toxicity to the human body and lead to a series of diseases that damage the liver. Therefore, developing a highly sensitive, selective, and low-cost pesticide residues detection method is of great significance for protecting human health and safety. Nowadays, commonly used methods for pesticide residue detection include gas chromatography, high-performance liquid chromatography, and fluorescence sensing. These methods have some typical shortcomings, such as long sample pretreatment time, expensive instruments, and poor controllability. It was thought that a sensing platform based on electrochemical analysis method and functional DNA molecules can eliminate the above drawbacks.
Results
Herein, this study developed a simple and label-free electrochemical aptasensor based on a triple-stranded DNA molecular switch. Acetamiprid (ACE) was served as the analytical model, and its binding with the aptamer opened the triple-stranded DNA molecular switch, resulting in the in-situ formation of G-quadruplex/hemin complexes on the electrode surface, obtaining a significantly enhanced electrochemical signal and achieving high specificity and label-free detection of ACE, with a detection limit as low as 4.67 × 10−3 nM (S/N = 3). In addition, due to the specific recognition between the aptamer and the target, the aptasensor effectively avoided the interference of other pesticides and exhibited good specificity. Moreover, benefiting from the pH-switchable of the triple-stranded DNA molecular switch and the programmability of DNA molecules, “OR” logic gate and “OR-INHIBIT” cascade logic circuit were successfully implemented.
Significance
The proposed electrochemical aptasensor exhibited good accuracy and sensitivity in detecting acetamiprid in vegetable soil sample, indicating its practicality in the detection of pesticide residues in actual samples. Furthermore, the sensing system was reasonably programmed and successfully operated an “OR” logic gate and an “OR-INHIBIT” cascade logic circuit, demonstrating its potential application in intelligent sensing.
背景农药残留会对人体产生慢性毒性，导致一系列损害肝脏的疾病。因此，开发一种高灵敏度、高选择性、低成本的农药残留检测方法对保护人类健康和安全具有重要意义。目前，常用的农药残留检测方法包括气相色谱法、高效液相色谱法和荧光传感法。这些方法都存在一些典型的缺点，如样品前处理时间长、仪器昂贵、可控性差等。本研究开发了一种基于三链 DNA 分子开关的简单、无标记的电化学传感器。以啶虫脒（ACE）为分析模型，其与aptamer的结合打开了三链DNA分子开关，从而在电极表面原位形成了G-四链/hemin复合物，获得了显著增强的电化学信号，实现了对ACE的高特异性和无标记检测，检测限低至4.67×10-3 nM（S/N=3）。此外，由于适配体与目标物之间的特异性识别，该适配传感器有效地避免了其他农药的干扰，表现出良好的特异性。此外，利用三链 DNA 分子开关的 pH 值可调和 DNA 分子的可编程性，成功实现了 "OR "逻辑门和 "OR-INHIBIT "级联逻辑电路。此外，该传感系统经过合理编程，成功运行了 "OR "逻辑门和 "OR-INHIBIT "级联逻辑电路，证明了其在智能传感方面的应用潜力。
{"title":"Construction of label-free electrochemical aptasensor and logic circuit based on triple-stranded DNA molecular switch","authors":"Ya Zhou , Li Yang , Xuemei Zhang , Li Zhu , Xiaoli Xiong , Ting Xiao , Liping Zhu","doi":"10.1016/j.aca.2024.343426","DOIUrl":"10.1016/j.aca.2024.343426","url":null,"abstract":"<div><h3>Background</h3><div>Pesticide residues can cause chronic toxicity to the human body and lead to a series of diseases that damage the liver. Therefore, developing a highly sensitive, selective, and low-cost pesticide residues detection method is of great significance for protecting human health and safety. Nowadays, commonly used methods for pesticide residue detection include gas chromatography, high-performance liquid chromatography, and fluorescence sensing. These methods have some typical shortcomings, such as long sample pretreatment time, expensive instruments, and poor controllability. It was thought that a sensing platform based on electrochemical analysis method and functional DNA molecules can eliminate the above drawbacks.</div></div><div><h3>Results</h3><div>Herein, this study developed a simple and label-free electrochemical aptasensor based on a triple-stranded DNA molecular switch. Acetamiprid (ACE) was served as the analytical model, and its binding with the aptamer opened the triple-stranded DNA molecular switch, resulting in the in-situ formation of G-quadruplex/hemin complexes on the electrode surface, obtaining a significantly enhanced electrochemical signal and achieving high specificity and label-free detection of ACE, with a detection limit as low as 4.67 × 10<sup>−3</sup> nM (S/N = 3). In addition, due to the specific recognition between the aptamer and the target, the aptasensor effectively avoided the interference of other pesticides and exhibited good specificity. Moreover, benefiting from the pH-switchable of the triple-stranded DNA molecular switch and the programmability of DNA molecules, “OR” logic gate and “OR-INHIBIT” cascade logic circuit were successfully implemented.</div></div><div><h3>Significance</h3><div>The proposed electrochemical aptasensor exhibited good accuracy and sensitivity in detecting acetamiprid in vegetable soil sample, indicating its practicality in the detection of pesticide residues in actual samples. Furthermore, the sensing system was reasonably programmed and successfully operated an “OR” logic gate and an “OR-INHIBIT” cascade logic circuit, demonstrating its potential application in intelligent sensing.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343426"},"PeriodicalIF":5.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-13DOI: 10.1016/j.aca.2024.343414
Qi Wu , Yuan Chen , Yan-Li Wang , Ji-Ying Song , Hai-Tao Lv , Ya-Ming Sun
Background
Chirality is an essential property of nature. Chiral recognition is of great significance to life sciences, pharmaceutical industry, food analysis, and so on. Chiral carbon dots (CCDs), as green nanomaterials, have great prospects in chiral sensing. However, CCDs with enantioselectivity for tryptophan (Trp) enantiomers are scarce. Moreover, most chiral sensing platforms depend on the difference of fluorescence intensity at the same emission wavelength to identify enantiomers, it is still a challenge to distinguish enantiomers by the positions of fluorescent emission peaks.
Results
Novel CCDs with specific chiral recognition ability for Trp enantiomers are synthesized using l-lysine and l-cysteine as precursors. The CCDs have two fluorescent emission peaks at 390 nm and 450 nm. Interestingly, the fluorescence intensity of CCDs at 390 nm enhances obviously on the addition of L-Trp, while it enhances slightly at 450 nm in the presence of D-Trp. This chiral sensing system not only can identify Trp enantiomers according to fluorescence intensity, but also achieves the distinguishment depending on emission wavelength. The enantioselectivity (IL/ID) reaches 4.5 when the concentration of Trp enantiomer is 1 mM. This chiral sensing platform not only can be used for quantitative analysis of D-Trp and L-Trp, but also can be used for determining the enantiomeric excess of racemates. The chiral recognition mechanism is investigated by molecular simulation. It is found that L-Trp has higher binding energy with CCDs.
Significance
This work presents a novel kind of CCDs with special chiral recognition performance for Trp enantiomers, and opens the door to identify chiral isomers according to wavelength difference, which has profound significance for the development of chiral sensing platforms, and may provide inspirations for the design of novel CCDs with excellent chiral recognition performance.
{"title":"Dual emission chiral carbon dots as fluorescent probe for fast chiral recognition of tryptophan enantiomers","authors":"Qi Wu , Yuan Chen , Yan-Li Wang , Ji-Ying Song , Hai-Tao Lv , Ya-Ming Sun","doi":"10.1016/j.aca.2024.343414","DOIUrl":"10.1016/j.aca.2024.343414","url":null,"abstract":"<div><h3>Background</h3><div>Chirality is an essential property of nature. Chiral recognition is of great significance to life sciences, pharmaceutical industry, food analysis, and so on. Chiral carbon dots (CCDs), as green nanomaterials, have great prospects in chiral sensing. However, CCDs with enantioselectivity for tryptophan (Trp) enantiomers are scarce. Moreover, most chiral sensing platforms depend on the difference of fluorescence intensity at the same emission wavelength to identify enantiomers, it is still a challenge to distinguish enantiomers by the positions of fluorescent emission peaks.</div></div><div><h3>Results</h3><div>Novel CCDs with specific chiral recognition ability for Trp enantiomers are synthesized using <span>l</span>-lysine and <span>l</span>-cysteine as precursors. The CCDs have two fluorescent emission peaks at 390 nm and 450 nm. Interestingly, the fluorescence intensity of CCDs at 390 nm enhances obviously on the addition of L-Trp, while it enhances slightly at 450 nm in the presence of D-Trp. This chiral sensing system not only can identify Trp enantiomers according to fluorescence intensity, but also achieves the distinguishment depending on emission wavelength. The enantioselectivity (I<sub>L</sub>/I<sub>D</sub>) reaches 4.5 when the concentration of Trp enantiomer is 1 mM. This chiral sensing platform not only can be used for quantitative analysis of D-Trp and L-Trp, but also can be used for determining the enantiomeric excess of racemates. The chiral recognition mechanism is investigated by molecular simulation. It is found that L-Trp has higher binding energy with CCDs.</div></div><div><h3>Significance</h3><div>This work presents a novel kind of CCDs with special chiral recognition performance for Trp enantiomers, and opens the door to identify chiral isomers according to wavelength difference, which has profound significance for the development of chiral sensing platforms, and may provide inspirations for the design of novel CCDs with excellent chiral recognition performance.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343414"},"PeriodicalIF":5.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-13DOI: 10.1016/j.aca.2024.343415
Jiaqi Liao , Chonghao Li , Jiajia Liu , Hao Cheng , Linmin Li , Guixi Tang , Ru Huang , Yating Lu , Siyue Chen , Qian Zhang , Hao Chen , Qinyuan Chen , Hong Chen , Daoheng Sun
The detection of nucleic acids, which typically consists of nucleic acid extraction, reverse transcription and amplification, is a crucial component of molecular diagnostics. However, traditional methods have several limitations, including high reliance on skilled personnel, low degree of automation and lengthy assay times. Additionally, these methods also require separate areas for the different steps to avoid contamination. Though a number of digital microfluidic (DMF) chips have been reported to detect the nucleic acids, but there is a noticeable absence of fully integrated DMF platforms capable of concurrently performing nucleic acid extraction, amplification, and signal detection. Here, a DMF chip combined with a dual-mode thermal control module was developed to realize the automatic and integrated detection of nucleic acids. The dual-mode thermal control module provided the required temperatures (with a range from 4 °C to 95 °C) for all the steps of nucleic acid detection. Pseudoviruses of SARS-CoV-2 and monkeypox were chosen to demonstrate the feasibility of the DMF chip, and all assays were completed within 40 min. The results confirmed that the products extracted under 4 °C exhibited high concentration and integrity, which were comparable to those by traditional methods. And the analytical performance of amplify was comparable to that of traditional methods with a more compact design, shorter time, smaller size and more automatic operation. With the ability of dual-mode thermo control and full automatic operation, nucleic acid detection with the characteristics of sample-in-result-out was realized on the DMF chip, which holds great promise in fields such as in vitro diagnostics and point‐of‐care testing.
{"title":"Automatic and integrated detection of nucleic acid by using a dual-mode thermal controlled digital microfluidic chip","authors":"Jiaqi Liao , Chonghao Li , Jiajia Liu , Hao Cheng , Linmin Li , Guixi Tang , Ru Huang , Yating Lu , Siyue Chen , Qian Zhang , Hao Chen , Qinyuan Chen , Hong Chen , Daoheng Sun","doi":"10.1016/j.aca.2024.343415","DOIUrl":"10.1016/j.aca.2024.343415","url":null,"abstract":"<div><div>The detection of nucleic acids, which typically consists of nucleic acid extraction, reverse transcription and amplification, is a crucial component of molecular diagnostics. However, traditional methods have several limitations, including high reliance on skilled personnel, low degree of automation and lengthy assay times. Additionally, these methods also require separate areas for the different steps to avoid contamination. Though a number of digital microfluidic (DMF) chips have been reported to detect the nucleic acids, but there is a noticeable absence of fully integrated DMF platforms capable of concurrently performing nucleic acid extraction, amplification, and signal detection. Here, a DMF chip combined with a dual-mode thermal control module was developed to realize the automatic and integrated detection of nucleic acids. The dual-mode thermal control module provided the required temperatures (with a range from 4 °C to 95 °C) for all the steps of nucleic acid detection. Pseudoviruses of SARS-CoV-2 and monkeypox were chosen to demonstrate the feasibility of the DMF chip, and all assays were completed within 40 min. The results confirmed that the products extracted under 4 °C exhibited high concentration and integrity, which were comparable to those by traditional methods. And the analytical performance of amplify was comparable to that of traditional methods with a more compact design, shorter time, smaller size and more automatic operation. With the ability of dual-mode thermo control and full automatic operation, nucleic acid detection with the characteristics of sample-in-result-out was realized on the DMF chip, which holds great promise in fields such as in vitro diagnostics and point‐of‐care testing.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343415"},"PeriodicalIF":5.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-13DOI: 10.1016/j.aca.2024.343418
Guobin Mao , Yuan Zeng , Chunmin Qiu , Guangmiao Ding , Leyao Li , Lixin Ma , Junbiao Dai , Wen Yin , Yingxin Ma
Accurately monitoring the freshness of high-protein foods has significant implications for both food safety and public welfare. Since a large amount of hydrogen sulfide (H2S) is produced during spoilage-related processes, abnormal H2S levels are often considered an important indicator of food spoilage. Therefore, we synthesized novel nanoparticles (NPs) containing Silicon (Si) dots and CdTe quantum dots to accurately assess the amount of sulfide ions (S2−) and thus the quality of high-protein foods in the early stage of storage. As the concentration of S2− increased, the fluorescence intensity of Si/CdTe NPs at λem = 488 nm increased, while the fluorescence intensity at λem = 620 nm was quenched. The fluorescence intensity ratio (F620/F488) was negatively linearly correlated to S2− concentrations in the range of 1–20 μM, with a detection limit of 0.3 μM. Furthermore, to achieve portable detection, we mixed Si/CdTe NPs with sodium carboxymethyl cellulose to prepare effective visual fluorescent sensing paper chips, which exhibited ideal porous structure, good particle dispersion, and excellent fluorescence properties. Incubating the paper chips with high-protein foods allowed for accurate monitoring of food freshness during storage. Therefore, this approach provided a reliable and portable method to determine H2S concentration using a novel concept to ensure the freshness and safety of high-protein foods.
{"title":"Ratiometric fluorescent paper chip for monitoring the freshness of high protein foods","authors":"Guobin Mao , Yuan Zeng , Chunmin Qiu , Guangmiao Ding , Leyao Li , Lixin Ma , Junbiao Dai , Wen Yin , Yingxin Ma","doi":"10.1016/j.aca.2024.343418","DOIUrl":"10.1016/j.aca.2024.343418","url":null,"abstract":"<div><div>Accurately monitoring the freshness of high-protein foods has significant implications for both food safety and public welfare. Since a large amount of hydrogen sulfide (H<sub>2</sub>S) is produced during spoilage-related processes, abnormal H<sub>2</sub>S levels are often considered an important indicator of food spoilage. Therefore, we synthesized novel nanoparticles (NPs) containing Silicon (Si) dots and CdTe quantum dots to accurately assess the amount of sulfide ions (S<sup>2−</sup>) and thus the quality of high-protein foods in the early stage of storage. As the concentration of S<sup>2−</sup> increased, the fluorescence intensity of Si/CdTe NPs at λ<sub>em</sub> = 488 nm increased, while the fluorescence intensity at λ<sub>em</sub> = 620 nm was quenched. The fluorescence intensity ratio (F<sub>620</sub>/F<sub>488</sub>) was negatively linearly correlated to S<sup>2−</sup> concentrations in the range of 1–20 μM, with a detection limit of 0.3 μM. Furthermore, to achieve portable detection, we mixed Si/CdTe NPs with sodium carboxymethyl cellulose to prepare effective visual fluorescent sensing paper chips, which exhibited ideal porous structure, good particle dispersion, and excellent fluorescence properties. Incubating the paper chips with high-protein foods allowed for accurate monitoring of food freshness during storage. Therefore, this approach provided a reliable and portable method to determine H<sub>2</sub>S concentration using a novel concept to ensure the freshness and safety of high-protein foods.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343418"},"PeriodicalIF":5.7,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aminoglycoside antibiotics (AGs) are commonly utilized in both human and veterinary medicine to treat and manage a range of infections. These antibiotics are recognized for their narrow therapeutic window, with an overdose potentially resulting in severe side effects like kidney and ear damage. Hence, the implementation of a quick, precise, and on-the-spot testing method is crucial in clinical settings. In the present investigation, we designed an innovative indirect lateral flow assay (LFA) utilizing aptamers to detect kanamycin, a type of aminoglycoside antibiotics. We prepared mesoporous silica nanoparticles (MSNs) functionalized with amino groups, loaded them with morphine (MOP), and then sealed them with aminoglycoside aptamers (Apt). The complex of gated-MSNs@Apt was tested using the MOP LFA in the presence or absence of kanamycin antibiotics.
Results
The indirect LFA system displayed a single colored line in the test line for positive samples, whereas it exhibited double colored lines in both the control line and test line for negative samples. Our custom-designed LFA biosensors demonstrated two linear ranges, from 10 nM to 350 nM and 500 nM–1500 nM, with a limit of detection (LOD) of 5 nM in serum media under optimized conditions. The introduced indirect LFA biosensor was effectively utilized to detect kanamycin, achieving a satisfactory recovery rate of 92.9–109.9 % with RSD of 1.68–7.73 % in serum samples.
Significance
In general, the created LFA system offers a portable, straightforward, and affordable approach for point-of-care (POC) identification of kanamycin and other AGs.
{"title":"An innovative biosensor utilizing aptamer-gated mesoporous silica nanoparticles for determination of aminoglycoside antibiotics through indirect-lateral flow","authors":"Reza Ghaffari , Reza Moradi , NoorMohammad Danesh , Mohammad Ramezani , Mona Alibolandi , Khalil Abnous , Seyed Mohammad Taghdisi","doi":"10.1016/j.aca.2024.343413","DOIUrl":"10.1016/j.aca.2024.343413","url":null,"abstract":"<div><h3>Background</h3><div>Aminoglycoside antibiotics (AGs) are commonly utilized in both human and veterinary medicine to treat and manage a range of infections. These antibiotics are recognized for their narrow therapeutic window, with an overdose potentially resulting in severe side effects like kidney and ear damage. Hence, the implementation of a quick, precise, and on-the-spot testing method is crucial in clinical settings. In the present investigation, we designed an innovative indirect lateral flow assay (LFA) utilizing aptamers to detect kanamycin, a type of aminoglycoside antibiotics. We prepared mesoporous silica nanoparticles (MSNs) functionalized with amino groups, loaded them with morphine (MOP), and then sealed them with aminoglycoside aptamers (Apt). The complex of gated-MSNs@Apt was tested using the MOP LFA in the presence or absence of kanamycin antibiotics.</div></div><div><h3>Results</h3><div>The indirect LFA system displayed a single colored line in the test line for positive samples, whereas it exhibited double colored lines in both the control line and test line for negative samples. Our custom-designed LFA biosensors demonstrated two linear ranges, from 10 nM to 350 nM and 500 nM–1500 nM, with a limit of detection (LOD) of 5 nM in serum media under optimized conditions. The introduced indirect LFA biosensor was effectively utilized to detect kanamycin, achieving a satisfactory recovery rate of 92.9–109.9 % with RSD of 1.68–7.73 % in serum samples.</div></div><div><h3>Significance</h3><div>In general, the created LFA system offers a portable, straightforward, and affordable approach for point-of-care (POC) identification of kanamycin and other AGs.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1334 ","pages":"Article 343413"},"PeriodicalIF":5.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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