The effect of oscillation depolymerization on ethylenediaminetetraacetic acid-dependent platelet aggregation samples: A cross-over study

IF 2.2 4区医学 Q3 HEMATOLOGY International Journal of Laboratory Hematology Pub Date : 2024-02-07 DOI:10.1111/ijlh.14246

Dan Liu, Wei Yang

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Abstract

Introduction

Ethylenediaminetetraacetic acid (EDTA)-dependent platelet aggregation (PA) can cause medical errors. Currently, there is no reliable method for completely solving this problem. This study aims to solve this problem that has plagued clinical practice for many years by using oscillation method.

Methods

Sixty-one EDTA-PA samples were collected, divided, and disaggregated using the oscillation method at various times and speeds. The samples were analyzed using routine blood tests and blood smears.

Results

Platelet counts (PLT) were increased significantly after oscillation. PLT in the 3000 rpm for 0.5 min group was significantly higher than that in the 500 rpm for 0.5 min group (p < 0. 01). After 3000 rpm oscillation, the PLT gradually increased with time, while compared with the 10-min group, the PLT in the 13-min group showed no significant differences. The effective disaggregation rates in the EDTA-PA samples using the oscillation method and sodium citrate anticoagulant were 96.72% and 65.57%, respectively. There were no significant changes in white blood cell (WBC) or red blood cell (RBC) counts or morphology after the use of the oscillation method.

Conclusion

The oscillation method effectively depolymerized EDTA-PA without adverse effects on WBC and RBC. The implementation of this technique promises to resolve the issue of EDTA-PA.

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振荡解聚对乙二胺四乙酸依赖性血小板聚集样本的影响：交叉研究

导言：乙二胺四乙酸（EDTA）依赖性血小板聚集（PA）可导致医疗事故。目前，还没有一种可靠的方法能彻底解决这一问题。本研究旨在利用振荡法解决这个困扰临床多年的问题：方法：采用不同时间和速度的振荡法收集、分割和分解 61 份 EDTA-PA 样品。结果：血小板计数（PLT）和血细胞比容（PLT）均高于正常值：结果：振荡后血小板计数（PLT）明显增加。3000 转/分钟 0.5 分钟组的 PLT 明显高于 500 转/分钟 0.5 分钟组（p 结论：振荡法能有效地解聚血小板：振荡法能有效解聚 EDTA-PA，对白细胞和红细胞无不良影响。该技术的应用有望解决 EDTA-PA 的问题。

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来源期刊

International Journal of Laboratory Hematology 医学-血液学

CiteScore

4.50

自引率

6.70%

发文量

211

审稿时长

6-12 weeks

期刊介绍： The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology. The journal publishes invited reviews, full length original articles, and correspondence. The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines. The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.