Journal of Oral Biosciences最新文献

英文中文

Cyclic compression loading alters osteoarthritis-related gene expression in three-dimensionally cultured human articular chondrocytes via a different mechanism than interleukin-1β induction

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-17 DOI: 10.1016/j.job.2025.100653

Minami Hikida , Takashi Kanamoto , Yoshihito Tachi , Kosuke Ebina , Masahiro Nakajima , Ken Nakata

Objectives

Mechanical and inflammatory stimuli are key factors in the pathophysiology of osteoarthritis (OA). However, the effects of mechanical stimulation on joint tissues and cells at the molecular level and the mechanisms of interaction after stimulation with inflammatory cytokines remains uninvestigated.

Methods

Three-dimensional cyclic compression loading (CCL) was applied to human articular chondrocytes, and the expression of OA-related genes was analyzed using reverse transcription quantitative real-time polymerase chain reaction. Additionally, the effects of CCL after the chondrocytes were stimulated with interleukin (IL)-1β were evaluated. A DNA microarray assay was used to compare changes in gene expression after chondrocytes were stimulated with IL-1β and CCL was applied, and to search for pathways that are affected by CCL.

Results

CCL of 40 kPa significantly upregulated the expression of IL-8, cyclooxygenase (COX)-2, nerve growth factor, matrix metalloproteinase (MMP)-1, and MMP-3. Transcription of IL-8, COX-2, and MMP-3 was synergistically promoted by CCL and IL-1β. The top 10 pathways enriched in the Kyoto Encyclopedia of Genes and Genomes enrichment analysis of differentially expressed genes were not common in either group, except for the “cytokine-cytokine receptor interaction”. The “tumor necrosis factor signaling pathway” and the “nuclear factor-kappa B signaling pathway” in the IL-1β group and “cell cycle” and the “Hippo signaling pathway” in the CCL group were included.

Conclusions

Comprehensive gene expression analysis revealed that CCL-induced changes in gene expression were different to those induced by stimulation with IL-1β. Our results provide new insights into the involvement of mechanical stimulation in the pathogenesis of OA.

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引用次数: 0

Clinical, Radiographic, and Histological Features of Buccal Bifurcation Cysts: A Systematic Review to Aid Accurate Diagnosis and Treatment Decisions.

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-14 DOI: 10.1016/j.job.2025.100652

Matheus de Castro Costa, Rani Kanthan, Marina Lara de Carli, Felipe Fornias Sperandio

Objective: This systematic review delves into the nuanced landscape of buccal bifurcation cysts (BBCs), emphasizing their clinical significance amid the diagnostic challenges in oral and maxillofacial pathology. We trace the evolution of BBC classification from historical perspectives to its current status in the World Health Organization's classification system, aiming to equip dental professionals with crucial insights for accurate diagnosis and effective management.

Methods: This systematic review (PROSPERO: CRD42023405169) followed PRISMA guidelines to examine the epidemiological characteristics of BBCs. Observational studies were included, while reviews, meta-analyses, and experimental studies were excluded. A comprehensive search across five databases identified eligible studies. Two independent reviewers screened articles, resolving disagreements by consensus or a third reviewer. Data extraction included clinical, histological, and imaging findings. Risk of bias was assessed using Murad's framework for case reports/series and the Newcastle-Ottawa Scale for other study types, with studies rated as low, moderate, or high quality.

Results: The information presented here is crucial for preventing past treatment errors associated with BBC. In addition, this review confirms that BBCs predominantly affect the posterior mandible of pediatric patients and exhibit consistent clinical and histopathological features, aiding in their differentiation from similar maxillofacial lesions. Thus, well-informed clinicians should be able to diagnose BBC and make a proper treatment choice after familiarizing themselves with this review, which will ultimately lead to a favorable prognostic outcome and reduced risk of lesion recurrence.

Conclusion: This study provides a comprehensive analysis of BBC, aiming to enhance clinical understanding and ultimately improve patient care.

{"title":"Clinical, Radiographic, and Histological Features of Buccal Bifurcation Cysts: A Systematic Review to Aid Accurate Diagnosis and Treatment Decisions.","authors":"Matheus de Castro Costa, Rani Kanthan, Marina Lara de Carli, Felipe Fornias Sperandio","doi":"10.1016/j.job.2025.100652","DOIUrl":"https://doi.org/10.1016/j.job.2025.100652","url":null,"abstract":"<p><strong>Objective: </strong>This systematic review delves into the nuanced landscape of buccal bifurcation cysts (BBCs), emphasizing their clinical significance amid the diagnostic challenges in oral and maxillofacial pathology. We trace the evolution of BBC classification from historical perspectives to its current status in the World Health Organization's classification system, aiming to equip dental professionals with crucial insights for accurate diagnosis and effective management.</p><p><strong>Methods: </strong>This systematic review (PROSPERO: CRD42023405169) followed PRISMA guidelines to examine the epidemiological characteristics of BBCs. Observational studies were included, while reviews, meta-analyses, and experimental studies were excluded. A comprehensive search across five databases identified eligible studies. Two independent reviewers screened articles, resolving disagreements by consensus or a third reviewer. Data extraction included clinical, histological, and imaging findings. Risk of bias was assessed using Murad's framework for case reports/series and the Newcastle-Ottawa Scale for other study types, with studies rated as low, moderate, or high quality.</p><p><strong>Results: </strong>The information presented here is crucial for preventing past treatment errors associated with BBC. In addition, this review confirms that BBCs predominantly affect the posterior mandible of pediatric patients and exhibit consistent clinical and histopathological features, aiding in their differentiation from similar maxillofacial lesions. Thus, well-informed clinicians should be able to diagnose BBC and make a proper treatment choice after familiarizing themselves with this review, which will ultimately lead to a favorable prognostic outcome and reduced risk of lesion recurrence.</p><p><strong>Conclusion: </strong>This study provides a comprehensive analysis of BBC, aiming to enhance clinical understanding and ultimately improve patient care.</p>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":" ","pages":"100652"},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piezo1 promotes double-directional differentiation from human periodontal ligament progenitor cells.

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-14 DOI: 10.1016/j.job.2025.100651

Yuri Kono, Hiroshi Kajiya, Riko Nagano, Chisato Tominaga, Hidefumi Maeda, Tsugumi Fujita, Sachio Tamaoki

Objectives: Human periodontal ligament (PDL) progenitor cells (hPDLPCs) sense mechanical stress and differentiate into osteoblasts, cementoblasts, and fibroblasts during orthodontic tooth movement. The mechanosensitive ion channel Piezo1 has been known to be present in PDL tissues and is involved in mineralization during bone regeneration. However, the functional role and underlying mechanisms of Piezo1 in osteogenesis and cementogenesis are unknown. We hypothesize that Piezo proteins are expressed in and regulate the differentiation of hPDLPCs.

Methods: We examined the effects of Piezo1 activation, by agonist and mechanical stretching, on the expression of osteogenesis- and cementogenesis-related molecules in hPDLPCs using RT-PCR, western blotting, and immunofluorescence methods.

Results: hPDLPCs showed calcium influx in Piezo1 and Piezo2, but not in TRPV4 and its channels. In hPDLPCs, the Piezo1 agonist Yoda1 significantly upregulated osteogenesis- and cementogenesis-related molecules through the Ca²⁺/CREB pathway. To investigate the role of Piezo1 in hPDLPC-mediated differentiation, knockout (KO) of Piezo1 in hPDLPCs was generated; significant downregulation of osteogenesis- and cementogenesis-related molecules was observed in KO hPDLPCs. Furthermore, Piezo1 enhanced the mineralization of hPDLPCs.

Conclusions: hPDLPCs expressed Piezo1 and Piezo2. Yoda1, Piezo1 agonist, significantly upregulated osteogenesis- and cementogenesis-related molecules through the Ca²⁺/CREB signaling pathway.

{"title":"Piezo1 promotes double-directional differentiation from human periodontal ligament progenitor cells.","authors":"Yuri Kono, Hiroshi Kajiya, Riko Nagano, Chisato Tominaga, Hidefumi Maeda, Tsugumi Fujita, Sachio Tamaoki","doi":"10.1016/j.job.2025.100651","DOIUrl":"https://doi.org/10.1016/j.job.2025.100651","url":null,"abstract":"<p><strong>Objectives: </strong>Human periodontal ligament (PDL) progenitor cells (hPDLPCs) sense mechanical stress and differentiate into osteoblasts, cementoblasts, and fibroblasts during orthodontic tooth movement. The mechanosensitive ion channel Piezo1 has been known to be present in PDL tissues and is involved in mineralization during bone regeneration. However, the functional role and underlying mechanisms of Piezo1 in osteogenesis and cementogenesis are unknown. We hypothesize that Piezo proteins are expressed in and regulate the differentiation of hPDLPCs.</p><p><strong>Methods: </strong>We examined the effects of Piezo1 activation, by agonist and mechanical stretching, on the expression of osteogenesis- and cementogenesis-related molecules in hPDLPCs using RT-PCR, western blotting, and immunofluorescence methods.</p><p><strong>Results: </strong>hPDLPCs showed calcium influx in Piezo1 and Piezo2, but not in TRPV4 and its channels. In hPDLPCs, the Piezo1 agonist Yoda1 significantly upregulated osteogenesis- and cementogenesis-related molecules through the Ca<sup>2+</sup>/CREB pathway. To investigate the role of Piezo1 in hPDLPC-mediated differentiation, knockout (KO) of Piezo1 in hPDLPCs was generated; significant downregulation of osteogenesis- and cementogenesis-related molecules was observed in KO hPDLPCs. Furthermore, Piezo1 enhanced the mineralization of hPDLPCs.</p><p><strong>Conclusions: </strong>hPDLPCs expressed Piezo1 and Piezo2. Yoda1, Piezo1 agonist, significantly upregulated osteogenesis- and cementogenesis-related molecules through the Ca<sup>2+</sup>/CREB signaling pathway.</p>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":" ","pages":"100651"},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Androgen suppression protects against hyposalivation and salivary gland damage in mice with type 2 diabetes

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-06 DOI: 10.1016/j.job.2025.100646

Ana Lilia García-Hernández, Nancy Cruz-Mendoza, Gerardo Arturo Rueda-Cortez, Saúl Ernesto Cifuentes-Mendiola

Objective

Hyposalivation is one of the most common oral complications of type 2 diabetes (T2D). Sex hormone levels, which have been associated with hyposalivation, salivary gland atrophy, and inflammation, can be altered in T2D. However, the relationship between androgen levels and hyposalivation in the context of T2D is unknown. Therefore, this study investigated the role of gonadal androgen suppression on the function and histomorphometry of salivary glands in mice with T2D.

Methods

Four-week-old male C57BL/6 mice were divided into four groups: control, orchiectomy (ORQx), T2D, and ORQx-T2D. Orchiectomy was performed at eight weeks of age, and T2D was induced using a high-calorie diet and low-dose streptozotocin. At 20 weeks of age, the blood glucose levels, saliva secretion and quality, and serum testosterone were measured. The parotid and submandibular glands were retrieved, processed for histology, and sections were stained with hematoxylin and eosin, Sirius Red or immunohistochemically stained for α-amylase, interleukin (IL)-1, IL-6, IL-10, IL-17, and tumor necrosis factor-α.

Results

Mice with T2D exhibited decreased saliva secretion and quality, reduced α-amylase expression, and the number of acini. They also developed glandular fibrosis and acinar hypertrophy, along with increased in proinflammatory cytokines in both salivary glands. Androgen suppression in mice with T2D reduced hyperglycemia, normalized saliva secretion, decreased glandular fibrosis and acinar hypertrophy, increased α-amylase expression, and reduced proinflammatory cytokine expression in both glands.

Conclusions

Androgen suppression in mice with T2D reduces the development of hyposalivation and histomorphometric changes in the parotid and submandibular glands by modulating the inflammatory microenvironment.

{"title":"Androgen suppression protects against hyposalivation and salivary gland damage in mice with type 2 diabetes","authors":"Ana Lilia García-Hernández, Nancy Cruz-Mendoza, Gerardo Arturo Rueda-Cortez, Saúl Ernesto Cifuentes-Mendiola","doi":"10.1016/j.job.2025.100646","DOIUrl":"10.1016/j.job.2025.100646","url":null,"abstract":"<div><h3>Objective</h3><div>Hyposalivation is one of the most common oral complications of type 2 diabetes (T2D). Sex hormone levels, which have been associated with hyposalivation, salivary gland atrophy, and inflammation, can be altered in T2D. However, the relationship between androgen levels and hyposalivation in the context of T2D is unknown. Therefore, this study investigated the role of gonadal androgen suppression on the function and histomorphometry of salivary glands in mice with T2D.</div></div><div><h3>Methods</h3><div>Four-week-old male C57BL/6 mice were divided into four groups: control, orchiectomy (ORQx), T2D, and ORQx-T2D. Orchiectomy was performed at eight weeks of age, and T2D was induced using a high-calorie diet and low-dose streptozotocin. At 20 weeks of age, the blood glucose levels, saliva secretion and quality, and serum testosterone were measured. The parotid and submandibular glands were retrieved, processed for histology, and sections were stained with hematoxylin and eosin, Sirius Red or immunohistochemically stained for α-amylase, interleukin (IL)-1, IL-6, IL-10, IL-17, and tumor necrosis factor-α.</div></div><div><h3>Results</h3><div>Mice with T2D exhibited decreased saliva secretion and quality, reduced α-amylase expression, and the number of acini. They also developed glandular fibrosis and acinar hypertrophy, along with increased in proinflammatory cytokines in both salivary glands. Androgen suppression in mice with T2D reduced hyperglycemia, normalized saliva secretion, decreased glandular fibrosis and acinar hypertrophy, increased α-amylase expression, and reduced proinflammatory cytokine expression in both glands.</div></div><div><h3>Conclusions</h3><div>Androgen suppression in mice with T2D reduces the development of hyposalivation and histomorphometric changes in the parotid and submandibular glands by modulating the inflammatory microenvironment.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100646"},"PeriodicalIF":2.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Letter to the editor, “Mirror-polished ceria-stabilized zirconia/alumina nanocomposite enhances gingival junctional epithelial cell adhesion” [J Oral Biosci, Volume 67, 2025. DOI: 10.1016/j.job.2024.100593]

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-04 DOI: 10.1016/j.job.2025.100647

Nicholas G. Fischer

引用次数: 0

Antimicrobial effects of great salt lake mineral salts on oral pathogenic bacteria: Implications for oral care

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-03 DOI: 10.1016/j.job.2025.100633

Inori Inui , Atsushi Iwatsuki , Yoshie Yoshioka , Manabu Habu , Wataru Ariyoshi , Ryota Yamasaki

This study investigated the antimicrobial effects of purified natural mineral salts from the Great Salt Lake on oral pathogenic bacteria. Salts rich in sodium, calcium, potassium, and magnesium effectively inhibit the growth and biofilm formation of cariogenic and periodontopathogenic bacteria at lower concentrations than does sodium chloride. These findings suggest the potential applications of these salts in oral care products such as toothpaste and mouthwash.

引用次数: 0

Reactivation of Epstein-Barr virus by n-butyric acid from Pseudoramibacter alactolyticus induces inflammatory cytokines in periapical granulomas 溶乳假杆菌产生的正丁酸使 Epstein-Barr 病毒重新活化，诱导根尖周炎肉芽肿中的炎性细胞因子。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.10.001

Taiki Miyata , Osamu Takeichi , Kenichi Imai , Masayuki Okano , Seiya Inoue , Takuya Yasukawa , Yusuke Suzuki

Objectives

This study investigates whether latent Epstein-Barr virus (EBV) can be reactivated by n-butyric acid from Pseudoramibacter alactolyticus, and if such reactivation induces expression of interleukin (IL)-1β and IL-6 in periapical granulomas.

Methods

We analyzed periapical granulomas and healthy gingival tissues to detect the presence of EBV and P. alactolyticus. The concentration of n-butyric acid in P. alactolyticus culture supernatants was measured. BZLF-1 luciferase assays were conducted with or without these supernatants. Immunohistochemical detection of ZEBRA-, IL-1β-, and IL-6-expressing cells was performed in the tissue samples. Additionally, mRNA expression levels of BZLF-1, IL-1β, and IL-6 were quantified and statistically analyzed for correlation. The expression of these mRNAs was also measured in Daudi cells treated with or without the culture supernatants.

Results

Both EBV and P. alactolyticus were detected in periapical granulomas, but not in healthy tissues. The concentration of n-butyric acid in the culture supernatants was ∼3.58 mmol/L. BZLF-1 luciferase activity in the presence of the culture supernatants was comparable to that of commercially available butyric acid, whereas no activity was detected without the supernatants. Cells expressing ZEBRA co-expressed IL-1β and IL-6. The mRNA levels of IL-1β and IL-6 in periapical granulomas were correlated with BZLF-1 mRNA levels. Daudi cells treated with the culture supernatants expressed BZLF-1, IL-1β, and IL-6 mRNA, while those without the supernatants did not.

Conclusions

The study concludes that EBV can be reactivated by n-butyric acid produced by P. alactolyticus, leading to the induction of IL-1β and IL-6 expression in periapical granulomas.

研究目的本研究探讨了潜伏的爱泼斯坦-巴氏病毒（EBV）能否被溶乳假杆菌的正丁酸重新激活，以及这种重新激活能否诱导根尖周炎肉芽肿中白细胞介素（IL）-1β和IL-6的表达：我们分析了根尖周炎肉芽肿和健康牙龈组织，以检测是否存在 EBV 和溶乳杆菌。测量溶乳杆菌培养上清液中的正丁酸浓度。用或不用这些上清液进行 BZLF-1 荧光素酶检测。对组织样本中的 ZEBRA-、IL-1β 和 IL-6 表达细胞进行免疫组化检测。此外，还对 BZLF-1、IL-1β 和 IL-6 的 mRNA 表达水平进行了量化和相关性统计分析。在使用或不使用培养上清液处理的 Daudi 细胞中也检测了这些 mRNA 的表达：结果：在根尖周炎肉芽肿中检测到 EBV 和溶乳杆菌，而在健康组织中未检测到。培养上清液中的正丁酸浓度为 3.58 mmol/L。在有培养上清存在的情况下，BZLF-1荧光素酶的活性与市售丁酸的活性相当，而在没有培养上清的情况下则检测不到任何活性。表达 ZEBRA 的细胞同时表达 IL-1β 和 IL-6。根尖周炎肉芽肿中 BZLF-1、IL-1β 和 IL-6 的 mRNA 水平与 EBV DNA 拷贝数相关。用培养上清液处理的 Daudi 细胞表达 BZLF-1、IL-1β 和 IL-6 mRNA，而未用培养上清液处理的细胞则不表达：本研究得出结论：EBV 可被溶乳杆菌产生的正丁酸重新激活，从而诱导根尖周炎肉芽肿中 IL-1β 和 IL-6 的表达。

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引用次数: 0

Decline in stimulus responsiveness of secretory granules in salivary glands with age 唾液腺分泌颗粒对刺激的反应能力随年龄增长而下降。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100583

Miyuki Toda , Megumi Yokoyama , Osamu Katsumata-Kato , Junko Fujita-Yoshigaki

Objectives

Secretory granules produced by salivary acinar cells accumulate if secretory stimulation is suppressed. Aged and deteriorated secretory granules can cause tissue damage because of abnormal secretion and/or intracellular leakage. To elucidate the deterioration process, we characterized the changes in the stimulus responsiveness of secretory granules using HaloTag technology.

Methods

We established a system in which HaloTag-fused cystatin D, a salivary protein, was transported to the secretory granules of rat parotid acinar cells in primary culture. HaloTags can be labeled with cell-permeable ligands conjugated to fluorescent dyes in living cells. To observe the new and old secretory granules, the cells were labeled with two HaloTag ligands conjugated to different fluorescent dyes at different times. We measured the secretion rates of newly synthesized and old HaloTag-fused proteins in the absence and presence of isoproterenol, a β-adrenergic agonist, at 3 and 6 h after initial labeling.

Results

Sequential labeling of HaloTag-fused proteins with two different dyes enabled the discrimination between new and old secretory granules. The secretory responsiveness of the proteins synthesized within 3 h to isoproterenol was higher than that of proteins synthesized earlier. However, there was no significant difference in the responsiveness between the new and old proteins at 6 h after initial labeling.

Conclusion

New secretory granules have a higher sensitivity to stimulants than older ones and that their response declines over time.

目的：如果分泌刺激受到抑制，唾液腺尖突细胞产生的分泌颗粒就会积聚。老化和变质的分泌颗粒会因异常分泌和/或细胞内渗漏而造成组织损伤。为了阐明退化过程，我们利用 HaloTag 技术描述了分泌颗粒对刺激反应性的变化：我们建立了一个系统，在该系统中，融合了 HaloTag 的胱抑素 D（一种唾液蛋白）被转运到原代培养的大鼠腮腺腺细胞的分泌颗粒中。在活细胞中，HaloTags 可以用与荧光染料连接的细胞渗透性配体标记。为了观察新旧分泌颗粒，我们在不同时间用两种与不同荧光染料连接的 HaloTag 配体标记细胞。在没有和有β肾上腺素能激动剂异丙肾上腺素的情况下，我们测量了初次标记后3小时和6小时新合成和旧的HaloTag融合蛋白的分泌率：结果：用两种不同的染料对融合了HaloTag的蛋白质进行连续标记可区分新旧分泌颗粒。3小时内合成的蛋白质对异丙肾上腺素的分泌反应性高于较早合成的蛋白质。结论：新的分泌颗粒对异丙肾上腺素的分泌反应性更高：结论：新的分泌颗粒对刺激物的敏感性高于老的分泌颗粒，而且它们的反应会随着时间的推移而下降。

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引用次数: 0

ED-71 promotes osseointegration of titanium implants in a rat model of GIOP by alleviating the effects of dexamethasone on bone remodeling in a SIRT1-dependent manner ED-71 可通过 SIRT1 依赖性方式减轻地塞米松对骨重塑的影响，从而促进钛植入物在大鼠 GIOP 模型中的骨结合。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.10.003

Chunying Li , Pengfei Xue , Guanglin Duan , Ailing Song , Runbing Zhai , Jie Ma , Minqi Li

Objective

Glucocorticoid-induced osteoporosis (GIOP), a common complication of glucocorticoid usage, plays a critical role in the success of dental implant restoration by affecting osseointegration. Eldecalcitol (ED-71) prevents GIOP; however, its role in the osseointegration of implants under GIOP conditions remains elusive.

Methods

Dexamethasone was used to establish a rat model of GIOP. Subsequently, mini-implant surgery was performed on the femur. GIOP rats were administered ED-71 via gavage to assess its role in the osseointegration of titanium implants under GIOP conditions. MC3T3-E1 and RAW264.7 cells were utilized to explore the molecular mechanism of ED-71 in ameliorating disorder of bone remodeling caused by dexamethasone.

Results

The administration of ED-71 promoted the formation of newly formed woven bone and the resolution of inflammation around titanium implants. In vitro experiments indicated that ED-71 ameliorated dexamethasone-induced dysfunction of osteoblasts and osteoclasts by increasing the expression level of sirtuin 1 (SIRT1). Inhibition of SIRT1 by selisistat counteracts the regulatory effects of ED-71 on dexamethasone-induced disorder of bone remodeling. Molecular docking and Western blotting revealed that the neurogenic locus notch homolog protein and nuclear factor kappa B signaling pathways are essential for the effects of ED-71 on dexamethasone-induced disorder of bone remodeling.

Conclusion

ED-71 promoted implant osseointegration in a rat model of GIOP by alleviating the effects of dexamethasone on bone remodeling in a SIRT1-dependent manner.

目的：糖皮质激素诱导的骨质疏松症（GIOP）是使用糖皮质激素的常见并发症，它通过影响骨结合对牙科种植修复的成功起着至关重要的作用。艾地卡骨化醇（ED-71）可预防 GIOP，但它在 GIOP 条件下对种植体骨结合的作用仍不明确：方法：使用地塞米松建立大鼠 GIOP 模型。方法：使用地塞米松建立 GIOP 大鼠模型，随后在股骨上进行微型植入手术。给 GIOP 大鼠灌胃 ED-71，以评估其在 GIOP 条件下对钛植入物骨结合的作用。利用 MC3T3-E1 和 RAW264.7 细胞探讨 ED-71 改善地塞米松引起的骨重塑紊乱的分子机制：结果：服用ED-71可促进新形成的编织骨的形成，并缓解钛种植体周围的炎症。体外实验表明，ED-71通过提高sirtuin 1（SIRT1）的表达水平，改善了地塞米松诱导的成骨细胞和破骨细胞功能障碍。塞利司他对SIRT1的抑制抵消了ED-71对地塞米松诱导的骨重塑障碍的调节作用。分子对接和Western印迹显示，ED-71对地塞米松诱导的骨重塑障碍的影响离不开神经原位点缺口同源蛋白和核因子卡巴B信号通路：结论：ED-71以SIRT1依赖的方式减轻了地塞米松对骨重塑的影响，从而促进了大鼠GIOP模型中种植体的骨结合。

{"title":"ED-71 promotes osseointegration of titanium implants in a rat model of GIOP by alleviating the effects of dexamethasone on bone remodeling in a SIRT1-dependent manner","authors":"Chunying Li , Pengfei Xue , Guanglin Duan , Ailing Song , Runbing Zhai , Jie Ma , Minqi Li","doi":"10.1016/j.job.2024.10.003","DOIUrl":"10.1016/j.job.2024.10.003","url":null,"abstract":"<div><h3>Objective</h3><div>Glucocorticoid-induced osteoporosis (GIOP), a common complication of glucocorticoid usage, plays a critical role in the success of dental implant restoration by affecting osseointegration. Eldecalcitol (ED-71) prevents GIOP; however, its role in the osseointegration of implants under GIOP conditions remains elusive.</div></div><div><h3>Methods</h3><div>Dexamethasone was used to establish a rat model of GIOP. Subsequently, mini-implant surgery was performed on the femur. GIOP rats were administered ED-71 via gavage to assess its role in the osseointegration of titanium implants under GIOP conditions. MC3T3-E1 and RAW264.7 cells were utilized to explore the molecular mechanism of ED-71 in ameliorating disorder of bone remodeling caused by dexamethasone.</div></div><div><h3>Results</h3><div>The administration of ED-71 promoted the formation of newly formed woven bone and the resolution of inflammation around titanium implants. In vitro experiments indicated that ED-71 ameliorated dexamethasone-induced dysfunction of osteoblasts and osteoclasts by increasing the expression level of sirtuin 1 (SIRT1). Inhibition of SIRT1 by selisistat counteracts the regulatory effects of ED-71 on dexamethasone-induced disorder of bone remodeling. Molecular docking and Western blotting revealed that the neurogenic locus notch homolog protein and nuclear factor kappa B signaling pathways are essential for the effects of ED-71 on dexamethasone-induced disorder of bone remodeling.</div></div><div><h3>Conclusion</h3><div>ED-71 promoted implant osseointegration in a rat model of GIOP by alleviating the effects of dexamethasone on bone remodeling in a SIRT1-dependent manner.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100571"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MANDI-code: A coding system for the human mandible MANDI-Code：人类下颌骨编码系统。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100584

Doha Abualhija , Julieta Gómez García-Donas , Simon Shepherd , Ranya Al Ghazi , Scheila Manica

Background

The mandible provides valuable insights into its biological identity. However, the existence of several terminologies for mandibular measurements and inconsistent language can lead to misinterpretation, confusion, and miscommunication.

Highlight

A standardised set of anatomical points, planes, and measurements would assist with these issues and ensure reproducibility and comparability.

Conclusion

The proposed coding system offers a comprehensive approach for professionals and researchers in dentistry, archaeology, and anthropology.

背景：下颌骨为了解生物特征提供了宝贵的信息。然而，下颌骨测量存在多种术语和不一致的语言，可能导致误解、混淆和误传：重点：一套标准化的解剖点、平面和测量方法将有助于解决这些问题，并确保可重复性和可比性：建议的编码系统为牙科、考古学和人类学领域的专业人员和研究人员提供了一种全面的方法。

引用次数: 0

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