Activating autophagy promotes skin regeneration induced by mechanical stretch during tissue expansion.

IF 6.3 1区 医学 Q1 DERMATOLOGY Burns & Trauma Pub Date : 2024-02-07 eCollection Date: 2024-01-01 DOI:10.1093/burnst/tkad057
Jing Du, Wei Liu, Yajuan Song, Yu Zhang, Chen Dong, Shaoheng Xiong, Zhaosong Huang, Tong Wang, Jianke Ding, Qiang He, Zhou Yu, Xianjie Ma
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Abstract

Background: Tissue expansion, a technique in which skin regeneration is induced by mechanical stretch stimuli, is commonly used for tissue repair and reconstruction. In this study, we aimed to monitor the autophagy levels of expanded skin after the application of expansion stimuli and explore the effect of autophagy modulation on skin regeneration.

Methods: A rat scalp expansion model was established to provide a stable expanded skin response to mechanical stretch. Autophagy levels at different time points (6, 12, 24, 48 and 72 h after the last expansion) were detected via western blotting. The effect of autophagy regulation on skin regeneration during tissue expansion was evaluated via skin expansion efficiency assessment, western blotting, immunofluorescence staining, TUNEL staining and laser Doppler blood flow imaging.

Results: The autophagic flux reached its highest level 48 h after tissue expansion. Activating autophagy by rapamycin increased the area of expanded skin as well as the thicknesses of epidermis and dermis. Furthermore, activating autophagy accelerated skin regeneration during tissue expansion by enhancing the proliferation of cells and the number of epidermal basal and hair follicle stem cells, reducing apoptosis, improving angiogenesis, and promoting collagen synthesis and growth factor secretion. Conversely, the regenerative effects were reversed when autophagy was blocked.

Conclusions: Autophagy modulation may be a promising therapeutic strategy for improving the efficiency of tissue expansion and preventing the incidence of the complication of skin necrosis.

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在组织扩张过程中,激活自噬可促进机械拉伸诱导的皮肤再生。
背景:组织扩张是一种通过机械拉伸刺激诱导皮肤再生的技术,常用于组织修复和重建。本研究旨在监测扩张刺激后扩张皮肤的自噬水平,并探讨自噬调节对皮肤再生的影响:方法:建立大鼠头皮扩张模型,以提供稳定的扩张皮肤对机械拉伸的反应。方法:建立大鼠头皮扩张模型,提供稳定的扩张皮肤对机械拉伸的反应,并在不同时间点(最后一次扩张后 6、12、24、48 和 72 h)通过 Western 印迹检测自噬水平。通过皮肤扩张效率评估、Western 印迹、免疫荧光染色、TUNEL 染色和激光多普勒血流成像评估了组织扩张期间自噬调节对皮肤再生的影响:结果:自噬通量在组织扩张 48 小时后达到最高水平。雷帕霉素激活自噬作用可增加扩张皮肤的面积以及表皮和真皮的厚度。此外,在组织扩张过程中,激活自噬可通过增强细胞增殖、表皮基底细胞和毛囊干细胞的数量、减少细胞凋亡、改善血管生成、促进胶原蛋白合成和生长因子分泌,加速皮肤再生。相反,当自噬被阻断时,再生效果就会逆转:结论:自噬调节可能是提高组织扩张效率和预防皮肤坏死并发症发生的一种有前途的治疗策略。
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来源期刊
Burns & Trauma
Burns & Trauma 医学-皮肤病学
CiteScore
8.40
自引率
9.40%
发文量
186
审稿时长
6 weeks
期刊介绍: The first open access journal in the field of burns and trauma injury in the Asia-Pacific region, Burns & Trauma publishes the latest developments in basic, clinical and translational research in the field. With a special focus on prevention, clinical treatment and basic research, the journal welcomes submissions in various aspects of biomaterials, tissue engineering, stem cells, critical care, immunobiology, skin transplantation, and the prevention and regeneration of burns and trauma injuries. With an expert Editorial Board and a team of dedicated scientific editors, the journal enjoys a large readership and is supported by Southwest Hospital, which covers authors'' article processing charges.
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