The stem cell transcription factor ZFP296 transforms NIH3T3 cells and promotes anchorage-independent growth of cancer cells.

The International journal of developmental biology Pub Date : 2023-01-01 DOI:10.1387/ijdb.230143hk

Yumi Mizoue, Tomomi Ikeda, Takako Ikegami, Oleksandra Riabets, Yoshie Oishi, Morikuni Tobita, Hidenori Akutsu, Koichi Hattori, Beate Heissig, Hiroshi Koide

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Abstract

Cancer cells and embryonic stem (ES) cells share several biological properties, suggesting that some genes expressed in ES cells may play an important role in cancer cell growth. In this study, we investigated the possible role of zinc finger protein 296 (ZFP296), a transcription factor expressed in ES cells, in cancer development. First, we found that overexpression of Zfp296 in NIH3T3 mouse fibroblasts induced two phenomena indicative of cell transformation: enhanced proliferation under low-serum conditions and anchorage-independent growth. We also found that Zfp296 expression was upregulated in the tumor area of a mouse model of colon carcinogenesis. In addition, the expression levels of ZFP296 in various human cell lines were generally low in normal cells and relatively high in cancer cells. Finally, using a soft agar assay, we found that overexpression of ZFP296 promoted the anchorage-independent growth of cancer cells, while its knockdown had the opposite effect. Overall, these results suggest a possible role of the ES-specific transcription factor ZFP296 in cancer.

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干细胞转录因子ZFP296可转化NIH3T3细胞，并促进癌细胞的锚定依赖性生长。

癌细胞和胚胎干细胞（ES）具有一些共同的生物学特性，这表明ES细胞中表达的一些基因可能在癌细胞生长过程中发挥重要作用。在这项研究中，我们研究了锌指蛋白296（ZFP296）--一种在ES细胞中表达的转录因子--在癌症发展中可能扮演的角色。首先，我们发现在 NIH3T3 小鼠成纤维细胞中过表达 Zfp296 会诱导两种表明细胞转化的现象：低血清条件下增殖增强和锚定依赖性生长。我们还发现，Zfp296 在结肠癌小鼠模型的肿瘤区表达上调。此外，ZFP296 在各种人类细胞系中的表达水平在正常细胞中普遍较低，而在癌细胞中则相对较高。最后，通过软琼脂试验，我们发现过表达 ZFP296 会促进癌细胞的锚定依赖性生长，而敲除 ZFP296 则会产生相反的效果。总之，这些结果表明，ES特异性转录因子ZFP296可能在癌症中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The International journal of developmental biology

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