Complete genome sequence analysis of Bacillus velezensis A5, a promising biocontrol agent from the Pacific Ocean

IF 1.3 4区生物学 Q4 GENETICS & HEREDITY Marine genomics Pub Date : 2024-02-01 DOI:10.1016/j.margen.2024.101087

Shunhua Ji , Yin Tian , Guangxin Xu , Yiqiang Chen , Jingjing Li , Teng Long , Wei He , Jianqiang Fan , Xixiang Tang

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Abstract

Tobacco bacterial wilt (TBW) caused by Ralstonia solanacearum is a serious soil-borne disease, which seriously damages the growth of tobacco crops. Bacillus velezensis A5 was isolated from 3000 m deep-sea sediments of the Pacific Ocean, and was found to be antagonistic to TBW. Here, we report the complete genome sequence of strain A5, which has a 4,000,699-bp single circular chromosome with 3827 genes and a G + C content of 46.44%, 87 tRNAs, and 27 rRNAs. A total of 12 gene clusters were identified in the genome of strain A5, which were responsible for the biosynthesis of antibacterial compounds, including surfactin, bacillaene, fengycin, difficidin, bacillibactin, and bacilysin. Additionally, strain A5 was found to contain a series of genes related to the biosynthesis of carbohydrate-active enzymes and secreted proteins. Our results indicate that strain A5 can be considered a promising biocontrol agent against TBW in agricultural fields.

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来自太平洋的一种很有前途的生物控制剂--Bacillus velezensis A5 的全基因组序列分析

由 Ralstonia solanacearum 引起的烟草细菌性萎蔫病（TBW）是一种严重的土传病害，严重损害烟草作物的生长。从太平洋 3000 米深海沉积物中分离出的枯草芽孢杆菌 A5 被发现对 TBW 具有拮抗作用。在此，我们报告了菌株 A5 的完整基因组序列，它有一个 4,000,699-bp 的单环染色体，含有 3827 个基因，G + C 含量为 46.44%，87 个 tRNA 和 27 个 rRNA。在菌株 A5 的基因组中总共发现了 12 个基因簇，它们负责抗菌化合物的生物合成，包括表面活性素、杆菌烯、芬吉霉素、 difficidin、bacillibactin 和 bacilysin。此外，还发现菌株 A5 含有一系列与碳水化合物活性酶和分泌蛋白的生物合成有关的基因。我们的研究结果表明，菌株 A5 是一种很有前途的农田结核病生物控制剂。

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来源期刊

Marine genomics 生物-遗传学

CiteScore

3.60

自引率

5.30%

发文量

审稿时长

29 days

期刊介绍： The journal publishes papers on all functional and evolutionary aspects of genes, chromatin, chromosomes and (meta)genomes of marine (and freshwater) organisms. It deals with new genome-enabled insights into the broader framework of environmental science. Topics within the scope of this journal include: • Population genomics and ecology • Evolutionary and developmental genomics • Comparative genomics • Metagenomics • Environmental genomics • Systems biology More specific topics include: geographic and phylogenomic characterization of aquatic organisms, metabolic capacities and pathways of organisms and communities, biogeochemical cycles, genomics and integrative approaches applied to microbial ecology including (meta)transcriptomics and (meta)proteomics, tracking of infectious diseases, environmental stress, global climate change and ecosystem modelling.