Mutation of OsCDS5 confers broad-spectrum disease resistance in rice

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES Molecular plant pathology Pub Date : 2024-02-07 DOI:10.1111/mpp.13430
Qiping Sun, Yongxin Xiao, Le Song, Lei Yang, Yin Wang, Wei Yang, Qun Yang, Kabin Xie, Meng Yuan, Guotian Li
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Abstract

Phospholipids are important components of biological membranes, participating in various biological processes, including plant development and responses to biotic and abiotic stresses. A previous study showed that mutation of the rice OsCDS5 (CDP-DAG Synthase) gene alters lipid metabolism, causing enhanced abiotic stress responses, yellowing of leaves at the seedling stage and delayed plant development. Here, we observed that the Oscds5 mutant shows enhanced resistance to rice blast, bacterial blight and bacterial leaf streak. Mutation of OsCDS5 promotes production of reactive oxygen species and increases the expression level of multiple defence-related genes. Transcriptomic analyses indicate that genes involved in responses to stress, biotic/abiotic stimuli and metabolic processes are highly upregulated and enriched in mutant Oscds5. Metabolomic analyses showed that differential metabolites were enriched in the lipid metabolic and tryptophan metabolic pathways. The decreased level of phosphatidylinositol and increased level of serotonin probably contribute to enhanced disease resistance of the Oscds5 mutant. Taken together, mutation of OsCDS5 enhances abiotic and biotic stress responses, and OsCDS5 may be a promising target for genetic engineering to enhance the resilience of rice to abiotic and biotic stresses simultaneously.
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突变 OsCDS5 赋予水稻广谱抗病性
磷脂是生物膜的重要组成部分,参与各种生物过程,包括植物的生长发育以及对生物和非生物胁迫的反应。之前的一项研究表明,水稻 OsCDS5(CDP-DAG 合成酶)基因突变会改变脂质代谢,导致非生物胁迫响应增强、苗期叶片变黄和植株发育延迟。在这里,我们观察到 Oscds5 突变体对稻瘟病、细菌性枯萎病和细菌性叶斑病的抗性增强。OsCDS5 的突变促进了活性氧的产生,并提高了多个防御相关基因的表达水平。转录组分析表明,在突变体 Oscds5 中,涉及对胁迫、生物/非生物刺激和代谢过程反应的基因被高度上调和富集。代谢组分析表明,在脂质代谢和色氨酸代谢途径中富集了不同的代谢物。磷脂酰肌醇水平的降低和血清素水平的升高可能有助于增强 Oscds5 突变体的抗病性。综上所述,OscCDS5突变增强了非生物和生物胁迫响应,OscCDS5可能是同时增强水稻对非生物和生物胁迫的抗逆性的一个有希望的基因工程靶标。
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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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