{"title":"Effect of the hps/phi Copy Number on the Growth of Methylococcus capsulatus MIR","authors":"","doi":"10.1134/s0026261723603536","DOIUrl":null,"url":null,"abstract":"<span> <h3> <strong>Abstract</strong>—</h3> <p><em>Methylococcus capsulatus</em> MIR is a biotechnologically promising strain of methanotrophic bacteria for obtaining feed protein and other value-added products from methane. This strain assimilates methane carbon mainly via the ribulose monophosphate pathway, the key enzymes of which are 3-hexulose-6-phosphate synthase and 6-phospho-3-hexulose isomerase. It was shown that the presence of one, two, or three copies of the genes encoding hexulose phosphate synthase/isomerase affected the level of the total gene expression but did not affect the growth characteristics of the strain and did not correlate with the protein content in the cells. The data obtained indicate that fixation of formaldehyde by hexulose phosphate synthase is not the reaction limiting the growth of bacteria of the genus <em>Methylococcus</em>.</p> </span>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0026261723603536","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract—
Methylococcus capsulatus MIR is a biotechnologically promising strain of methanotrophic bacteria for obtaining feed protein and other value-added products from methane. This strain assimilates methane carbon mainly via the ribulose monophosphate pathway, the key enzymes of which are 3-hexulose-6-phosphate synthase and 6-phospho-3-hexulose isomerase. It was shown that the presence of one, two, or three copies of the genes encoding hexulose phosphate synthase/isomerase affected the level of the total gene expression but did not affect the growth characteristics of the strain and did not correlate with the protein content in the cells. The data obtained indicate that fixation of formaldehyde by hexulose phosphate synthase is not the reaction limiting the growth of bacteria of the genus Methylococcus.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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