Usefulness of a new immunochromatographic assay using fluorescent silica nanoparticles for serodiagnosis of Thai patients with amebiasis.

IF 1.9 Q3 PHARMACOLOGY & PHARMACY Drug Discoveries and Therapeutics Pub Date : 2024-03-20 Epub Date: 2024-02-15 DOI:10.5582/ddt.2023.01102
Azumi Kakino, Urassaya Pattanawong, Napaporn Kuamsab, Tatsuya Imai, Chaturong Putaporntip, Satomi Asai, Xunjia Cheng, Somchai Jongwutiwes, Hiroshi Tachibana
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Abstract

A fluorescence immunochromatography (FIC) kit was developed recently using fluorescent silica nanoparticles coated with a recombinant C-terminal fragment of the surface lectin intermediate subunit (C-Igl) of Entamoeba histolytica to establish rapid serodiagnosis of amebiasis. We further evaluated the system using serum samples from 52 Thai patients with amebiasis. Of the patients, 50 (96%) tested positive using FIC. The samples were also tested using enzyme-linked immunosorbent assay (ELISA) with C-Igl as the antigen. Two samples were negative on ELISA but positive on FIC. The correlation coefficient between the fluorescence intensity using FIC and the optical density value using ELISA was 0.5390, indicating a moderate correlation between the two tests. Serum samples from 20 patients with malaria and 22 patients with Clostridioides difficile infection were also tested using FIC. The false-positive rates were 4/20 (20%) and 1/22 (4%) in patients with malaria and C. difficile infection, respectively. Combining the data from the present study with our previous study, the sensitivity and specificity of FIC were determined to be 98.5% and 95.2%, respectively. The results of the 50 samples were studied using a fluorescence scope and a fluorescence intensity reader, and the findings were compared. Disagreements were found in only two samples showing near-borderline fluorescence intensity, indicating that the use of scope was adequate for judging the results. These results demonstrate that FIC is a simple and rapid test for the serodiagnosis of amebiasis.

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使用荧光硅纳米粒子的新型免疫层析检测法在泰国阿米巴病患者血清诊断中的实用性。
最近,我们开发了一种荧光免疫层析(FIC)试剂盒,该试剂盒使用了涂有恩塔莫阿米巴组织溶解虫表面凝集素中间亚基(C-Igl)重组 C 端片段的荧光硅纳米粒子,可用于阿米巴病的快速血清诊断。我们使用 52 名泰国阿米巴病患者的血清样本对该系统进行了进一步评估。其中 50 名患者(96%)的 FIC 检测结果呈阳性。我们还使用以 C-Igl 为抗原的酶联免疫吸附试验(ELISA)对样本进行了检测。有两个样本在 ELISA 检测中呈阴性,但在 FIC 检测中呈阳性。使用 FIC 检测的荧光强度与使用 ELISA 检测的光密度值之间的相关系数为 0.5390,表明这两种检测方法之间存在中度相关性。20 名疟疾患者和 22 名艰难梭菌感染患者的血清样本也用 FIC 进行了检测。疟疾和艰难梭菌感染患者的假阳性率分别为 4/20(20%)和 1/22 (4%)。结合本研究和我们之前的研究数据,FIC 的灵敏度和特异度分别为 98.5% 和 95.2%。使用荧光显微镜和荧光强度阅读器对 50 份样本的结果进行了研究和比较。结果发现,只有两个样本的荧光强度接近边界线,表明使用荧光显微镜足以判断结果。这些结果表明,FIC 是一种用于阿米巴病血清诊断的简单而快速的检测方法。
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来源期刊
Drug Discoveries and Therapeutics
Drug Discoveries and Therapeutics PHARMACOLOGY & PHARMACY-
CiteScore
3.20
自引率
3.20%
发文量
51
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