Whole-Transcriptome Analysis Reveals the RNA Profiles in Mouse Bone Marrow Mesenchymal Stem Cells or Zebrafish Embryos After Exposure to Environmental Level of Tributyltin.

IF 2.7 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Bulletin of Environmental Contamination and Toxicology Pub Date : 2024-02-11 DOI:10.1007/s00128-024-03861-6

Ping Li, Cheng-Zhuang Chen, Ling Liu, Zhi-Hua Li

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Abstract

To understand the underlying molecular mechanisms, mouse bone marrow mesenchymal stem cells (BMSCs) and zebrafish embryos were exposed to the control group and Tributyltin (TBT) group (10 ng/L, environmental concentration) for 48 h, respectively. The expression profiles of RNAs were investigated using whole-transcriptome analysis in mouse BMSCs or zebrafish embryos after TBT exposure. For mouse BMSCs, the results showed 2,449 differentially expressed (DE) mRNAs, 59 DE miRNAs, 317 DE lncRNAs, and 15 circRNAs. Similarly, for zebrafish embryos, the results showed 1,511 DE mRNAs, 4 DE miRNAs, 272 DE lncRNAs, and 28 circRNAs. According to KEGG pathway analysis showed that DE RNAs were mainly associated with immune responses, signaling, and cellular interactions. Competing endogenous RNA (ceRNA) network analysis revealed that the regulatory network of miRNA-circRNA constructed in zebrafish embryos was more complex compared to that of mouse BMSCs.

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全转录组分析揭示小鼠骨髓间充质干细胞或斑马鱼胚胎暴露于环境水平的三丁基锡后的 RNA 图谱

为了解潜在的分子机制，小鼠骨髓间充质干细胞（BMSCs）和斑马鱼胚胎分别暴露于对照组和三丁基锡（TBT）组（10 ng/L，环境浓度）48小时。采用全转录组分析方法研究了暴露于 TBT 后小鼠 BMSCs 或斑马鱼胚胎中 RNA 的表达谱。结果显示，小鼠 BMSCs 有 2449 个差异表达（DE）的 mRNA、59 个差异表达的 miRNA、317 个差异表达的 lncRNA 和 15 个 circRNA。同样，在斑马鱼胚胎中，结果显示了 1,511 个 DE mRNA、4 个 DE miRNA、272 个 DE lncRNA 和 28 个 circRNA。KEGG 通路分析表明，DE RNA 主要与免疫反应、信号传导和细胞相互作用有关。竞争性内源性RNA（ceRNA）网络分析显示，斑马鱼胚胎中的miRNA-circRNA构建的调控网络比小鼠BMSCs的更为复杂。

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来源期刊

Bulletin of Environmental Contamination and Toxicology 环境科学-毒理学

CiteScore

5.60

自引率

3.70%

发文量

230

审稿时长

1.7 months

期刊介绍： The Bulletin of Environmental Contamination and Toxicology(BECT) is a peer-reviewed journal that offers rapid review and publication. Accepted submissions will be presented as clear, concise reports of current research for a readership concerned with environmental contamination and toxicology. Scientific quality and clarity are paramount.