Xylanase enzyme from novel strain and its immobilization onto metal organic framework MOF for fruit juice clarification

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioprocess Engineering Pub Date : 2024-02-12 DOI:10.1007/s12257-024-00007-7
Jyoti Kaushal, Madhu Khatri, Gursharan Singh, Shailendra Kumar Arya
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Abstract

Bacillus pumilus, a bacterial strain was isolated from agricultural soil and used for xylanase enzyme (Xy) production under the submerged fermentation technique. The (Xy) enzyme had an optimum temperature at 50℃ (maximum activity from 45–60 °C) and was active at broad pH range (5.0–8.0) with an optimum pH at around 6.3 as evaluated from response surface methodology studies. This enzyme after purification (purification; 2.87 folds, specific activity; 64.3 U/mg) was immobilized onto MOFCu-BTC (a copper ion-based metal organic framework) and was used for clarification of freshly squeezed fruit juice (pineapple and pomegranate). The study revealed an improved catalytic efficiency (Vmax from 1.252.5 to 1.361 U/mL/mg of support) and greater half-life of the immobilized system (77–99 min). The activation energy decreased from that required for the free system (37.59–25.63 kJ/mol). The reusability of the enzyme improved after immobilization where 61% of the enzyme’s activity was retained after 21 cycles of usage. The MOFXy-Cu-BTC system showed improved clarification (47.58–57.97% for pineapple, and 15.34–18.3 for pomegranate) thereby showing its effectiveness in commercial juice clarification process.

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来自新型菌株的木聚糖酶及其在金属有机框架 MOF 上的固定化用于果汁澄清
从农业土壤中分离出一种细菌菌株--枯草芽孢杆菌(Bacillus pumilus),并采用浸没发酵技术生产木聚糖酶(Xy)。根据响应面方法学研究的评估,木聚糖酶(Xy)的最适温度为 50℃(45-60℃时活性最高),在广泛的 pH 值范围(5.0-8.0)内均有活性,最适 pH 值约为 6.3。纯化后的这种酶(纯化倍数为 2.87 倍,比活性为 64.3 U/mg )被固定在 MOFCu-BTC(一种基于铜离子的金属有机框架)上,用于澄清鲜榨果汁(菠萝和石榴)。研究结果表明,固定化系统的催化效率提高了(Vmax 从 1.252.5 U/mg/支持物提高到 1.361 U/mg/支持物),半衰期延长了(77-99 min)。活化能与游离体系相比有所降低(37.59-25.63 kJ/mol)。固定化后,酶的可再利用性得到了改善,在使用 21 个周期后,61% 的酶活性得以保留。MOFXy-Cu-BTC 系统提高了澄清度(菠萝为 47.58%-57.97%,石榴为 15.34%-18.3%),从而显示了其在商业果汁澄清过程中的有效性。
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来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
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