Gleyce Ha de Souza, Marcia Sm Vaz, Joyce A Dos Santos Radai, Thiago L Fraga, Luana Rossato, Simone Simionatto
{"title":"Synergistic interaction of polymyxin B with carvacrol: antimicrobial strategy against polymyxin-resistant <i>Klebsiella pneumoniae</i>.","authors":"Gleyce Ha de Souza, Marcia Sm Vaz, Joyce A Dos Santos Radai, Thiago L Fraga, Luana Rossato, Simone Simionatto","doi":"10.2217/fmb-2023-0070","DOIUrl":null,"url":null,"abstract":"<p><p><b>Objective:</b> The antimicrobial activities of the synergistic combination of carvacrol and polymyxin B against polymyxin-resistant <i>Klebsiella pneumoniae</i> were evaluated. <b>Methods:</b> The methods employed checkerboard assays to investigate synergism, biofilm inhibition assessment and membrane integrity assay. In addition, the study included <i>in vivo</i> evaluation using a mouse infection model. <b>Results:</b> The checkerboard method evaluated 48 combinations, with 23 indicating synergistic action. Among these, carvacrol 10 mg/kg plus polymyxin B 2 mg/kg exhibited <i>in vivo</i> antimicrobial activity in a mouse model of infection, resulting in increased survival and a significant decrease in bacterial load in the blood. <b>Conclusion:</b> Polymyxin in synergy with carvacrol represents a promising alternative to be explored in the development of new antimicrobials.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"181-193"},"PeriodicalIF":2.5000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.2217/fmb-2023-0070","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/8 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: The antimicrobial activities of the synergistic combination of carvacrol and polymyxin B against polymyxin-resistant Klebsiella pneumoniae were evaluated. Methods: The methods employed checkerboard assays to investigate synergism, biofilm inhibition assessment and membrane integrity assay. In addition, the study included in vivo evaluation using a mouse infection model. Results: The checkerboard method evaluated 48 combinations, with 23 indicating synergistic action. Among these, carvacrol 10 mg/kg plus polymyxin B 2 mg/kg exhibited in vivo antimicrobial activity in a mouse model of infection, resulting in increased survival and a significant decrease in bacterial load in the blood. Conclusion: Polymyxin in synergy with carvacrol represents a promising alternative to be explored in the development of new antimicrobials.
期刊介绍：
Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
