Drug Discoveries and Therapeutics最新文献

Age-related hearing loss (ARHL) has been closely linked to genetic factors, with studies identifying the p.V37I mutation in the GJB2 gene as a potential contributor to ARHL. To investigate this, we generated a humanized p.V37I mutant mouse model and performed auditory brainstem response (ABR) testing, cochlear morphology assessments, and transcriptional sequence of mutant and wild-type (WT) mice at different ages. Our results indicated that this kind of GJB2 mutation does not lead to cochlear developmental abnormalities, and aging mutant mice exhibit only mild hearing loss compared to WT mice, without significant cochlear morphological differences. However, transcriptional analyses revealed substantial differences between mutant and WT mice. GO enrichment analysis of the DEGs between aging mutant and WT mice highlights significant enrichment in biological processes related to neural and sensory functions. Notably enriched terms include "neuron-to-neuron synapse," "immune response-activating signaling pathway," "regulation of synapse structure or activity," and "sensory perception of sound." These findings suggest that the p.V37I mutation in aging mice affects synaptic and calcium signaling pathways, as well as sensory system development. Despite these molecular changes, cochlear function remains normal in early life; however, as the mice age, hearing loss accelerates, likely due to a diminished capacity for gene-mediated protection against external stimuli.

The aim of this study was to determine the time-dependent effects of the prickly pear (Nopalea cochenillifera) on the intestinal environment and immune function in BALB/c mice, which exhibit a predominant T helper 2 immune response. Five-week-old female BALB/c mice were divided into a control group and groups fed diets supplemented with 5% or 10% N. cochenillifera powder (NCP). Blood and feces were collected every 7 days. On day 28, in addition to blood and feces, the small intestine, cecum, and large intestine were collected. The cecum content weight, cecum content pH, and fecal mucin were examined. Serum antibody levels (total Immunoglobulin (Ig)M) were lower on days 14 and 21 in the 10% NCP group than in the control and 5% NCP groups. Total serum IgG levels were higher at all time points and total IgA levels were higher on days 7, 14, 21 and 28 in the 5% NCP and 10% NCP groups than in the control group. Total fecal IgA levels were lower in the 5% NCP and 10% NCP groups than in the control group from 14 days. Fecal mucin contents were higher in the 10% NCP group than in the control group from 7 days. These results suggest that N. cochenillifera supplementation exerts time-dependent effects on serum antibodies and the intestinal environment in BALB/c mice. In particular, NCP at 10% may enhance the intestinal mucosal barrier function and promote systemic immune responses. Further mechanistic studies are needed to determine the effects on immune responses.

Uncomplicated urinary tract infection (uUTI) is a common bacterial infection in women. While the condition typically has a favorable prognosis, the widespread use of antibiotics has led to increasingly bacterial resistance, reducing the efficacy of traditional antibiotics. On March 25, 2025, the US Food and Drug Administration approved gepotidacin for the treatment of uUTIs caused by susceptible bacteria in female adult and pediatric patients 12 years of age and older weighing at least 40 kg. Gepotidacin is the first triazaacenaphthylene antibiotic approved for clinical use. It selectively binds to and inhibits both bacterial DNA gyrase and topoisomerase IV, disrupting bacterial DNA replication through a unique mechanism and thereby killing the pathogen. Results of clinical trials indicated the non-inferiority of gepotidacin compared to the current standard therapy, nitrofurantoin. The most frequently reported adverse reaction to gepotidacin was mild to moderate diarrhea. The approval of gepotidacin represents a progress in antibiotic innovation, offering novel perspectives on drug development while spurring global efforts to tackle the escalating challenge of antimicrobial resistance.

Hemophilia is a coagulation disorder caused by deficiencies in clotting factors and is primarily classified into hemophilia A (factor VIII deficiency) and hemophilia B (factor IX deficiency). Current treatment relies predominantly on replacement therapy, where patients receive clotting factor concentrates either episodically or prophylactically to achieve hemostasis or mitigate bleeding risks. Despite its efficacy, this approach presents limitations, including suboptimal treatment accessibility due to high costs, reduced patient compliance from frequent intravenous administration, and therapeutic failure in patients developing plasma inhibitors. These challenges underscore the need for novel therapeutics addressing unmet clinical demands. On March 28, 2025, the US Food and Drug Administration (FDA) approved fitusiran, a small interfering RNA (siRNA) therapeutic for hemophilia. This first-in-class agent demonstrates pan-hemophilia efficacy by targeting antithrombin to enhance thrombin activity, irrespective of factor VIII/IX deficiency status or plasma inhibitor presence. By pioneering a mechanism of antithrombin suppression, enabling sustained therapeutic action, and facilitating precision monitoring protocols, fitusiran has the potential to redefine hemophilia treatment paradigms.

Glucagon-like peptide-1 receptor agonist (GLP-1RA) has attracted attention owing to its hypoglycemic and weight-loss effects, and various dosage forms are available in the market. Recently, glucose-dependent insulin secretion-stimulating polypeptide (GIP), an incretin hormone in the same family as GLP-1, has attracted attention, and tirzepatide, a GIP/GLP-1RA, has been launched. Given the short duration of tirzepatide on the market and the fact that its therapeutic effects in patients of different ages have not been reported, we conducted this study. HbA1c improved significantly in patients aged ≥ 65 years, whereas HbA1c, weight, and LDL cholesterol also improved significantly in patients aged ≤ 64 years when compared between the beginning of use and 3 months following use. Tirzepatide has a hypoglycemic effect regardless of age; however, its weight loss effect may be more pronounced in younger age groups. Therefore, optimal diabetes treatment with tirzepatide should consider the age and weight of patients.

Expectations for pharmacists' contribution and involvement in pressure ulcer pharmacotherapy are growing. This study aimed to not only clarify the effects of incorporating practical training on the use of topical medications into clinical preparatory education but also examine the relationship between students' aspiration-or lack thereof-to pursue a career as a pharmacist. The percentage of positive responses to the questionnaire survey on knowledge, skills, and attitudes toward pressure ulcer treatment was significantly higher after the lecture than before and highest after the practical training. In addition, students who aspired to become pharmacists tended to provide a higher percentage of positive responses after the lecture. However, the gap between aspiring and non-aspiring pharmacists tended to narrow after practical training. These results suggest that incorporating practical training on pressure ulcer treatment into lectures improves students' knowledge, skills, and attitudes toward its treatment. Including practical training in pressure ulcer education may compensate for the difference in the educational effect of lectures between students who aspire to become pharmacists and those who do not, thereby improving the educational effect for the latter.

Aeromonas hydrophila is a significant pathogenic bacterium in aquaculture and the ornamental fish industry, causing lethal infections in fish and contributing to rising drug resistance. This leads to substantial economic losses and underscores the urgent need for new treatments and infection controls. However, the lack of a simple, sensitive infection model has hindered studies on the pathogenicity of A. hydrophila and therapeutic evaluation. This study introduces the silkworm (Bombyx mori) as a highly sensitive and cost-effective infection model for A. hydrophila. Using a strain isolated from diseased Wakins (goldfish), the pathogenicity of A. hydrophila was confirmed in silkworms, which exhibited a much lower median lethal dose (LD₅₀ = 0.3 CFU/larva) compared to Wakins (LD₅₀ = 5.1 × 10⁶ CFU/g body weight). This demonstrates the silkworm's higher sensitivity to A. hydrophila. The in vivo efficacy of three antibiotics (gentamicin, kanamycin, and tetracycline) was also tested. Gentamicin and kanamycin prolonged survival in both models, while tetracycline also showed efficacy in both models, though its effect was weaker in the silkworm model. This highlights the silkworm model's utility in evaluating bactericidal agents against A. hydrophila. This model addresses key limitations of traditional fish infection models, including low sensitivity, long experimental durations, and high costs. The silkworm-based method enables efficient investigation of A. hydrophila pathogenicity and rapid screening of potential treatments, accelerating the development of new therapeutic strategies for aquaculture and beyond.

This article was to summarize the treatment course and prognosis of immunocompetent and immunocompromised patients with pulmonary cryptococcal infections and to analyse the relevant factors. The chisquared test was used to test for differences in categorical variables, and the independent samples t test was used to compare continuous variables. Multivariable analyses using the Cox proportional hazards model were used to estimate the effect of prognostic factors on treatment time and improvement time. A total of 243 patients were included in the analysis. Immunocompetent patients with diffuse imaging infiltrates had an extension of the treatment course within six months (P = 0.048) and an extension of the improvement days within four weeks (P = 0.008). In immunocompromised patients, an antigen assay ≥ 40 (P = 0.013) is an unfavourable factor leading to an extension of treatment by nine months. The serum antigen assay in 26/98 (26.53%) immunocompetent patients who did not turn negative when the treatment had finished was significantly lower than that in 14/29 (48.28%) immunocompromised patients (P = 0.027). All patients who underwent surgical resection had a good prognosis. Diffuse imaging infiltrates suggest longer treatment days and a longer improvement time in immunocompetent patients. Higher serum antigen levels in immunocompromised patients indicate longer treatment. Serum antigen assays in immunocompromised patients are difficult to negative.

Numerous medications have been associated with an increased risk of vaginal hemorrhage in women. In this study, we analyzed data from the FDA Adverse Event Reporting System (FAERS), focusing on reports of drug-induced vaginal bleeding in women. Risk signals were assessed using disproportionality analyses, specifically the reporting odds ratio (ROR) and the proportional reporting ratio (PRR), to identify significant associations between drugs and adverse events. We found that anticoagulants, hormonal drugs, psychotropic drugs, hypoglycemic agents, antineoplastic agents, anti-inflammatory drugs, immunological agents, and some drugs for osteoporosis were significantly associated with the risk of vaginal hemorrhage. Hormonal drugs, anticoagulants, and particularly antifungal agents were attributed to a notably high proportion of vaginal hemorrhage cases, necessitating further investigation into the underlying mechanisms. Therefore, precise clinical management of medications and optimization of treatment regimens are necessary to reduce the risk of vaginal hemorrhage and improve safety.

Fosfomycin, with its unique mechanism of action, has emerged as a promising option for clinicians to combat antimicrobial resistance and the limited availability of effective drugs, which has led to an increase in associated adverse events (AEs). This study aims to explore the AEs caused by fosfomycin through data mining of the US FDA Adverse Event Reporting System (FAERS) to inform clinical safety. As revealed by FAERS, the 796 fosfomycin-associated AEs occurred more commonly in females (61.90%), with Italy reporting the highest incidence (32.40%), and have a significant rise with peak years in 2018 and 2019. The analysis revealed that gastrointestinal disorders, injury, poisoning and procedural complications, and skin and subcutaneous tissue disorders were among the most commonly reported system organ classes (SOCs), accounting for 16.29%, 13.50%, and 11.26% of cases, respectively. The median time to onset (TTO) for fosfomycin associated AEs was 2 days, indicating an early failure type distribution. Off-label use, diarrhoea, and nausea were among the top 50 most frequent AEs, with reporting odds ratios (RORs) of 3.39, 3.87, and 1.79, respectively. These findings emphasize the need for careful monitoring of fosfomycin use, particularly among female patients and in high-reporting regions. The unique profile of fosfomycin associated AEs identified in this analysis calls for a reevaluation of existing safety profiles, as it may differ from previous studies and product labeling. Our findings offer important insights for medical and public health fields, and are essential for enhancing pharmacovigilance and refining clinical management.