International Journal of Laboratory Hematology最新文献

{"title":"Comparison of Three ELISA Assays for the Detection and Quantification of Autoantibodies Against Complement Factor H","authors":"Sandrine Thouzeau-Benghezal, Clémence Merlen, Emmanuelle Pépin, Laurine Boulanger, Rayan Yousfi, Anne-Laure Lapeyraque, Alexandra Cambier, Georges-Etienne Rivard, Stéphan Troyanov, Arnaud Bonnefoy","doi":"10.1111/ijlh.70028","DOIUrl":"10.1111/ijlh.70028","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"48 1","pages":"227-231"},"PeriodicalIF":2.3,"publicationDate":"2025-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ICSH Recommendations for Monocyte Cell Lineage Morphologic Identification, Nomenclature Harmonization, and Utilization as a Biomarker","authors":"Gina Zini,&nbsp;Yoon Hwan Chang,&nbsp;Giuseppe d'Onofrio,&nbsp;John Frater,&nbsp;Ulrich Germing,&nbsp;Anna Merino,&nbsp;Olga Pozdnyakova,&nbsp;David Ross,&nbsp;Claudio Romulo Siqueira Filho,&nbsp;Akiyoshi Takami,&nbsp;Erber Wendy","doi":"10.1111/ijlh.70029","DOIUrl":"10.1111/ijlh.70029","url":null,"abstract":"<p>Monocytes are key components of the Mononuclear Phagocyte System, crucial in immune defense, inflammation, and tissue repair. Accurate identification and classification of monocyte lineage cells are essential for diagnosing both reactive and clonal hematologic disorders. However, morphological criteria and nomenclature inconsistencies have hindered reproducibility, particularly with the rise of automated and AI-driven diagnostic tools. The International Council for Standardization in Haematology (ICSH) Monocyte Working Group (MWG) was convened to establish standardized morphological definitions, harmonize nomenclature for monocytes and their precursors, and evaluate their clinical utility as biomarkers. Data were collected from global laboratories, combined with an extensive literature review and consensus feedback from the ICSH General Assembly. The MWG proposes a three-category morphological classification: blasts and blast equivalents (monoblasts and promonocytes), immature monocytes, and mature monocytes. The recommendations reaffirm the importance of morphological analysis, cytochemical staining, and flow cytometry for accurate diagnosis. Emerging automated parameters, such as monocyte distribution width (MDW), and ratios like lymphocyte/monocyte (LMR) and neutrophil/monocyte (NMR), are also recognized as valuable adjunctive biomarkers. Cytogenetic and molecular results may also impact the utilization of monocytes as a biomarker. These harmonized recommendations aim to improve diagnostic accuracy, support the development of machine learning tools, and facilitate consistent reporting across laboratories worldwide.</p>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"48 1","pages":"12-25"},"PeriodicalIF":2.3,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reevaluating Lipemic Interference in D-Dimer Measurement: Evidence of Instrument-Dependent Overestimation","authors":"Jia Du,&nbsp;Xiaohui Liu,&nbsp;Zhenlu Zhang,&nbsp;Litao Zhang","doi":"10.1111/ijlh.70022","DOIUrl":"10.1111/ijlh.70022","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"48 1","pages":"223-226"},"PeriodicalIF":2.3,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145491005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-Transplant Aggressive NK-Cell Leukemia: De Novo Versus Lineage-Shift With Recurrent Hepatosplenic T-Cell Lymphoma in the Setting of Chronic EBV Viremia","authors":"Juanyu Ke,&nbsp;Yiwen Zhou,&nbsp;Lei Chen,&nbsp;Siping Chen,&nbsp;Xi Lan","doi":"10.1111/ijlh.70016","DOIUrl":"10.1111/ijlh.70016","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"48 1","pages":"3-4"},"PeriodicalIF":2.3,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145454352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishing Reference Ranges and Evaluating Clinical Factors of the Complete Blood Count in Neonates Admitted to a Neonatal Intensive Care Unit","authors":"Emily Hyde,&nbsp;Jarob Saker,&nbsp;Stephen Kennedy,&nbsp;Mark Anthony,&nbsp;Manu Vatish","doi":"10.1111/ijlh.70014","DOIUrl":"10.1111/ijlh.70014","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Accurate complete blood count (CBC) reference intervals are essential for neonatal care. However, existing reference intervals do not account for key clinical variables such as sex, postnatal age, gestational age at birth and corticosteroid exposure. This study aims to establish updated CBC reference intervals for neonates admitted to a Neonatal Intensive Care Unit (NICU) while evaluating the effects of these factors on the CBC.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In this retrospective cohort study, all neonates admitted to the NICU at the John Radcliffe Hospital (Oxford, United Kingdom) between January 2022 and January 2023 were eligible for inclusion. Routine CBCs were included if there was no suspicion of infection or necrotising enterocolitis, no recent surgical interventions and no signs of clinical deterioration. The effects of sex, gestational age at birth and postnatal age were assessed for 48 parameters of the CBC using multivariate ANOVAs. Reference intervals were calculated at the 95% level.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among 3490 CBC results from 587 neonates, 386 results from 196 neonates met inclusion criteria. Sex-related differences were observed in nine parameters. Gestational and postnatal age both significantly influenced 34 parameters. Reference intervals were produced for all 48 CBC parameters, with histograms and boxplots illustrating variations by sex, postnatal age and gestational age. Secondary analyses highlighted the effects of corticosteroid exposure.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We present reference intervals for 48 neonatal CBC parameters, highlighting the influence of sex, postnatal age, gestational age at birth and corticosteroid exposure. These findings improve the interpretation of neonatal CBCs and propose criteria for defining a sufficiently healthy neonatal population for diagnostic research.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"48 1","pages":"81-92"},"PeriodicalIF":2.3,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.70014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145460985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complex t(8;14;18)(q24;q32;q21) Together With Novel t(3;6)(q27;p23) in Triple-Hit High-Grade B-Cell Lymphoma Showing Burkitt-Like Cytology","authors":"Katsuya Yamamoto,&nbsp;Keiji Kurata,&nbsp;Kimikazu Yakushijin,&nbsp;Hironobu Minami","doi":"10.1111/ijlh.70018","DOIUrl":"10.1111/ijlh.70018","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"48 1","pages":"5-8"},"PeriodicalIF":2.3,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145357421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artefactual Heat Induced Full Blood Examination Changes in a Patient With Cold Agglutinins","authors":"Benjamin L. Hartog,&nbsp;G. Rowley,&nbsp;S. Juneja","doi":"10.1111/ijlh.70020","DOIUrl":"10.1111/ijlh.70020","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"48 1","pages":"9-11"},"PeriodicalIF":2.3,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145357323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Clinical Significance of CD371 Expression Detected by Flow Cytometry in Primary Myelofibrosis","authors":"Zhang Bingyao,&nbsp;Zhang Xuxi,&nbsp;Fu Zhaoqiang,&nbsp;Yang Qian,&nbsp;Qin Youwen","doi":"10.1111/ijlh.70015","DOIUrl":"10.1111/ijlh.70015","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aims to analyze the expression patterns of CD371 in CD34⁺CD117⁺ bone marrow (BM) cells from patients with primary myelofibrosis (PMF) using flow cytometry (FCM), with a comparative evaluation against conventional antigens to assess its potential clinical utility for identifying aberrant immunophenotypes in PMF.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A retrospective analysis was conducted on BM samples from 26 PMF patients and 20 control individuals. We evaluated the proportions of CD34⁺CD117⁺ cells and basophils, as well as the expression profiles—including positive cell percentages, mean fluorescence intensity (MFI), and coefficient of variation (CV) of MFI—of CD371, CD34, CD117, CD13, CD33, CD123, CD38, HLA-DR, and CD7 within the CD34⁺CD117⁺ population.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Control group exhibited consistent bimodal CD371 expression, while PMF samples showed three distinct patterns. PMF demonstrated significant differences vs. controls in CD371 MFI (<i>p</i> &lt; 0.01), MFI CV (<i>p</i> &lt; 0.01), and CD371⁺ cell proportion (<i>p</i> &lt; 0.05). Within CD371⁺ cells, MFI and MFI CV also differed significantly (both <i>p</i> &lt; 0.01). Significant differences (<i>p</i> &lt; 0.01) were observed in: CD34⁺CD117⁺ proportion, CD34 MFI/MFI CV, basophil proportion, CD38^dim+ proportion/CD38 MFI/MFI CV. No significant differences were observed for CD13⁺ cell proportion (<i>p</i> = 0.154) or CD13 MFI (<i>p</i> = 0.835), though the MFI CV was significantly different (<i>p</i> &lt; 0.01). CD33⁺ cell proportion (<i>p</i> &lt; 0.05) and CD33 MFI (<i>p</i> &lt; 0.05) showed significant differences, while the MFI CV did not (<i>p</i> = 0.276).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study provides the first characterization of CD371 expression patterns in PMF, showing significantly different expression profiles compared to controls. While CD371 demonstrated comparable performance to standard markers (CD34/CD38/CD13/CD33) and showed better discrimination than CD123/HLA-DR, these preliminary findings suggest its potential utility for: (1) identifying abnormal CD34⁺CD117⁺ populations in PMF, and (2) possible integration into routine clinical workflows, pending further validation in larger cohorts.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"48 1","pages":"110-116"},"PeriodicalIF":2.3,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145294805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pediatric Severe Eosinophilia: Etiological Spectrum, Diagnostic Algorithm, and Case-Based Insights From a Tertiary Care Center","authors":"Neha Goel,&nbsp;Sumit Mehndiratta,&nbsp;Satyendra Batra,&nbsp;Amitabh Singh,&nbsp;Prashant Prabhakar,&nbsp;Aroonima Misra,&nbsp;Bhavika Rishi,&nbsp;Nidhi Chopra,&nbsp;Sanghamitra Ray","doi":"10.1111/ijlh.70013","DOIUrl":"10.1111/ijlh.70013","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Purpose</h3>\u0000 \u0000 <p>Pediatric hypereosinophilia (HE) is rare, and its evaluation is challenging due to diverse etiologies and limited access to advanced laboratory testing in low- and middle-income countries (LMICs).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We retrospectively analyzed five children with HE (absolute eosinophil count &gt; 5.0 × 10⁹/L) evaluated at a tertiary center. Clinical, hematologic, and molecular findings were reviewed, and diagnostic timelines were compared between clonal and non-clonal cases. Based on these observations, a tiered, laboratory-driven diagnostic pathway adapted for LMICs was developed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Five patients (median age: 6 years; range: 1.5–10 years) were included. The median absolute eosinophil count (AEC) at presentation was 12.8 × 10⁹/L (range: 6.5–21.5 × 10⁹/L). Three cases (60%) were clonal eosinophilia—one PDGFRB-rearranged myeloid/lymphoid neoplasm and two acute myeloid leukemia subtypes [AML with CBFB::MYH11, AML with RUNX1::RUNX1T1]—and two cases (40%) were non-clonal [one Hyper-IgE syndrome with secondary hemophagocytic lymphohistiocytosis (HLH), one secondary eosinophilia (drug-induced, phenytoin/phenobarbitone)]. Clonal cases demonstrated higher leukocyte counts, earlier bone marrow and molecular testing, and shorter median time to diagnosis (6 vs. 14 days), enabling prompt initiation of imatinib or AML-directed therapy with remission in all. In contrast, non-clonal HE required sequential exclusion of clonal disease, delaying immunosuppressive or drug-withdrawal strategies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>A structured, laboratory-driven algorithm beginning with blood counts, smear, and parasitic testing, and escalating to early bone marrow with cytogenetic/molecular studies for high-risk phenotypes, enabled timely identification of clonal HE while conserving resources in reactive cases. This LMIC-adapted pathway highlights laboratory turnaround time as a critical determinant of outcomes and provides a practical framework for pediatric HE evaluation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"48 1","pages":"150-164"},"PeriodicalIF":2.3,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145294758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning-Based Prediction of β-Thalassemia Trait Using Red Blood Cell Indices","authors":"Esra Paydaş Hataysal,&nbsp;Muslu Kazım Körez","doi":"10.1111/ijlh.70017","DOIUrl":"10.1111/ijlh.70017","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Anemia is a significant global health concern, with hypochromic microcytic anemia being the most common type. Among its causes, β-thalassemia trait (β-TT) and iron deficiency anemia (IDA) share similar hematological features, making differentiation challenging. We aimed to develop machine learning (ML) models using routine red blood cell (RBC) indices to distinguish β-TT, IDA, and healthy cases.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 8106 individuals (3378 healthy, 2696 IDA, 2032 β-TT) were included in this study. Six RBC parameters—RBC count, hemoglobin (HGB), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), MCH concentration (MCHC), and RBC distribution width (RDW-CV)—were used to train eXtreme gradient boosting (XGB), random forest (RF), and neural network (NN) models. The dataset was split into training (70%) and testing (30%) sets, with feature importance assessed via the Boruta algorithm. All statistical analyses were performed using R version 4.3.1 Statistical Language.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>All models achieved high accuracy (&gt; 97%), with RF demonstrating superior performance (97.86% accuracy, 99.71% AUC). The most significant features contributing to the models are MCH for the XGB algorithm, MCV for the RF algorithm, and HGB for the NN algorithm.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our findings demonstrate that ML-based models could offer a promising tool for improving β-TT detection, optimizing clinical workflows, and enhancing resource utilization in hematology.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"48 1","pages":"211-219"},"PeriodicalIF":2.3,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145305140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}