Effects of a novel bioprocess for the cultivation Synechococcus nidulans on Mars on its biochemical composition: focus on the lipidome

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioprocess Engineering Pub Date : 2024-02-12 DOI:10.1007/s12257-024-00019-3
Mattia Casula, Giacomo Fais, Agnieszka Sidorowicz, Pierluigi Caboni, Giacomo Cao, Alessandro Concas
{"title":"Effects of a novel bioprocess for the cultivation Synechococcus nidulans on Mars on its biochemical composition: focus on the lipidome","authors":"Mattia Casula, Giacomo Fais, Agnieszka Sidorowicz, Pierluigi Caboni, Giacomo Cao, Alessandro Concas","doi":"10.1007/s12257-024-00019-3","DOIUrl":null,"url":null,"abstract":"<p>In the present work, the possibility to grow the strain <i>Synechococcus nidulans</i> CCALA 188 on Mars using a medium mimicking a one obtainable using in situ available resources, i.e. the so-called Martian medium, under an atmosphere obtainable by pressurization of Mars CO<sub>2</sub>, is investigated. The goal is to obtain a biomass with high-value products to sustain a crewed mission to Mars. The results show that the replacement of 40% vol of Z-medium with the same volume of Martian medium does not affect the cultivation and leads to a slight improvement of biomass productivity. Under an atmosphere consisting of pure CO<sub>2</sub> the growth rate was reduced but the strain managed to adapt by modifying its metabolism. Total proteins and carbohydrates were significantly reduced under Mars-like conditions, while lipids increased when using CO<sub>2</sub>. A balanced diet rich in antioxidants is crucial for the wealth of astronauts, and in our case, radical scavenging capacities range from 15 to 20 mmol<sub>TEAC</sub>/kg were observed. Under CO<sub>2</sub>, a reduction in antioxidant power is observed likely due to a decrease in photosynthetic activity. The lipidome consisted of sulfoquinovosyldiacylglycerol, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylcholine, phosphatidylglycerol, and triacylglycerol. A significant increase in the latter ones was observed under Mars simulated atmosphere.</p>","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology and Bioprocess Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s12257-024-00019-3","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

In the present work, the possibility to grow the strain Synechococcus nidulans CCALA 188 on Mars using a medium mimicking a one obtainable using in situ available resources, i.e. the so-called Martian medium, under an atmosphere obtainable by pressurization of Mars CO2, is investigated. The goal is to obtain a biomass with high-value products to sustain a crewed mission to Mars. The results show that the replacement of 40% vol of Z-medium with the same volume of Martian medium does not affect the cultivation and leads to a slight improvement of biomass productivity. Under an atmosphere consisting of pure CO2 the growth rate was reduced but the strain managed to adapt by modifying its metabolism. Total proteins and carbohydrates were significantly reduced under Mars-like conditions, while lipids increased when using CO2. A balanced diet rich in antioxidants is crucial for the wealth of astronauts, and in our case, radical scavenging capacities range from 15 to 20 mmolTEAC/kg were observed. Under CO2, a reduction in antioxidant power is observed likely due to a decrease in photosynthetic activity. The lipidome consisted of sulfoquinovosyldiacylglycerol, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylcholine, phosphatidylglycerol, and triacylglycerol. A significant increase in the latter ones was observed under Mars simulated atmosphere.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
用于在火星上培养 Synechococcus nidulans 的新型生物工艺对其生化组成的影响:重点关注脂质组
在本研究工作中,研究了在火星二氧化碳加压产生的大气环境下,使用模拟可利用现场可用资源获得的培养基(即所谓的火星培养基)在火星上培养 Synechococcus nidulans CCALA 188 菌株的可能性。目标是获得一种具有高价值产品的生物质,以维持火星乘员任务。结果表明,用相同体积的火星培养基替代 40% 的 Z 培养基不会影响培养效果,反而会略微提高生物质的生产率。在纯二氧化碳环境下,生长速度降低,但菌株通过改变新陈代谢得以适应。在类似火星的条件下,总蛋白质和碳水化合物明显减少,而在使用二氧化碳时,脂质则有所增加。富含抗氧化剂的均衡饮食对宇航员的财富至关重要,在我们的实验中,观察到自由基清除能力为 15 至 20 毫摩尔TEAC/千克。在二氧化碳环境下,可能由于光合作用减弱,抗氧化能力下降。脂质体包括磺基喹诺酮酰基二乙酰甘油、单半乳糖酰基二乙酰甘油、二半乳糖酰基二乙酰甘油、磷脂酰胆碱、磷脂酰甘油和三酰甘油。在火星模拟大气中观察到后一种物质明显增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
期刊最新文献
Assessing the applicability of tunicate skin-extracted cellulose as a base material for ultrasound gel Fabrication of protein–inorganic biohybrid as an imageable drug delivery system comprising transferrin, green fluorescent protein, and copper phosphate Continuous cell recycling in methylotrophic yeast Pichia pastoris to enhance product yields: a case study with Yarrowia lipolytica lipase Lip2 Sensitive detection of SARS-CoV2 spike antibodies by a paper-based polypyrrole/reduced graphene oxide sensor A neural ordinary differential equation model for predicting the growth of Chinese Hamster Ovary cell in a bioreactor system
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1