Role of macrophages in trigeminal ganglia in ectopic orofacial pain associated with pulpitis

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Oral Biosciences Pub Date : 2024-03-01 DOI:10.1016/j.job.2024.02.001

Miki Sunaga, Yoshiyuki Tsuboi, Akihiro Kaizu, Masamichi Shinoda

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Abstract

Objectives

This study aimed to elucidate the role of macrophages in the trigeminal ganglia (TG) in developing pulpitis-associated ectopic orofacial pain.

Methods

Rats underwent maxillary pulp exposure, and Fluoro-Gold (FG) was administered in the ipsilateral whisker pad (WP). Head withdrawal threshold (HWT) upon mechanical stimulation of the WP was recorded, and liposomal clodronate clophosome-A (LCCA; macrophage depletion agent) was administered to the TG at three and four days after pulp exposure. Immunohistochemically, TG sections were stained with anti-Iba1 (a macrophage marker) and anti-Nav1.7 antibodies.

Results

Pulp exposure decreased HWT and increased the number of Iba1-IR cells near FG-labelled TG neurons. LCCA inhibited the decrease in HWT and stopped the increase of FG-labelled Nav1.7-IR TG neurons in the pulpitis group.

Conclusions

Activation of macrophages by pulpitis induces the overexpression of Nav1.7 in TG neurons receiving inputs from WP, resulting in pulpitis-induced ectopic facial mechanical allodynia.

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三叉神经节中的巨噬细胞在与牙髓炎相关的异位面痛中的作用。

研究目的本研究旨在阐明三叉神经节（TG）中的巨噬细胞在发生牙髓炎相关异位性口腔疼痛中的作用：方法：对大鼠进行上颌牙髓暴露，并在同侧须垫（WP）注射氟金（FG）。记录WP受到机械刺激时的头部退缩阈值（HWT），并在牙髓暴露后的第三天和第四天在TG中施用氯膦酸脂质体clophosome-A（LCCA；巨噬细胞消耗剂）。用抗Iba1（一种巨噬细胞标记物）和抗Nav1.7抗体对TG切片进行免疫组化染色：结果：果肉暴露降低了HWT，增加了FG标记的TG神经元附近的Iba1-IR细胞数量。LCCA抑制了牙髓炎组HWT的下降，并阻止了FG标记的Nav1.7-IR TG神经元的增加：结论：牙髓炎激活巨噬细胞可诱导接受 WP 输入的 TG 神经元过量表达 Nav1.7，从而导致牙髓炎诱发的异位面部机械痛。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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